Literature DB >> 26096505

Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads.

Erica Gerace1, Danesh Moazed2.   

Abstract

FLAG is an affinity tag widely used for rapid and highly specific one-step protein purification. Native elution of protein from anti-FLAG antibody resins allows the identification of protein and nucleic acid binding partners and functional analysis using biochemical activity assays.
© 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Anti-FLAG M2 agarose beads; Anti-FLAG tag antibody; Cell lysates; FLAG; Immunoprecipitation; Mock-Elution; Peptide elution

Mesh：

Substances：

Year:  2015        PMID： 26096505      PMCID： PMC4794115          DOI： 10.1016/bs.mie.2014.11.010

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  5 in total

Review 1.  The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins.

Authors:  A Einhauer; A Jungbauer
Journal:  J Biochem Biophys Methods       Date:  2001-10-30

2.  Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast.

Authors:  Shane M Buker; Tetsushi Iida; Marc Bühler; Judit Villén; Steven P Gygi; Jun-Ichi Nakayama; Danesh Moazed
Journal:  Nat Struct Mol Biol       Date:  2007-02-25       Impact factor: 15.369

3.  Monoclonal anti-FLAG antibodies react with a new isoform of rat Mg2+ dependent protein phosphatase beta.

Authors:  K Schäfer; T Braun
Journal:  Biochem Biophys Res Commun       Date:  1995-02-15       Impact factor: 3.575

4.  The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.

Authors:  Erica L Gerace; Mario Halic; Danesh Moazed
Journal:  Mol Cell       Date:  2010-08-13       Impact factor: 17.970

5.  Dicer-independent primal RNAs trigger RNAi and heterochromatin formation.

Authors:  Mario Halic; Danesh Moazed
Journal:  Cell       Date:  2010-02-19       Impact factor: 41.582
  5 in total
  8 in total

1.  The RNA degradosome promotes tRNA quality control through clearance of hypomodified tRNA.

Authors:  Satoshi Kimura; Matthew K Waldor
Journal:  Proc Natl Acad Sci U S A       Date:  2019-01-08       Impact factor: 11.205

2.  Lung cancer: progression of heat shock protein 70 in association with flap endonuclease 1 protein.

Authors:  Chandra Sekhar Kathera; Jiang Longwei; Avilala Janardhan; Lihong Qin; Qi Zhang; Wu Lan; Jia Shaochang; Zhigang Guo
Journal:  3 Biotech       Date:  2021-02-25       Impact factor: 2.406

3.  PG1659 functions as anti-sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83.

Authors:  Yuetan Dou; Hiel Rutanhira; Norbert Schormann; Champion Deivanayagam; Hansel M Fletcher
Journal:  Mol Oral Microbiol       Date:  2021-01-13       Impact factor: 4.107

Review 4.  An Overview of Current Methods to Confirm Protein-Protein Interactions.

Authors:  Kenji Miura
Journal:  Protein Pept Lett       Date:  2018       Impact factor: 1.890

5.  Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain-specific cargo.

Authors:  David Rufino-Ramos; Sevda Lule; Shadi Mahjoum; Stefano Ughetto; D Cristopher Bragg; Luís Pereira de Almeida; Xandra O Breakefield; Koen Breyne
Journal:  Biomaterials       Date:  2022-01-08       Impact factor: 12.479

6.  Examining the Protein Interactome and Subcellular Localization of RNase J2 Complexes in Streptococcus mutans.

Authors:  Rong Mu; Pushkar Shinde; Zhengzhong Zou; Jens Kreth; Justin Merritt
Journal:  Front Microbiol       Date:  2019-09-18       Impact factor: 5.640

7.  GLP-1 Notch-LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1.

Authors:  Jian Chen; Ariz Mohammad; Nanette Pazdernik; Huiyan Huang; Beth Bowman; Eric Tycksen; Tim Schedl
Journal:  PLoS Genet       Date:  2020-03-20       Impact factor: 5.917

8.  Activation of mTORC1 by LSECtin in macrophages directs intestinal repair in inflammatory bowel disease.

Authors:  Qian Li; Hanxing Cheng; Yuanping Liu; Xiaowen Wang; Fuchu He; Li Tang
Journal:  Cell Death Dis       Date:  2020-10-26       Impact factor: 8.469
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.