Literature DB >> 7532404

Monoclonal anti-FLAG antibodies react with a new isoform of rat Mg2+ dependent protein phosphatase beta.

K Schäfer1, T Braun.   

Abstract

The FLAG peptide has been widely used as a multi-purpose tag for the identification and detection of recombinant FLAG fusion proteins. The practicability of this approach depends on specific detection of FLAG fusion proteins with no or very little cross-reactivity to cellular proteins. We have isolated a rat cDNA clone coding for a new splicing isoform of Mg2+ dependent protein phosphatase beta (MPP beta) by screening a rat brain expression library with monoclonal antibody Anti-FLAG M2. MPP beta reacts strongly both as a MPP beta-beta-galactosidase- and as a glutathione S-transferase fusion protein with anti-FLAG M2 antibodies. Sequence analysis of MPP beta revealed a sequence motif with five out of eight amino acid residues identical to the FLAG peptide hitherto believed to be mono-specific.

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Year:  1995        PMID： 7532404     DOI： 10.1006/bbrc.1995.1245

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  The polarity protein VANG-1 antagonizes Wnt signaling by facilitating Frizzled endocytosis.

Authors:  Chun-Wei He; Chien-Po Liao; Chung-Kuan Chen; Jérôme Teulière; Chun-Hao Chen; Chun-Liang Pan
Journal:  Development       Date:  2018-12-17       Impact factor: 6.868

2.  Involvement of a Golgi-resident GPI-anchored protein in maintenance of the Golgi structure.

Authors:  Xueyi Li; Dora Kaloyanova; Martin van Eijk; Ruud Eerland; Gisou van der Goot; Viola Oorschot; Judith Klumperman; Friedrich Lottspeich; Vytaute Starkuviene; Felix T Wieland; J Bernd Helms
Journal:  Mol Biol Cell       Date:  2007-01-24       Impact factor: 4.138

3.  Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5.

Authors:  J W Slootstra; D Kuperus; A Plückthun; R H Meloen
Journal:  Mol Divers       Date:  1997       Impact factor: 2.943

4.  Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads.

Authors:  Erica Gerace; Danesh Moazed
Journal:  Methods Enzymol       Date:  2015-05-16       Impact factor: 1.600

5.  Immunohistochemical detection of FLAG-tagged endogenous proteins in knock-in mice.

Authors:  Ronald E Ferrando; Kim Newton; Felix Chu; Joshua D Webster; Dorothy M French
Journal:  J Histochem Cytochem       Date:  2015-01-09       Impact factor: 2.479

6.  G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity.

Authors:  Kasumi Tatsumi; Gyosuke Sakashita; Yuko Nariai; Kosuke Okazaki; Hiroaki Kato; Eiji Obayashi; Hisashi Yoshida; Kanako Sugiyama; Sam-Yong Park; Joji Sekine; Takeshi Urano
Journal:  Sci Rep       Date:  2017-03-07       Impact factor: 4.379

Review 7.  Knock-In Mouse Models to Investigate the Functions of Opioid Receptors in vivo.

Authors:  Jade Degrandmaison; Samuel Rochon-Haché; Jean-Luc Parent; Louis Gendron
Journal:  Front Cell Neurosci       Date:  2022-01-31       Impact factor: 5.505
  7 in total

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