OBJECTIVE: to investigate the associations of oral microbiota, leucocytes count, neutrophil count, platelet counts and hemoglobin level, and the severity of oral mucositis in pediatric patients with acute lymphoblastic leukemia (ALL) receiving chemotherapy. MATERIALS AND METHODS: 71 prospective patients were included. Analyses of oral microbiota and blood sample were conducted on days 14 (D14) and 56 (D56) of the Brazilian GBTLI-99 treatment protocol. Herpes simplex virus (HSV) identification was performed by PCR followed by DNA sequencing analysis. Bacteria and fungi identification was obtained by standard microbiological culture tests. RESULTS: 103 episodes of mucositis occurred, being 65 at D14 and 38 at D56. Most cases positive for herpes viral DNA sequences were identified as HSV-1. At D14, we found a significant association between the severity of mucositis and presence of HSV-1 (p = 0.0347), Candida spp. (p = 0.0078), and low platelet count (p = 0.0064). At D56, we found a significant association between the severity of mucositis and the presence of HSV-1 (p = 0.0317), previous HSV-1 presence on D14 (p < 0.0001) and neutrophil count (p = 0.0211). CLINICAL RELEVANCE: the identification of risk factors for mucositis in children and adolescents may contribute to the development of new strategies for prevention and/or treatment, reducing the complications associated with this condition. CONCLUSIONS: the presence of HSV, platelet count, and Candida spp. presence at D14 of ALL induction treatment is associated with increased severity of mucositis in children and adolescents. At D56 of ALL treatment, mucositis severity was associated with neutrophil count, HSV presence, and previous presence of HSV (at D14).
OBJECTIVE: to investigate the associations of oral microbiota, leucocytes count, neutrophil count, platelet counts and hemoglobin level, and the severity of oral mucositis in pediatric patients with acute lymphoblastic leukemia (ALL) receiving chemotherapy. MATERIALS AND METHODS: 71 prospective patients were included. Analyses of oral microbiota and blood sample were conducted on days 14 (D14) and 56 (D56) of the Brazilian GBTLI-99 treatment protocol. Herpes simplex virus (HSV) identification was performed by PCR followed by DNA sequencing analysis. Bacteria and fungi identification was obtained by standard microbiological culture tests. RESULTS: 103 episodes of mucositis occurred, being 65 at D14 and 38 at D56. Most cases positive for herpes viral DNA sequences were identified as HSV-1. At D14, we found a significant association between the severity of mucositis and presence of HSV-1 (p = 0.0347), Candida spp. (p = 0.0078), and low platelet count (p = 0.0064). At D56, we found a significant association between the severity of mucositis and the presence of HSV-1 (p = 0.0317), previous HSV-1 presence on D14 (p < 0.0001) and neutrophil count (p = 0.0211). CLINICAL RELEVANCE: the identification of risk factors for mucositis in children and adolescents may contribute to the development of new strategies for prevention and/or treatment, reducing the complications associated with this condition. CONCLUSIONS: the presence of HSV, platelet count, and Candida spp. presence at D14 of ALL induction treatment is associated with increased severity of mucositis in children and adolescents. At D56 of ALL treatment, mucositis severity was associated with neutrophil count, HSV presence, and previous presence of HSV (at D14).
Authors: Camila Weissheimer; Marina Curra; Lauro J Gregianin; Liane E Daudt; Vivian P Wagner; Marco Antonio T Martins; Manoela D Martins Journal: Lasers Med Sci Date: 2017-08-24 Impact factor: 3.161
Authors: Rafaela Maia Almendra Mattos; Regina Maria Holanda de Mendonça; Simone Dos Santos Aguiar Journal: Support Care Cancer Date: 2019-05-23 Impact factor: 3.603
Authors: Marina Curra; Amanda F Gabriel; Maria Beatriz C Ferreira; Marco Antonio T Martins; André T Brunetto; Lauro J Gregianin; Manoela Domingues Martins Journal: Support Care Cancer Date: 2021-04-12 Impact factor: 3.603
Authors: Camila Stofella Sodré; Paulo Matheus Guerra Rodrigues; Mayra Stambovsky Vieira; Alexandre Marques Paes da Silva; Lucio Souza Gonçalves; Marcia Gonçalves Ribeiro; Dennis de Carvalho Ferreira Journal: J Oral Microbiol Date: 2020-08-17 Impact factor: 5.474
Authors: Sali Al-Ansari; Judith A E M Zecha; Andrei Barasch; Jan de Lange; Fred R Rozema; Judith E Raber-Durlacher Journal: Curr Oral Health Rep Date: 2015-10-19