Literature DB >> 26060080

Coupling Binding to Catalysis: Using Yeast Cell Surface Display to Select Enzymatic Activities.

Keya Zhang1, Karan Bhuripanyo, Yiyang Wang, Jun Yin.   

Abstract

We find yeast cell surface display can be used to engineer enzymes by selecting the enzyme library for high affinity binding to reaction intermediates. Here we cover key steps of enzyme engineering on the yeast cell surface including library design, construction, and selection based on magnetic and fluorescence-activated cell sorting.

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Year:  2015        PMID： 26060080      PMCID： PMC4648535          DOI： 10.1007/978-1-4939-2748-7_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Engineering antibody affinity by yeast surface display.

Authors:  David W Colby; Brenda A Kellogg; Christilyn P Graff; Yik A Yeung; Jeffrey S Swers; K Dane Wittrup
Journal:  Methods Enzymol       Date:  2004       Impact factor: 1.600

Review 2.  Lessons from natural molecules.

Authors:  Jon Clardy; Christopher Walsh
Journal:  Nature       Date:  2004-12-16       Impact factor: 49.962

3.  Rationally designed nucleoside antibiotics that inhibit siderophore biosynthesis of Mycobacterium tuberculosis.

Authors:  Ravindranadh V Somu; Helena Boshoff; Chunhua Qiao; Eric M Bennett; Clifton E Barry; Courtney C Aldrich
Journal:  J Med Chem       Date:  2006-01-12       Impact factor: 7.446

4.  Isolating and engineering human antibodies using yeast surface display.

Authors:  Ginger Chao; Wai L Lau; Benjamin J Hackel; Stephen L Sazinsky; Shaun M Lippow; K Dane Wittrup
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

5.  Gene splicing by overlap extension.

Authors:  R M Horton; S N Ho; J K Pullen; H D Hunt; Z Cai; L R Pease
Journal:  Methods Enzymol       Date:  1993       Impact factor: 1.600

6.  Yeast surface display for directed evolution of protein expression, affinity, and stability.

Authors:  E T Boder; K D Wittrup
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

7.  Crystal structure of DhbE, an archetype for aryl acid activating domains of modular nonribosomal peptide synthetases.

Authors:  Jurgen J May; Nadine Kessler; Mohamed A Marahiel; Milton T Stubbs
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-09       Impact factor: 11.205

8.  Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display.

Authors:  Dasa Lipovsek; Eugene Antipov; Kathryn A Armstrong; Mark J Olsen; Alexander M Klibanov; Bruce Tidor; K Dane Wittrup
Journal:  Chem Biol       Date:  2007-10

Review 9.  Harnessing the biosynthetic code: combinations, permutations, and mutations.

Authors:  D E Cane; C T Walsh; C Khosla
Journal:  Science       Date:  1998-10-02       Impact factor: 47.728

Review 10.  Yeast surface display for protein engineering and characterization.

Authors:  S Annie Gai; K Dane Wittrup
Journal:  Curr Opin Struct Biol       Date:  2007-09-17       Impact factor: 6.809
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