BACKGROUND: Sample stability is a crucial aspect for the quality of results of a haematology laboratory. This study was conducted to investigate the reliability of haematological testing using Sysmex XN in samples stored for up to 24 h at different temperatures. MATERIALS AND METHODS: Haematological tests were performed on whole blood samples collected from 16 ostensibly healthy outpatients immediately after collection and 3 h, 6 h or 24 h afterwards, with triple aliquots kept at room temperature, 4 °C or 37 °C. RESULTS: No meaningful bias was observed after 3 h under different storage conditions, except for red blood cell distribution width (RDW) and platelet count (impedance technique, PLT-I) at 37 °C. After 6 h, meaningful bias was observed for mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) at room temperature, red blood cell (RBC) count, mean corpuscular haemoglobin concentration (MCHC), MCH, MCV and PLT-I at 4 °C, and RBC, RDW, MCHC, MCH and PLT-I at 37 °C. After 24 h, a meaningful bias was observed for MCHC, MCV, platelet count (fluorescent technique, PLT-F) and mean platelet volume (MPV) at room temperature, MCHC, MCV, PLT-I and MPV at 4 °C, and all parameters except RBC count and MPV at 37 °C. DISCUSSION: Great caution should be observed when analysing results of haematological tests conducted more than 3 h after sample collection.
BACKGROUND: Sample stability is a crucial aspect for the quality of results of a haematology laboratory. This study was conducted to investigate the reliability of haematological testing using Sysmex XN in samples stored for up to 24 h at different temperatures. MATERIALS AND METHODS: Haematological tests were performed on whole blood samples collected from 16 ostensibly healthy outpatients immediately after collection and 3 h, 6 h or 24 h afterwards, with triple aliquots kept at room temperature, 4 °C or 37 °C. RESULTS: No meaningful bias was observed after 3 h under different storage conditions, except for red blood cell distribution width (RDW) and platelet count (impedance technique, PLT-I) at 37 °C. After 6 h, meaningful bias was observed for mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) at room temperature, red blood cell (RBC) count, mean corpuscular haemoglobin concentration (MCHC), MCH, MCV and PLT-I at 4 °C, and RBC, RDW, MCHC, MCH and PLT-I at 37 °C. After 24 h, a meaningful bias was observed for MCHC, MCV, platelet count (fluorescent technique, PLT-F) and mean platelet volume (MPV) at room temperature, MCHC, MCV, PLT-I and MPV at 4 °C, and all parameters except RBC count and MPV at 37 °C. DISCUSSION: Great caution should be observed when analysing results of haematological tests conducted more than 3 h after sample collection.
Authors: C Ricós; V Alvarez; F Cava; J V García-Lario; A Hernández; C V Jiménez; J Minchinela; C Perich; M Simón Journal: Scand J Clin Lab Invest Date: 1999-11 Impact factor: 1.713
Authors: Giuseppe Lippi; Giuseppe Banfi; Mauro Buttarello; Ferruccio Ceriotti; Massimo Daves; Alberto Dolci; Marco Caputo; Davide Giavarina; Martina Montagnana; Valentino Miconi; Bruno Milanesi; Andrea Mosca; Margherita Morandini; Gian Luca Salvagno Journal: Clin Chem Lab Med Date: 2007 Impact factor: 3.694
Authors: Gabriel Lima-Oliveira; Gian Cesare Guidi; Gian Luca Salvagno; Elisa Danese; Martina Montagnana; Giuseppe Lippi Journal: Rev Bras Hematol Hemoter Date: 2017-02-22