Literature DB >> 2604702

A kinetic investigation of the acyl-CoA oxidase reaction with the use of a novel spectrophotometric assay. Inhibition by acetyl-CoA, CoA and FMN.

R Hovik1, H Osmundsen.   

Abstract

A direct-reading spectrophotometric assay for acyl-CoA oxidase activity is described. The assay is based on the strong absorption at 300 nm of deca-2-trans,4-cis-dienoyl-CoA, the product of oxidation of dec-4-cis-enoyl-CoA. By use of this assay, acetyl-CoA, CoA and FMN were found to be inhibitors of acyl-CoA oxidase, but with distinctly different kinetic characteristics.

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Year:  1989        PMID： 2604702      PMCID： PMC1133424          DOI： 10.1042/bj2630297

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  Peroxisomal oxidation of long chain fatty acids.

Authors:  H Osmundsen; C E Neat; K R Norum
Journal:  FEBS Lett       Date:  1979-03-15       Impact factor: 4.124

2.  Chain length specificities of peroxisomal and mitochondrial beta-oxidation in rat liver.

Authors:  D J Hryb; J F Hogg
Journal:  Biochem Biophys Res Commun       Date:  1979-04-27       Impact factor: 3.575

3.  Beta oxidation in glyoxysomes from castor bean endosperm.

Authors:  T G Cooper; H Beevers
Journal:  J Biol Chem       Date:  1969-07-10       Impact factor: 5.157

4.  Detection of peroxisomal fatty acyl-coenzyme A oxidase activity.

Authors:  N C Inestrosa; M Bronfman; F Leighton
Journal:  Biochem J       Date:  1979-09-15       Impact factor: 3.857

5.  Purification of the peroxisomal fatty acyl-CoA oxidase from rat liver.

Authors:  N C Inestrosa; M Bronfman; F Leighton
Journal:  Biochem Biophys Res Commun       Date:  1980-07-16       Impact factor: 3.575

6.  A convenient computer program for fitting enzymatic rate laws to steady-state data.

Authors:  M C Kohn; L E Menten; D Garfinkel
Journal:  Comput Biomed Res       Date:  1979-10

7.  Purification and properties of acyl-CoA oxidase from rat liver.

Authors:  T Osumi; T Hashimoto; N Ui
Journal:  J Biochem       Date:  1980-06       Impact factor: 3.387

8.  A luminometric assay for peroxisomal beta-oxidation. Effects of fasting and streptozotocin-diabetes on peroxisomal beta-oxidation.

Authors:  H Osmundsen; B Brodal; R Hovik
Journal:  Biochem J       Date:  1989-05-15       Impact factor: 3.857

9.  A fatty acyl-CoA oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drug.

Authors:  P B Lazarow; C De Duve
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

10.  Effects of high-fat diets on hepatic fatty acid oxidation in the rat. Isolation of rat liver peroxisomes by vertical-rotor centrifugation by using a self-generated, iso-osmotic, Percoll gradient.

Authors:  C E Neat; M S Thomassen; H Osmundsen
Journal:  Biochem J       Date:  1981-04-15       Impact factor: 3.857
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  5 in total

1.  Effects of thia-substituted fatty acids on mitochondrial and peroxisomal beta-oxidation. Studies in vivo and in vitro.

Authors:  R Hovik; H Osmundsen; R Berge; A Aarsland; S Bergseth; J Bremer
Journal:  Biochem J       Date:  1990-08-15       Impact factor: 3.857

2.  Factors which affect the activity of purified rat liver acyl-CoA oxidase.

Authors:  R Hovik; H Osmundsen
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

3.  Products and intermediates of the beta-oxidation of [U-14C]hexadecanedionoyl-mono-CoA by rat liver peroxisomes and mitochondria.

Authors:  M Pourfarzam; K Bartlett
Journal:  Biochem J       Date:  1991-01-01       Impact factor: 3.857

4.  Estimation of peroxisomal beta-oxidation in rat heart by a direct assay of acyl-CoA oxidase.

Authors:  C Chu; L F Mao; H Schulz
Journal:  Biochem J       Date:  1994-08-15       Impact factor: 3.857

5.  The membrane of peroxisomes in Saccharomyces cerevisiae is impermeable to NAD(H) and acetyl-CoA under in vivo conditions.

Authors:  C W van Roermund; Y Elgersma; N Singh; R J Wanders; H F Tabak
Journal:  EMBO J       Date:  1995-07-17       Impact factor: 11.598
  5 in total

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