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Literature DB >> 26011555

Quantitative tracking of protein trafficking to the nucleus using cytosolic protein delivery by nanoparticle-stabilized nanocapsules.

Moumita Ray¹, Rui Tang¹, Ziwen Jiang¹, Vincent M Rotello¹.

Abstract

We describe a method for quantitative monitoring of subcellular protein trafficking using nanoparticle-stabilized nanocapsules for protein delivery. This method provides rapid delivery of the protein into the cytosol, eliminating complications from protein homeostasis processes found with cellularly expressed proteins. After delivery, nuclear protein trafficking was followed by real time microscopic imaging. Quantitative analyses of the accumulation percentage and the import dynamics of the nuclear protein trafficking, demonstrate the utility of this method for studying intracellular trafficking systems.

Entities: CellLine Chemical Disease Gene Species

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Year: 2015 PMID： 26011555 PMCID： PMC4743495 DOI： 10.1021/acs.bioconjchem.5b00141

Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 4.774

18 in total

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Authors: Mien-Chie Hung; Wolfgang Link
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2. Immunolabeling artifacts and the need for live-cell imaging.

Authors: Ulrike Schnell; Freark Dijk; Klaas A Sjollema; Ben N G Giepmans
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3. Direct cytosolic delivery of siRNA using nanoparticle-stabilized nanocapsules.

Authors: Ying Jiang; Rui Tang; Bradley Duncan; Ziwen Jiang; Bo Yan; Rubul Mout; Vincent M Rotello
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4. Identification of the human c-myc protein nuclear translocation signal.

Authors: C V Dang; W M Lee
Journal: Mol Cell Biol Date: 1988-10 Impact factor: 4.272

5. Kinetic properties of nuclear transport conferred by the retinoblastoma (Rb) NLS.

Authors: Wei Hu; Bruce E Kemp; David A Jans
Journal: J Cell Biochem Date: 2005-07-01 Impact factor: 4.429

6. Tus, an E. coli protein, contains mammalian nuclear targeting and exporting signals.

Authors: Stanislaw J Kaczmarczyk; Kalavathy Sitaraman; Thomas Hill; James L Hartley; Deb K Chatterjee
Journal: PLoS One Date: 2010-01-26 Impact factor: 3.240

7. Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. Implications for immunofluorescence microscopy.

Authors: M A Melan; G Sluder
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8. A code for RanGDP binding in ankyrin repeats defines a nuclear import pathway.

Authors: Min Lu; Jaroslav Zak; Shuo Chen; Luis Sanchez-Pulido; David T Severson; Jane Endicott; Chris P Ponting; Christopher J Schofield; Xin Lu
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9. COG-2, a sox domain protein necessary for establishing a functional vulval-uterine connection in Caenorhabditis elegans.

Authors: W Hanna-Rose; M Han
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10. Protein delivery into live cells by incubation with an endosomolytic agent.

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33 in total

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2. General Strategy for Direct Cytosolic Protein Delivery via Protein-Nanoparticle Co-engineering.

Authors: Rubul Mout; Moumita Ray; Tristan Tay; Kanae Sasaki; Gulen Yesilbag Tonga; Vincent M Rotello
Journal: ACS Nano Date: 2017-06-15 Impact factor: 15.881

3. Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse.

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Journal: Proc Natl Acad Sci U S A Date: 2020-12-14 Impact factor: 11.205

Quantitative tracking of protein trafficking to the nucleus using cytosolic protein delivery by nanoparticle-stabilized nanocapsules.

Review 1. Protein localization in disease and therapy.

2. Immunolabeling artifacts and the need for live-cell imaging.

3. Direct cytosolic delivery of siRNA using nanoparticle-stabilized nanocapsules.

4. Identification of the human c-myc protein nuclear translocation signal.

5. Kinetic properties of nuclear transport conferred by the retinoblastoma (Rb) NLS.

6. Tus, an E. coli protein, contains mammalian nuclear targeting and exporting signals.

7. Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. Implications for immunofluorescence microscopy.

8. A code for RanGDP binding in ankyrin repeats defines a nuclear import pathway.

9. COG-2, a sox domain protein necessary for establishing a functional vulval-uterine connection in Caenorhabditis elegans.

10. Protein delivery into live cells by incubation with an endosomolytic agent.

1. Templated Self-Assembly of a Covalent Polymer Network for Intracellular Protein Delivery and Traceless Release.

2. General Strategy for Direct Cytosolic Protein Delivery via Protein-Nanoparticle Co-engineering.

3. Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse.

Review 4. Intracellular delivery of proteins by nanocarriers.

5. Intracellular Delivery of Nanobodies for Imaging of Target Proteins in Live Cells.

6. Cytosolic delivery of large proteins using nanoparticle-stabilized nanocapsules.

7. Evolution and Clinical Translation of Drug Delivery Nanomaterials.

8. CRISPRed Macrophages for Cell-Based Cancer Immunotherapy.

9. Active Targeting of the Nucleus Using Nonpeptidic Boronate Tags.

Review 10. Protein delivery into cells using inorganic nanoparticle-protein supramolecular assemblies.