| Literature DB >> 25998508 |
Yuping Li1,2, Xiwei Wu3, Hanlin Gao1, Jennifer M Jin1, Arthur X Li4, Young S Kim1, Sumanta K Pal5, Rebecca A Nelson4, Clayton M Lau6, Chao Guo1, Bing Mu3, Jinhui Wang3, Frances Wang1, Jessica Wang1, Yuanyin Zhao2, Wengang Chen1, John J Rossi3, Lawrence M Weiss1, Huiqing Wu1.
Abstract
Piwi-interacting RNAs (piRNAs) are a distinct group of small noncoding RNAs (sncRNAs) that silence transposable genetic elements to protect genome integrity. Because of their limited expression in gonads and sequence diversity, piRNAs remain the most mysterious class of small RNAs. Studies have shown piRNAs are present in somatic cells and dysregulated in gastric, breast and liver cancers. By deep sequencing 24 frozen benign kidney and clear cell renal cell carcinoma (ccRCC) specimens and using the publically available piRNA database, we found 26,991 piRNAs present in human kidney tissue. Among 920 piRNAs that had at least two copies in one specimen, 19 were differentially expressed in benign kidney and ccRCC tissues, and 46 were associated with metastasis. Among the metastasis-related piRNAs, we found three piRNAs (piR-32051, piR-39894 and piR-43607) to be derived from the same piRNA cluster at chromosome 17. We confirmed the three selected piRNAs not to be miRNAs or miRNA-like sncRNAs. We further validated the aberrant expression of the three piRNAs in a 68-case formalin-fixed and paraffin-embedded (FFPE) ccRCC tissue cohort and showed the up-regulation of the three piRNAs to be highly associated with ccRCC metastasis, late clinical stage and poor cancer-specific survival.Entities:
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Year: 2015 PMID： 25998508 PMCID： PMC4534471 DOI： 10.2119/molmed.2014.00203
Source DB: PubMed Journal: Mol Med ISSN： 1076-1551 Impact factor: 6.354