| Literature DB >> 25988566 |
René H M Raeven1,2, Larissa van der Maas1, Wichard Tilstra1, Joost P Uittenbogaard1, Tim H E Bindels1, Betsy Kuipers3, Arno van der Ark1, Jeroen L A Pennings4, Elly van Riet1, Wim Jiskoot2, Gideon F A Kersten1,2, Bernard Metz1.
Abstract
The current resurgence of whooping cough is alarming, and improved pertussis vaccines are thought to offer a solution. Outer membrane vesicle vaccines (omvPV) are potential vaccine candidates, but omvPV-induced humoral responses have not yet been characterized in detail. The purpose of this study was to determine the antigen composition of omvPV and to elucidate the immunogenicity of the individual antigens. Quantitative proteome analysis revealed the complex composition of omvPV. The omvPV immunogenicity profile in mice was compared to those of classic whole cell vaccine (wPV), acellular vaccine (aPV), and pertussis infection. Pertussis-specific antibody levels, antibody isotypes, IgG subclasses, and antigen specificity were determined after vaccination or infection by using a combination of multiplex immunoassays, two-dimensional immunoblotting, and mass spectrometry. The vaccines and infection raised strong antibody responses, but large quantitative and qualitative differences were measured. The highest antibody levels were obtained by omvPV. All IgG subclasses (IgG1/IgG2a/IgG2b/IgG3) were elicited by omvPV and in a lower magnitude by wPV, but not by aPV (IgG1) or infection (IgG2a/b). The majority of omvPV-induced antibodies were directed against Vag8, BrkA, and LPS. The broad and balanced humoral response makes omvPV a promising pertussis vaccine candidate.Entities:
Keywords: 2D-electrophoresis; Bordetella pertussis; Western blot; antibody; humoral; outer membrane vesicle; proteomics; subclass; vaccine
Mesh：
Substances：
Year: 2015 PMID： 25988566 DOI： 10.1021/acs.jproteome.5b00258
Source DB: PubMed Journal: J Proteome Res ISSN： 1535-3893 Impact factor: 4.466