| Literature DB >> 28930286 |
R Hm Raeven1,2, J Brummelman3,4, J L A Pennings5, L van der Maas1, K Helm3, W Tilstra1, A van der Ark1, A Sloots1, P van der Ley1, W van Eden4, W Jiskoot2, E van Riet1, C Acm van Els3, G Fa Kersten1,2, W Gh Han3, B Metz1.
Abstract
Mucosal immunity is often required for protection against respiratory pathogens but the underlying cellular and molecular mechanisms of induction remain poorly understood. Here, systems vaccinology was used to identify immune signatures after pulmonary or subcutaneous immunization of mice with pertussis outer membrane vesicles. Pulmonary immunization led to improved protection, exclusively induced mucosal immunoglobulin A (IgA) and T helper type 17 (Th17) responses, and in addition evoked elevated systemic immunoglobulin G (IgG) antibody levels, IgG-producing plasma cells, memory B cells, and Th17 cells. These adaptive responses were preceded by unique local expression of genes of the innate immune response related to Th17 (e.g., Rorc) and IgA responses (e.g., Pigr) in addition to local and systemic secretion of Th1/Th17-promoting cytokines. This comprehensive systems approach identifies the effect of the administration route on the development of mucosal immunity, its importance in protection against Bordetella pertussis, and reveals potential molecular correlates of vaccine immunity to this reemerging pathogen.Entities:
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Year: 2017 PMID： 28930286 DOI： 10.1038/mi.2017.81
Source DB: PubMed Journal: Mucosal Immunol ISSN： 1933-0219 Impact factor: 7.313