Hua Zhao1, Ke Li2, Jia-Yong Tang2, Ji-Chang Zhou3, Kang-Ning Wang2, Xin-Jie Xia4, Xin Gen Lei5. 1. International Center of Future Agriculture for Human Health, Sichuan Agricultural University, Sichuan, China; xl20@cornell.edu zhua666@126.com. 2. International Center of Future Agriculture for Human Health, Sichuan Agricultural University, Sichuan, China; 3. Molecular Biology Laboratory, Shenzhen Center for Chronic Disease Control, Shenzhen, China; 4. International Center of Future Agriculture for Human Health, Sichuan Agricultural University, Sichuan, China; Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China; and. 5. International Center of Future Agriculture for Human Health, Sichuan Agricultural University, Sichuan, China; Department of Animal Science, Cornell University, Ithaca, NY xl20@cornell.edu zhua666@126.com.
Abstract
BACKGROUND: Relations of the 25 mammalian selenoprotein genes with obesity and the associated inflammation remain unclear. OBJECTIVE: This study explored impacts of high-fat diet-induced obesity on inflammation and expressions of selenoprotein and obesity-related genes in 10 tissues of pigs. METHODS: Plasma and 10 tissues were collected from pigs (n = 10) fed a corn-soy-based control diet or that diet containing 3-7% lard from weanling to finishing (180 d). Plasma concentrations (n = 8) of cytokines and thyroid hormones and tissue mRNA abundance (n = 4) of 25 selenoprotein genes and 16 obesity-related genes were compared between the pigs fed the control and high-fat diets. Stepwise regression was applied to analyze correlations among all these measures, including the previously reported body physical and plasma biochemical variables. RESULTS: The high-fat diet elevated (P < 0.05) plasma concentrations of tumor necrosis factor α, interleukin-6, leptin, and leptin receptor by 29-42% and affected (P < 0.05-0.1) tissue mRNA levels of the selenoprotein and obesity-related genes in 3 patterns. Specifically, the high-fat diet up-regulated 12 selenoprotein genes in 6 tissues, down-regulated 13 selenoprotein genes in 7 tissues, and exerted no effect on 5 genes in any tissue. Body weights and plasma triglyceride concentrations of pigs showed the strongest regressions to tissue mRNA abundances of selenoprotein and obesity-related genes. Among the selenoprotein genes, selenoprotein V and I were ranked as the strongest independent variables for the regression of phenotypic and plasma measures. Meanwhile, agouti signaling protein, adiponectin, and resistin genes represented the strongest independent variables of the obesity-related genes for the regression of tissue selenoprotein mRNA. CONCLUSIONS: The high-fat diet induced inflammation in pigs and affected their gene expression of selenoproteins associated with thioredoxin and oxidoreductase systems, local tissue thyroid hormone activity, endoplasmic reticulum protein degradation, and phosphorylation of lipids. This porcine model may be used to study interactive mechanisms between excess fat intake and selenoprotein function.
BACKGROUND: Relations of the 25 mammalian selenoprotein genes with obesity and the associated inflammation remain unclear. OBJECTIVE: This study explored impacts of high-fat diet-induced obesity on inflammation and expressions of selenoprotein and obesity-related genes in 10 tissues of pigs. METHODS: Plasma and 10 tissues were collected from pigs (n = 10) fed a corn-soy-based control diet or that diet containing 3-7% lard from weanling to finishing (180 d). Plasma concentrations (n = 8) of cytokines and thyroid hormones and tissue mRNA abundance (n = 4) of 25 selenoprotein genes and 16 obesity-related genes were compared between the pigs fed the control and high-fat diets. Stepwise regression was applied to analyze correlations among all these measures, including the previously reported body physical and plasma biochemical variables. RESULTS: The high-fat diet elevated (P < 0.05) plasma concentrations of tumor necrosis factor α, interleukin-6, leptin, and leptin receptor by 29-42% and affected (P < 0.05-0.1) tissue mRNA levels of the selenoprotein and obesity-related genes in 3 patterns. Specifically, the high-fat diet up-regulated 12 selenoprotein genes in 6 tissues, down-regulated 13 selenoprotein genes in 7 tissues, and exerted no effect on 5 genes in any tissue. Body weights and plasma triglyceride concentrations of pigs showed the strongest regressions to tissue mRNA abundances of selenoprotein and obesity-related genes. Among the selenoprotein genes, selenoprotein V and I were ranked as the strongest independent variables for the regression of phenotypic and plasma measures. Meanwhile, agouti signaling protein, adiponectin, and resistin genes represented the strongest independent variables of the obesity-related genes for the regression of tissue selenoprotein mRNA. CONCLUSIONS: The high-fat diet induced inflammation in pigs and affected their gene expression of selenoproteins associated with thioredoxin and oxidoreductase systems, local tissue thyroid hormone activity, endoplasmic reticulum protein degradation, and phosphorylation of lipids. This porcine model may be used to study interactive mechanisms between excess fat intake and selenoprotein function.
Authors: Matthew P Pepper; Marko Z Vatamaniuk; Xi Yan; Carol A Roneker; Xin Gen Lei Journal: Antioxid Redox Signal Date: 2010-09-29 Impact factor: 8.401
Authors: Joanne E Curran; Jeremy B M Jowett; Kate S Elliott; Yuan Gao; Kristi Gluschenko; Jianmin Wang; Dalia M Abel Azim; Guowen Cai; Michael C Mahaney; Anthony G Comuzzie; Thomas D Dyer; Ken R Walder; Paul Zimmet; Jean W MacCluer; Greg R Collier; Ahmed H Kissebah; John Blangero Journal: Nat Genet Date: 2005-10-09 Impact factor: 38.330
Authors: Xinhua Chen; Theresa O Scholl; Maria J Leskiw; Melissa R Donaldson; T Peter Stein Journal: J Clin Endocrinol Metab Date: 2003-12 Impact factor: 5.958
Authors: Kazuko Masuo; Nora E Straznicky; Gavin W Lambert; Tomohiro Katsuya; Ken Sugimoto; Hiromi Rakugi; Florentia Socratous; Jacqueline Hastings; Elisabeth A Lambert; Toshio Ogihara; Murray D Esler Journal: Hypertens Res Date: 2008-06 Impact factor: 3.872