María Luisa Ojeda1, Fátima Nogales2, Alba Membrilla1, Olimpia Carreras1. 1. Department of Physiology, Faculty of Pharmacy, Seville University, C/Professor García González, nº 2, 41012, Seville, Spain. 2. Department of Physiology, Faculty of Pharmacy, Seville University, C/Professor García González, nº 2, 41012, Seville, Spain. fnogales@us.es.
Abstract
PURPOSE: High and low levels of selenium (Se) have been related to metabolic disorders in dams and in their offspring. Their relationship to oxidative balance and to AMP-activated protein kinase (AMPK) is some of the mechanisms proposed. The aim of this study is to acquire information about how Se is involved in metabolic programming. METHODS: Three experimental groups of dam rats were used: control (Se: 0.1 ppm), Se supplemented (Se: 0.5 ppm) and Se deficient (Se: 0.01 ppm). At the end of lactation, the pups' metabolic profile, oxidative balance, Se levels, selenoproteins and IRS-1 hepatic expression, as well as hepatic AMPK activation were measured. RESULTS: The experimental groups present deep changes in Se homeostasis, selenoproteins and IRS-1 hepatic expression, oxidative balance, AMPK activation ratio and insulin levels. They do, however, have different metabolic profiles. CONCLUSIONS: High- and low-Se diets are linked to insulin resistance, yet the mechanisms involved are completely opposite.
PURPOSE: High and low levels of selenium (Se) have been related to metabolic disorders in dams and in their offspring. Their relationship to oxidative balance and to AMP-activated protein kinase (AMPK) is some of the mechanisms proposed. The aim of this study is to acquire information about how Se is involved in metabolic programming. METHODS: Three experimental groups of dam rats were used: control (Se: 0.1 ppm), Se supplemented (Se: 0.5 ppm) and Se deficient (Se: 0.01 ppm). At the end of lactation, the pups' metabolic profile, oxidative balance, Se levels, selenoproteins and IRS-1 hepatic expression, as well as hepatic AMPK activation were measured. RESULTS: The experimental groups present deep changes in Se homeostasis, selenoproteins and IRS-1 hepatic expression, oxidative balance, AMPK activation ratio and insulin levels. They do, however, have different metabolic profiles. CONCLUSIONS: High- and low-Se diets are linked to insulin resistance, yet the mechanisms involved are completely opposite.
Authors: Vyacheslav M Labunskyy; Byung Cheon Lee; Diane E Handy; Joseph Loscalzo; Dolph L Hatfield; Vadim N Gladyshev Journal: Antioxid Redox Signal Date: 2011-03-21 Impact factor: 8.401
Authors: Wellison J S Diniz; Gerd Bobe; Joseph J Klopfenstein; Yunus Gultekin; T Zane Davis; Alison K Ward; Jean A Hall Journal: Genes (Basel) Date: 2021-11-25 Impact factor: 4.096