Eitaro Hiejima1, Tomoki Kawai, Hiroshi Nakase, Tatsuaki Tsuruyama, Takeshi Morimoto, Takahiro Yasumi, Takashi Taga, Hirokazu Kanegane, Masayuki Hori, Katsuyuki Ohmori, Takeshi Higuchi, Minoru Matsuura, Takuya Yoshino, Hiroki Ikeuchi, Kenji Kawada, Yoshiharu Sakai, Mina T Kitazume, Tadakazu Hisamatsu, Tsutomu Chiba, Ryuta Nishikomori, Toshio Heike. 1. *Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan; †Department of Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan; ‡Department of Anatomical, Forensic Medicine, and Pathological Studies, Graduate School of Medicine, Kyoto University, Kyoto, Japan; §Department of Internal Medicine, Division of General Medicine, Hyogo College of Medicine, Hyogo, Japan; ‖Department of Pediatrics, University of Toyama, Toyama, Japan; ¶Department of Pediatrics, Shiga University of Medical Science, Shiga, Japan; **Department of Pediatrics, Tokyo Medical and Dental University, Tokyo, Japan; ††Department of Clinical Laboratory, Kyoto University Hospital, Kyoto, Japan; ‡‡Department of Surgery, Hyogo College of Medicine, Hyogo, Japan; §§Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan; and ‖‖Division of Gastroenterology, Department of Internal Medicine, Keio University School of Medicine, Kyoto, Japan.
Abstract
BACKGROUND: Mucosal-associated invariant T (MAIT) cells are innate-like T cells involved in the homeostasis of mucosal immunity; however, their role in inflammatory bowel disease (IBD) is unclear. METHODS: Flow cytometry was used to enumerate peripheral blood MAIT cells in 88 patients with ulcerative colitis (UC), 68 with Crohn's disease (CD), and in 57 healthy controls. Immunohistochemistry identified MAIT cells in intestinal tissue samples from patients with UC (n = 5) and CD (n = 10), and in control colon (n = 5) and small intestine (n = 9) samples. In addition, expression of activated caspases by MAIT cells in the peripheral blood of 14 patients with UC and 15 patients with CD, and 16 healthy controls was examined. RESULTS: Peripheral blood analysis revealed that patients with IBD had significantly fewer MAIT cells than healthy controls (P < 0.0001). The number of MAIT cells in the inflamed intestinal mucosae of patients with UC and CD was also lower than that in control mucosae (P = 0.0079 and 0.041, respectively). The number of activated caspase-expressing MAIT cells in the peripheral blood of patients with UC and CD was higher than that in healthy controls (P = 0.0061 and 0.0075, respectively), suggesting that the reduced MAIT cell numbers in IBD are associated with an increased level of apoptosis among these cells. CONCLUSIONS: The number of MAIT cells in the peripheral blood and inflamed mucosae of patients with UC and CD was lower than that in non-IBD controls. Also, MAIT cells from patients with IBD exhibited proapoptotic features. These data suggest the pathological involvement and the potential for therapeutic manipulation of these cells in patients with IBD.
BACKGROUND: Mucosal-associated invariant T (MAIT) cells are innate-like T cells involved in the homeostasis of mucosal immunity; however, their role in inflammatory bowel disease (IBD) is unclear. METHODS: Flow cytometry was used to enumerate peripheral blood MAIT cells in 88 patients with ulcerative colitis (UC), 68 with Crohn's disease (CD), and in 57 healthy controls. Immunohistochemistry identified MAIT cells in intestinal tissue samples from patients with UC (n = 5) and CD (n = 10), and in control colon (n = 5) and small intestine (n = 9) samples. In addition, expression of activated caspases by MAIT cells in the peripheral blood of 14 patients with UC and 15 patients with CD, and 16 healthy controls was examined. RESULTS: Peripheral blood analysis revealed that patients with IBD had significantly fewer MAIT cells than healthy controls (P < 0.0001). The number of MAIT cells in the inflamed intestinal mucosae of patients with UC and CD was also lower than that in control mucosae (P = 0.0079 and 0.041, respectively). The number of activated caspase-expressing MAIT cells in the peripheral blood of patients with UC and CD was higher than that in healthy controls (P = 0.0061 and 0.0075, respectively), suggesting that the reduced MAIT cell numbers in IBD are associated with an increased level of apoptosis among these cells. CONCLUSIONS: The number of MAIT cells in the peripheral blood and inflamed mucosae of patients with UC and CD was lower than that in non-IBD controls. Also, MAIT cells from patients with IBD exhibited proapoptotic features. These data suggest the pathological involvement and the potential for therapeutic manipulation of these cells in patients with IBD.
Authors: Erik D Hanson; Eli Danson; William S Evans; William A Wood; Claudio L Battaglini; Samy Sakkal Journal: Med Sci Sports Exerc Date: 2018-09-19 Impact factor: 5.411
Authors: Erik D Hanson; Eli Danson; Catriona V Nguyen-Robertson; Jackson J Fyfe; Nigel K Stepto; David B Bartlett; Samy Sakkal Journal: Eur J Appl Physiol Date: 2017-09-01 Impact factor: 3.078
Authors: Erik D Hanson; Eli Danson; William S Evans; William A Wood; Claudio L Battaglini; Samy Sakkal Journal: Med Sci Sports Exerc Date: 2019-02 Impact factor: 5.411
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