Literature DB >> 25878336

Comparative Evaluation of Two PCR-Based Methods for Detection of Methicillin-Resistant Staphylococcus aureus (MRSA): Xpert MRSA Gen 3 and BD-Max MRSA XT.

Margaux Lepainteur1, Sandrine Delattre2, Sophie Cozza2, Christine Lawrence1, Anne-Laure Roux3, Martin Rottman4.   

Abstract

We compared two walk-away molecular diagnostic assays, the GeneXpert MRSA Gen 3 assay and the BD-Max MRSA XT assay. A total of 119 prospective swabs and 36 culture-positive samples were tested. Xpert MRSA Gen 3 had sensitivity of 95.7% and specificity of 100% versus 87.5% and 97.1% for BD-Max. The difference in agreement with the enriched culture results was significantly in favor of the Xpert assay (P < 0.02, McNemar nonparametric text).
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 25878336      PMCID： PMC4432069          DOI： 10.1128/JCM.03679-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Ward-specific rates of nasal cocolonization with methicillin-susceptible and -resistant Staphylococcus spp. and potential impact on molecular methicillin-resistant Staphylococcus aureus screening tests.

Authors:  Sophie Trouillet-Assant; Jean-Philippe Rasigade; Sebastien Lustig; Yannick Lhoste; Florent Valour; Claude Guerin; Frederic Aubrun; Sylvestre Tigaud; Frederic Laurent
Journal:  J Clin Microbiol       Date:  2013-05-08       Impact factor: 5.948

2.  Comparison of the BD Max methicillin-resistant Staphylococcus aureus (MRSA) assay and the BD GeneOhm MRSA achromopeptidase assay with direct- and enriched-culture techniques using clinical specimens for detection of MRSA.

Authors:  Alexander H Dalpke; Marjeta Hofko; Stefan Zimmermann
Journal:  J Clin Microbiol       Date:  2012-07-18       Impact factor: 5.948

3.  Laboratory evaluation of the BD MAX MRSA assay.

Authors:  Raymond Widen; Vicki Healer; Suzane Silbert
Journal:  J Clin Microbiol       Date:  2014-05-14       Impact factor: 5.948

4.  Evaluation of the BD Max MRSA XT assay for use with different swab types.

Authors:  Alexander H Dalpke; Marjeta Hofko; Christian Stock; Stefan Zimmermann
Journal:  J Clin Microbiol       Date:  2014-09-17       Impact factor: 5.948

5.  Detection and characterisation of SCCmec remnants in multiresistant methicillin-susceptible Staphylococcus aureus causing a clonal outbreak in a Swedish county.

Authors:  M Lindqvist; B Isaksson; C Grub; T Ø Jonassen; A Hällgren
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-05-18       Impact factor: 3.267

6.  First outbreak of community-acquired MRSA USA300 in France: failure to suppress prolonged MRSA carriage despite decontamination procedures.

Authors:  O Baud; S Giron; C Aumeran; D Mouly; G Bardon; M Besson; J Delmas; B Coignard; A Tristan; F Vandenesch; G Illes; O Lesens
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-05-11       Impact factor: 3.267

7.  New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci.

Authors:  A Huletsky; R Giroux; V Rossbach; M Gagnon; M Vaillancourt; M Bernier; F Gagnon; K Truchon; M Bastien; F J Picard; A van Belkum; M Ouellette; P H Roy; M G Bergeron
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

8.  Comparative evaluation of three chromogenic media combined with broth enrichment and the real-time PCR-based Xpert MRSA assay for screening of methicillin-resistant Staphylococcus aureus in nasal swabs.

Authors:  Seungok Lee; Yeon-Joon Park; Kang-Gyun Park; Dong Wook Jekarl; Hyojin Chae; Jin-Kyung Yoo; Sin Won Seo; Jung Eun Choi; Jung Hye Lim; Seon Mi Heo; Ju Hee Seo
Journal:  Ann Lab Med       Date:  2013-06-24       Impact factor: 3.464

9.  Comparative effectiveness of nafcillin or cefazolin versus vancomycin in methicillin-susceptible Staphylococcus aureus bacteremia.

Authors:  Marin L Schweizer; Jon P Furuno; Anthony D Harris; J Kristie Johnson; Michelle D Shardell; Jessina C McGregor; Kerri A Thom; Sara E Cosgrove; George Sakoulas; Eli N Perencevich
Journal:  BMC Infect Dis       Date:  2011-10-19       Impact factor: 3.090

10.  Origin and evolution of European community-acquired methicillin-resistant Staphylococcus aureus.

Authors:  Marc Stegger; Thierry Wirth; Paal S Andersen; Robert L Skov; Anna De Grassi; Patricia Martins Simões; Anne Tristan; Andreas Petersen; Maliha Aziz; Kristoffer Kiil; Ivana Cirković; Edet E Udo; Rosa del Campo; Jaana Vuopio-Varkila; Norazah Ahmad; Sima Tokajian; Georg Peters; Frieder Schaumburg; Barbro Olsson-Liljequist; Michael Givskov; Elizabeth E Driebe; Henrik E Vigh; Adebayo Shittu; Nadjia Ramdani-Bougessa; Jean-Philippe Rasigade; Lance B Price; Francois Vandenesch; Anders R Larsen; Frederic Laurent
Journal:  MBio       Date:  2014-08-26       Impact factor: 7.867
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  5 in total

1.  Evaluation of BD Max StaphSR and BD Max MRSAXT Assays Using ESwab-Collected Specimens.

Authors:  Suzane Silbert; Carly Kubasek; Faris Galambo; Elaine Vendrone; Raymond Widen
Journal:  J Clin Microbiol       Date:  2015-05-27       Impact factor: 5.948

2.  Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay.

Authors:  Karsten Becker; Olivier Denis; Sandrine Roisin; Alexander Mellmann; Evgeny A Idelevich; Dennis Knaack; Sarah van Alen; André Kriegeskorte; Robin Köck; Frieder Schaumburg; Georg Peters; Britta Ballhausen
Journal:  J Clin Microbiol       Date:  2015-10-21       Impact factor: 5.948

Review 3.  Evolving Rapid Methicillin-resistant Staphylococcus aureus Detection: Cover All the Bases.

Authors:  Yamuna Devi Bakthavatchalam; Laura E B Nabarro; Balaji Veeraraghavan
Journal:  J Glob Infect Dis       Date:  2017 Jan-Mar

4.  Characterization of SCCmec Instability in Methicillin-Resistant Staphylococcus aureus Affecting Adjacent Chromosomal Regions, Including the Gene for Staphylococcal Protein A (spa).

Authors:  C R Scharn; I A Tickler; F C Tenover; R V Goering
Journal:  Antimicrob Agents Chemother       Date:  2022-03-07       Impact factor: 5.938

5.  Clinical Relevance of Xpert MRSA/SA in Guiding Therapeutic Decisions for Staphylococcal Infections: A Diagnostic Test Accuracy Analysis.

Authors:  Suvash Chandra Ojha; Ke Chen; Changfeng Sun; Sarfraz Ahmed; Yun-Jian Sheng; Cun-Liang Deng
Journal:  Infect Dis Ther       Date:  2022-04-22
  5 in total

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