Literature DB >> 25868693

How long does it take a static speaking individual to contaminate the immediate environment?

Nicholas J Port1, Victoria L Bowyer, Eleanor A M Graham, Madu S Batuwangala, Guy N Rutty.   

Abstract

Developments in forensic genetic profiling mean that only a very little DNA is required to generate an identifying profile. However, as this sensitivity increases so does the risk of contamination with non-offender DNA, potentially leading to the conviction of innocents, or release of the guilty. The work of Rutty et al. showed that a static and talking person deposited DNA in front of them within a 15-minute period. This work expands on that of Rutty et al. by determining the time period required for an individual to deposit sufficient DNA for a positive identification to be made, and the distance that this contamination can be detected from the speaking individual. To simulate a scene of crime, sheets of Benchkote(®) were used to represent an area of interest and an unprotected subject talked over them for a variety of times, in a variety of positions (standing, kneeling, and sitting at a desk). Results show that contamination by talking in both kneeling and sitting positions occurred almost immediately (<30 seconds, but not from just one sentence) up to 69 cm from the subject. When standing, contamination could be observed up to a maximum 115 cm from the subject, and was only present in one of three repeats when talking for only 30 seconds. This article illustrates how rapidly a static person can potentially contaminate an area in front of him or herself within a laboratory or scene environment, just by talking.

Entities:  

Year:  2006        PMID: 25868693     DOI: 10.1007/s12024-006-0004-z

Source DB:  PubMed          Journal:  Forensic Sci Med Pathol        ISSN: 1547-769X            Impact factor:   2.456


  9 in total

1.  An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA.

Authors:  P Gill; J Whitaker; C Flaxman; N Brown; J Buckleton
Journal:  Forensic Sci Int       Date:  2000-07-24       Impact factor: 2.395

2.  A comparison of the characteristics of profiles produced with the AMPFlSTR SGM Plus multiplex system for both standard and low copy number (LCN) STR DNA analysis.

Authors:  J P Whitaker; E A Cotton; P Gill
Journal:  Forensic Sci Int       Date:  2001-12-01       Impact factor: 2.395

3.  The effectiveness of protective clothing in the reduction of potential DNA contamination of the scene of crime.

Authors:  G N Rutty; A Hopwood; V Tucker
Journal:  Int J Legal Med       Date:  2003-03-22       Impact factor: 2.686

4.  An investigation into the transference and survivability of human DNA following simulated manual strangulation with consideration of the problem of third party contamination.

Authors:  G N Rutty
Journal:  Int J Legal Med       Date:  2002-06       Impact factor: 2.686

5.  Fingerprints as evidence for a genetic profile: morphological study on fingerprints and analysis of exogenous and individual factors affecting DNA typing.

Authors:  Federica Alessandrini; Monia Cecati; Mauro Pesaresi; Chiara Turchi; Flavia Carle; Adriano Tagliabracci
Journal:  J Forensic Sci       Date:  2003-05       Impact factor: 1.832

6.  The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces.

Authors:  Alex Lowe; Caroline Murray; Jonathan Whitaker; Gillian Tully; Peter Gill
Journal:  Forensic Sci Int       Date:  2002-09-10       Impact factor: 2.395

7.  DNA fingerprints from fingerprints.

Authors:  R A van Oorschot; M K Jones
Journal:  Nature       Date:  1997-06-19       Impact factor: 49.962

8.  DNA typing of epithelial cells after strangulation.

Authors:  P Wiegand; M Kleiber
Journal:  Int J Legal Med       Date:  1997       Impact factor: 2.686

9.  An improved method to recover saliva from human skin: the double swab technique.

Authors:  D Sweet; M Lorente; J A Lorente; A Valenzuela; E Villanueva
Journal:  J Forensic Sci       Date:  1997-03       Impact factor: 1.832

  9 in total
  3 in total

1.  Using oral microbial DNA analysis to identify expirated bloodspatter.

Authors:  Andrea E Donaldson; Michael C Taylor; Stephen J Cordiner; Iain L Lamont
Journal:  Int J Legal Med       Date:  2010-02-17       Impact factor: 2.686

Review 2.  DNA reviews: low level DNA profiling.

Authors:  Eleanor A M Graham
Journal:  Forensic Sci Med Pathol       Date:  2008-05-14       Impact factor: 2.007

3.  Defining background DNA levels found on the skin of children aged 0-5 years.

Authors:  Eleanor Alison May Graham; William John Watkins; Frank Dunstan; Sabine Maguire; Diane Nuttall; Chloe Elizabeth Swinfield; Guy Nathan Rutty; Alison M Kemp
Journal:  Int J Legal Med       Date:  2013-08-30       Impact factor: 2.686

  3 in total

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