Julian O Kim1, Sayf Gazala2, Rene Razzak2, Linghong Guo3, Sunita Ghosh3, Wilson H Roa3, Eric L R Bédard4. 1. Department of Oncology, University of Alberta, Edmonton, Alberta, Canada Division of Radiation Oncology, Department of Radiology, University of Manitoba, Winnipeg, Manitoba, Canada Cross Cancer Institute, Edmonton, Alberta, Canada CancerCare, Winnipeg, Manitoba, Canada. 2. Division of Thoracic Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada. 3. Department of Oncology, University of Alberta, Edmonton, Alberta, Canada Cross Cancer Institute, Edmonton, Alberta, Canada. 4. Division of Thoracic Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada elbedard@gmail.com.
Abstract
AIM: To assess if miRNA expression profiling of bronchoalveolar lavage (BAL) fluid and sputum could be used to detect early-stage non-small cell lung cancer (NSCLC). MATERIALS AND METHODS: Hierarchical cluster analysis was performed on the expression levels of 5 miRNAs (miR-21, miR-143, miR-155, miR-210, and miR-372) which were quantified using RNA reverse transcription and quantitative real-time polymerase chain reaction in sputum and BAL samples from NSCLC cases and cancer-free controls. RESULTS: Cluster analysis of the miRNA expression levels in BAL samples from 21 NSCLC cases and sputum samples from 10 cancer-free controls yielded a diagnostic sensitivity of 85.7% and specificity of 100%. Cluster analysis of sputum samples from the same patients yielded a diagnostic sensitivity of 67.8% and specificity of 90%. CONCLUSION: miRNA expression profiling of sputum and BAL fluids represent a potential means to detect early-stage NSCLC. Copyright
AIM: To assess if miRNA expression profiling of bronchoalveolar lavage (BAL) fluid and sputum could be used to detect early-stage non-small cell lung cancer (NSCLC). MATERIALS AND METHODS: Hierarchical cluster analysis was performed on the expression levels of 5 miRNAs (miR-21, miR-143, miR-155, miR-210, and miR-372) which were quantified using RNA reverse transcription and quantitative real-time polymerase chain reaction in sputum and BAL samples from NSCLC cases and cancer-free controls. RESULTS: Cluster analysis of the miRNA expression levels in BAL samples from 21 NSCLC cases and sputum samples from 10 cancer-free controls yielded a diagnostic sensitivity of 85.7% and specificity of 100%. Cluster analysis of sputum samples from the same patients yielded a diagnostic sensitivity of 67.8% and specificity of 90%. CONCLUSION: miRNA expression profiling of sputum and BAL fluids represent a potential means to detect early-stage NSCLC. Copyright
Authors: R Razzak; E L R Bédard; J O Kim; S Gazala; L Guo; S Ghosh; A Joy; T Nijjar; E Wong; W H Roa Journal: Curr Oncol Date: 2016-04-13 Impact factor: 3.677
Authors: Alberto Izzotti; Stefano Carozzo; Alessandra Pulliero; Dinara Zhabayeva; Jean Louis Ravetti; Rakhmet Bersimbaev Journal: Am J Cancer Res Date: 2016-07-01 Impact factor: 6.166
Authors: Erika Larrea; Carla Sole; Lorea Manterola; Ibai Goicoechea; María Armesto; María Arestin; María M Caffarel; Angela M Araujo; María Araiz; Marta Fernandez-Mercado; Charles H Lawrie Journal: Int J Mol Sci Date: 2016-04-27 Impact factor: 5.923