Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.).

Introduction and exploitation of plant growth promoting rhizobacteria (PGPR) in agro-ecosystems enhance plant-microbes interactions that may affect ecosystems sustainability, agricultural productivity, and environmental quality. The present study was conducted to isolate and identify PGPRs associated with maize (Zea mays L.) from twenty sites of Himalayan region of Hajira-Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. A total of 100 isolates were isolated from these sites, out of which eight (HJR1, HJR2, HJR3, HJR4, HJR5, MR6, HJR7, HJR8) were selected in vitro for their plant growth promoting ability (PGPA) including phosphorus solubilization, indole-3-acetic acid (IAA) production and N2 fixation. The 16S rRNA gene sequencing technique was used for molecular identity and authentication. Isolates were then further tested for their effects on growth and nutrient contents of maize (Z. mays L.) under pouch and pot conditions. The 16S rRNA gene sequencing and phylogenetic analysis identified these isolates belong to Pseudomonas and Bacillus genera. The isolates promoted plant growth by solubilizing soil P which ranged between 19.2 and 35.6 μg mL(-1). The isolates HJR1, HJR2, HJR3, and HJR5 showed positive activity in acetylene reduction assay showing their N2-fixation potential. All eight isolates showed the potential to produce IAA in the range of 0.9-5.39 μg mL(-1) and promote plant growth. Results from a subsequent pot experiment indicated PGPRs distinctly increased maize shoot and root length, shoot and root dry weight, root surface area, leaf surface area, shoot and root N and P contents. Among the eight isolates, HR3 showed a marked P-solubilizing activity, plant growth-promoting attributes, and the potential to be developed as a biofertilizers for integrated nutrient management strategies.

Introduction

Intensive farming practices that achieve high yield require continuous application of chemical fertilizers in our agro-ecosystems. However, the prices anpan>d availability of these chemical fertilizers become the limitinpan>g factor for crop productionpan> especially inpan> developinpan>g countries around the world. Conpan>tinpan>uous applicationpan> of N fertilizers may result inpan> negative impacts onpan> agro-ecosystem such as leachinpan>g, pollutionpan> of pan> class="Chemical">water resources, gaseous emissions to atmosphere thus causing irreparable damage to the overall ecosystem and environment. Similarly, phosphorus (P) is one of the major essential macronutrients for biological growth and application of P fertilizers is indispensable component for crop production. However, the availability of P to plants is a serious issue because of its fixation and precipitation behavior in soil which lowers the efficiency of added P. It has been reported that more than 80% of applied P as fertilizers precipitates in the presence of metal ion complexes in soil (Ca2+ in calcareous soils and Fe3+ and Al3+ in acidic soils; Qureshi et al., 2012). In addition to these constraints, the prices of P fertilizers jumped up several folds during recent years making P fertilizers not-affordable to a common resource poor farmer.

Introduction of plant growth promoting rhizobacteria (PGPR) including n class="Chemical">phosphate solubilizinpan>g bacteria (PSB) as biofertilizers is suggested as a sustainpan>able optionpan> for the improvement of nutrient availability, planpan>t growth, anpan>d yields (Vessey, 2003). Use of microbial conpan>sortia inpan> the form of biofertilizers for reducinpan>g the use of chemical fertilizers without compromisinpan>g yield is presently anpan> importanpan>t feature of research inpan> the field of agriculture, microbiology, anpan>d biotechnology (Minpan>orsky, 2008). The search for diverse pan> class="Chemical">PGPRs is gaining serious attention and efforts are made to exploit them as biofertilizers for various economically important crops.

During the last two decades use of microbial techniques and introduction of rhizobacteria in agriculture has increased tremendously due to their potential for n class="Chemical">N2-fixationpan> anpan>d P solubilizationpan> thus inpan>creasinpan>g N anpan>d P uptake by the planpan>ts anpan>d therefore yields (Vessey, 2003). Successful results of usinpan>g PGPR species inpan>cludinpan>g Azospirillum, pan> class="Species">Bacillus, Pseudomonas and Enterobacteria on maize, canola, wheat, and other horticultural crops have been achieved both in the laboratory and in the field under variable ecological conditions (Abbasi et al., 2011; Almaghrabi et al., 2013; Hussain et al., 2013; El-Sayed et al., 2014; Lavakush et al., 2014; Mehta et al., 2014). However, soil–plant–microbe interactions are complex phenomena and detailed explanation had been reported in literature that shows how this interaction influences the plant health and productivity (Mehta et al., 2014). Studies have shown that PGPR strains vary widely and their growth promoting ability may be highly specific to certain plant species, cultivar, soil, and genotype (Lucy et al., 2004). Under such conditions, knowledge of native bacterial population and their identification is important for understanding their distribution and diversity.

It is important to explore and identify region specific microbial strains which can be used as potential plant growth promoters to achieve higher yields under specific ecological and environmental conditions (Fischer et al., 2007). Easy and rapid molecular techniques can be developed to perform microbial characterization. DNA sequences in 16S–23S are known to exhibit a great deal of sequences and length variation which are used to differentiate genera, species up to strain level. Sequence analysis of 16S rRNA gene is more exclusively studied and well-established method for phylogenetic and taxonomic studies (Tan et al., 2001).

Knowledge of the native bacterial population, their characterization and identification is required for understanding the distribution and diversity of indigenous bacteria (Chahboune et al., 2011). With the increasing awareness about the economic and environmental consequences of the use of chemical fertilizers, it is important to explore region specific microbial strains which can be used as a potential plant growth promoter to achieve desired results. The use of indigenous PGPR can be an added advantage since it can easily acclimatize to the natural conditions and enhance the plant–microbe interactions (Verma et al., 2013). Presently, there is very little documentation on the occurrence or utilization of n class="Chemical">PGPRs inpan> the study regionpan>. Therefore, the objectives of this study were (i) to isolate native bacterial strainpan>s from the pan> class="Species">maize (Zea mays L.) rhizosphere under in vitro conditions and to characterize these isolates on the basis of morphological and physiological attributes as well as by 16S rRNA sequence analysis (ii) to assess the PGPAs of these isolates in vivo and their effect on the nutrient contents (N and P) of maize plants at early growth stages.

Materials and Methods

Sample Collection, Bacterial Isolation, and Physiological Characterization

The soil used in this study was collected from twenty different n class="Species">maize growinpan>g sites of Hajira-Rawalakot, AJK, Pakistanpan>. The soil inpan> the study site was Humic Lithic Eutrudepts (Inceptisols). Soil samples were collected from 0 to 20 cm depth at ranpan>dom from five different locationpan>s at each site anpan>d mixed well. The field-moist soil was passed through a 4 mm sieve to eliminpan>ate coarse rock anpan>d planpan>t material, thoroughly mixed to ensure uniformity anpan>d stored pan> class="Species">at 4∘C prior to use. A sub-sample of about 0.5 kg was air- dried and passed through 2-mm sieve and used for the determination of physical and chemical characteristics (Table ). Soil pH was determined in distilled water with a glass electrode (soil:H2O ratio 1:2.5 w/v). Soil total N was determined by the Kjeldahl method (Bremner and Mulvaney, 1982). Soil organic matter was determined using a modified Mebius method (Nelson and Sommers, 1982). Available P from soil samples was determined according to Soil and Plant Analysis Laboratory Manual (Ryan et al., 2001) using AB-DTPA method modified by Soltanpour and Workman (1979). Exchangeable K was determined using a flame photometer following soil extraction with 1 N ammonium acetate (COOCH3NH4; Simard, 1993). The bacterial population was estimated by most probable numbers (PMN) count according to method described by Mirza et al. (2001).

Physico-chemical properties of soil samples collected for isolation studies from different sites of n class="Species">maize growinpan>g areas inpan> Hajira, Rawalakot, Azad Jammu anpan>d Kashmir, Pakistanpan>.

The soil samples were processed at n class="Chemical">Nationpan>al Institute of Biotechnology anpan>d Genetic Enpan>ginpan>eerinpan>g (pan> class="Chemical">NIBGE) Faisalabad, Pakistan for bacterial isolation. For this purpose, plastic pots of about 1 kg capacity were filled with 1 kg soil. Five to six surface sterilized seed of maize (Z. mays L.) were sown in each pot. After 40 days of germination, plants were harvested. Rhizosphere-associated bacteria were isolated by taking 1 g of roots with tightly adhering soil using serial dilution plating technique on LB agar plates (Hameed et al., 2004). The suspension was spread on LB agar plates and incubated at 28 + 2∘C till the appearance of bacterial colonies. Individual colonies were picked and streaked on LB plates for further purification. Differences in cell morphology, i.e., cell shape, motility, acid/alkali production, and gram staining were performed by using phase contrast light microscope as described by Vincent (1970). The ability of the isolates to grow in diverse temperature range was carried out by growing isolates in nutrient broth and incubated at different temperatures ranged from 20 to 40∘C. Growth was recorded every 24 h up-to 96 h. The ability of the isolates to grow in alkaline or acidic media was tested in nutrient agar plates in which the pH was adjusted from 5.0 to 8.0 and incubated at 30∘C for 3 days.

The cell number was calculated from a calibration curve that relates OD values of a series of culture of known cell density. The OD values of cultures containing about ≤0.4 × 109 cell/mL and designated as slow growth (+) ranged from 0.1 to 0.5. The OD values categorized as optimum growth and denoted by (++) ranged from 0.6 to 0.9 while the OD values containing about ≤0.8–2 × cell/mL and designated as maximum growth and is denoted by (+++) ranged ≥1.0 as shown in Table .

Characterization of Bacteria for Plant Growth Promoting Potential

A total of 100 bacterial isolates were screened for their ability to produce n class="Chemical">indole-3-acetic acid (pan> class="Chemical">IAA), phosphate solubilization, and N2 fixation. For IAA production, individual bacterial cultures were grown in LB broth supplemented with tryptophan (100 mg/L) as a precursor of IAA at 28 ± 2∘C with constant shaking (Okon et al., 1977). After 1 week of growth, IAA was extracted from acidified cell-free supernatant using ethyl acetate and analyzed on high-performance liquid chromatograph equipped with Turbochrom software (Perkin Elmer, USA) and C-18 column at a flow rate of 0.5 ml min-1 (Malik et al., 1994). Isolates were categorized into two groups based on their ability to produce IAA in vitro as low IAA producers (1–4 μg mL-1) and medium IAA producers (5–10 μg mL-1) as reported earlier (Khalid et al., 2004). Pure bacterial colonies were inoculated into NFM (Nitrogen Free Malate medium) semisolid medium in vials and incubated at 28 + 2∘C for 48 h. Acetylene (10% v/v) was injected to the vials, incubated at room temperature for 16 h and 100 μL of gas sample from each vial was analyzed on a Gas Chromatograph (Thermoquest, Trace G.C, Model K, Rodono, Milan, Italy) equipped with a Porapak Q column and a H2 -flame ionization detector (FID).

To determine n class="Chemical">phosphate solubilizationpan> ability, each bacterial culture was spot inpan>oculated onpan> Pikovskaya (1948) pan> class="Chemical">agar plates containing tricalcium phosphate as insoluble phosphate source. The plates were incubated at 28 + 2∘C for 7–10 days. Appearance of a clear zone around bacterial colonies indicated the P-solubilization ability of the isolate. Total solubilized phosphate was measured by estimating the available phosphorus in the cell-free supernatant by phospho-molybdate blue color method using spectrophotometer (Halder et al., 1990).

Identification of Potent PGPR based on 16S rRNA Sequencing

Total genomic DNA was extracted by alkaline lysis method (Maniatis et al., 1982). Eubacterial primers fD1 (5′-AGA- GTTTGATCCTGGCTCAG-3′) and rD1 (5′-AAGGAGGTGAT- CCAGCC-3′) which correspond to n class="Species">Escherichia coli 16S rRNA gene were used for PCR amplificationpan> as described by Weisburg et al. (1991). Amplified PCR products were resolved onpan> 1% pan> class="Chemical">agarose gel. Purification of amplified products was done by using PurelinkTM Quick Gel Extraction Kit (Invitrogen) according to manufacturer protocol. The PCR were sequenced commercially by Macrogen (Korea). The gene sequences were compared with others in the GenBank database using the NCBI BLASTn. Multiple sequence alignments were performed by ClustalX and phylogeny was determined by neighbor-joining method (Saitou and Nie, 1987).Tree topology based on re-samplings of 1000 times of the neighbor joining data set was evaluated by boot strap analysis (Felsenstein, 1985).

Plant Inoculation Experiment

Influence of various PGPR isolates on growth and N and P concentration on n class="Species">maize (pan> class="Species">Z. mays L.) plant was examined in pots under greenhouse conditions. Eight PGPR strains isolated from the 100 screened isolates (HJR1, HJR2, HJR3, HJR4, HJR5, MR6, HJR7, and HJR8) along with un-inoculated control and two levels of N and P fertilizer (½toolNP and full NP fertilizer) were selected. The surface sterilized seeds were inoculated by immersion in the PGPR suspension for 1 h. Cleaned earthen pots of 20 cm height and 15 cm depth were used. A combination of about 4 kg soil and 1 kg sand was used for filling in each pot. Four to five air dried surface sterilized seeds were sown in each pot. The experiment was laid down in a completely randomized design with 11 treatments, three replications and total of 33 pots were used in the experiment. Treatments included: (1) HJR1+½toolNP (2) HJR2+½toolNP (3) HJR3+½toolNP (4) HJR4+½toolNP (5) HJR5+½toolNP (6) MR6+½toolNP (7) HJR7+½toolNP (8) HJR8+½toolNP (9) control (without NP and inoculation; 10) ½toolNP (11) Full NP. Nitrogen and P were applied at the rate of 60 and 45 mg kg-1 (full dose) in the form of urea and single super phosphate, respectively. Pots were kept under greenhouse conditions and equally irrigated when needed. The plants were harvested at 30, and 60 days after germination and following measurements were taken. Morphological characteristics and nutrient contents were determined such as shoot and root length, shoot and root dry weight, root surface area (Scanned image analysis program software), leaf surface area (electronic planimeter), N and P contents in shoot and root. The N and P analysis in shoot and root were carried out using the methods described by Winkleman et al. (1990).

Statistical Analysis

The data were subjected to analysis of variance using statistical program (MSTATC, 1990). The differences among various treatment means were compared using the least significant differences test (LSD) n class="Species">at 5% (P≤ 0.05) probability level (Steel anpan>d Torri, 1980).

Results and Discussion

Isolation and Characterization of PGPR

Among the 100 bacterial isolates from the twenty n class="Species">maize growinpan>g sites, eight named as HJR1, HJR2, HJR3, HJR4, HJR5, MR6, HJR7, anpan>d HJR8 were selected based onpan> their ability to produce phytohormonpan>e pan> class="Chemical">IAA, solubilize insoluble phosphate, or fix N2. These bacterial isolates were characterized based on their morphological features such as shape, margins, color, and pigmentation. All eight isolates were fast growing, having round to irregular colony shape with raised elevation and smooth surface. No pigmentation was produced by any of the tested isolate on LB medium (Table ). Isolates HJR4 and HJR5 were small rod; HJR1, HJR2, HJR3, MR6, and HJR7 were medium rods; and HJR8 showed long rods. Except MR6, all isolates were Gram positive. The isolates were grown in ranges of temperature and pH to examine their tolerance to pH and temperature extremes. The results indicated that almost all isolates were able to grow in temperatures ranging between 25 and 40∘C, and pH ranging between 5 and 8, with an optimum temperature of 35∘C and pH of 7.0 (Table ). Our results are similar to those reported for PGPR isolated from apple rhizosphere in Himachal Pradesh, India (Mehta et al., 2014). The apple rhizosphere isolates reported by Mehta et al. (2014) had an optimum growth temperature of 30∘C and pH of 7.0 with similar phenotypic characteristics as observed in our study.

Characterization of Bacterial Isolates

The sequence analysis of 1.5 kb fragment of 16S rRNA gene of all eight bacterial isolates was analyzed by nucleotide Blast analysis. The sequence of isolates HJR1 and HJR3 showed 99% similarity with n class="Species">Bacillus subtilis anpan>d were submitted to GenBanpan>k under accessionpan> number HQ700330 anpan>d HQ700332, respectively. Isolates HJR2, HJR4, anpan>d HJR8 had 99% sequence match with that of pan> class="Species">Bacillus megaterium. These isolates were submitted to GenBank under accession number HQ700331, HQ700333, and HQ700334, respectively. Isolate MR6 showed similarity with genus Pseudomonas and was identified as Pseudomonas stutzeri. These sequences were aligned with sequence of some PGPR of different genera and species within genera from the GenBank database. The phylogenetic tree of these strains constructed by using their 16S rRNA sequences (Figure ) showed that the selected isolates were members of genus Pseudomonas and Bacillus.

Phylogenetic tree of 16S rRNA gene sequences showing the relationships among the isolates isolated from the soils of Himalayan region of Hajira Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan and the related genera. The data of type strains of related species were from GenBank database (the accession numbers are given in parentheses).

n class="Species">Bacillus anpan>d pan> class="Species">Pseudomonas are the most commonly reported genera and represent the dominant isolates in many plant studies (Hallmann and Berg, 2006). The number of isolates belonging to the genus Bacillus was higher in this study similar to those reported earlier explaining that Bacillus spp. are dominant in root adhering soil (Laguerre et al., 1994). Studies on the diversity of root-associated bacteria in maize carried out in different geographical regions, revealed extensive colonization by Bacillus sp. during the active growth stage of the plants (Lambert et al., 1987; Lalande et al., 1989). Both these studies also reported a substantial or rather predominant fraction of Pseudomonas present in the isolation pool (reported in Grönemeyer et al., 2012).

Characterization for Plant Growth Promoting Traits

The isolates associated with the roots of n class="Species">maize crop were tested for features knpan>ownpan> to conpan>tribute to planpan>t growth promotionpan> or bioconpan>trol (Table ).

Production of Indole-3-Acetic Acid

All n class="Species">Bacillus anpan>d pan> class="Species">Pseudomonas spp. produced IAA in vitro in tryptophan supplemented LB medium. Isolates HJR2, HJR7, and HJR8 were medium producers of IAA (4.8–5.3 μg ml-1) while HJR1, HJR4, HJR5, HJR6 designated as weak producers (0.9–2.9 μg ml-1; Table ). The amount of IAA produced by these isolates was substantially lower than that reported earlier from other regions (Fischer et al., 2007; Tahir et al., 2013) but similar to that reported under similar environmental conditions from this region (Abbasi et al., 2011; Zhang et al., 2012). However, it has been reported that the amount of indole compounds produced in vitro depends on the particular bacterial species, strain, or the conditions of the culture such as oxygenation and pH (Radwan et al., 2002). The variation among PGPRs to produce IAA found in the present study had also been reported earlier (Zahir et al., 2000; Abbasi et al., 2011). This variation is attributed to the various biosynthetic pathways, location of the genes involved, regulatory sequences, and the presence of enzymes to convert active free IAA into conjugated forms (Patten and Galick, 1996). A high level of IAA production was recorded in different strains of bacteria with the members of the genera Pseudomonas spp., Bacillus spp., Rhizobium, and Mesorhizobium spp. by other workers (Ahmad et al., 2008; Verma et al., 2012, 2013).

Solubilization of Inorganic Phosphates

The results showed that all isolates had P solubilization potential ranging between 19.2 and 35.6 μg mL-1 (Table ). The highest P solubilization was measured for bacterial isolate HJR3 (35.6 μg mL-1) fallowed by HJR7 (28.9 μg mL-1) and HJR2 (27.4 μg mL-1). The solubilization of n class="Chemical">TCP by different isolates was accompanpan>ied by a signpan>ificanpan>t drop inpan> pH (4.9–3.5) from anpan> inpan>itial pH of 7.0. The maximum drop inpan> culture pH of 3.5 was associated with isolate HJR3. P-solubilizationpan> activity was associated with the release of pan> class="Chemical">organic acids and a drop in pH. Pikovskaya’s medium indicated the efficacy of tested isolates to solubilize the unavailable P (Mehta et al., 2014). It has been reported that P solubilization is mainly due to the production of microbial metabolites including organic acids which decrease the pH of the culture media (Puente et al., 2004; Sahin et al., 2004).

The ability of PGPR strains to solubilize insoluble P and convert it to plant available form is an important characteristic under conditions where P is a limiting factor for crop production. In general, out of the 100 isolates tested, only eight were able to show P solubilization. Such low percentage of isolates that show P solubilization ability is not unique to our study as other studies also show limited numbers. Two separate studies in n class="Species">pearl millet anpan>d pan> class="Species">rice paddy field indicated that only 5% of the 207 total tested strains in of the studies and 23.5% in the other possessed P solubilizing ability (Hameeda et al., 2006; Rashedui et al., 2010). The presence of P-solubilizing microbial population in soils may be considered a positive indicator of utilizing the microbes as biofertilizers for crop production and beneficial for sustainable agriculture.

Based on the n class="Chemical">acetylene reductionpan> assay, isolates HJR1, HJR2, HJR3, anpan>d HJR5 showed the ability to fix pan> class="Chemical">N2. The remaining isolates did not show any potential for N2 fixation (Table ). The presence of N2 fixing bacteria in soil and its isolation and conversion into PGPR biofertilizer is an important strategy reducing the use of expensive chemical fertilizers especially in nutrient poor and degraded soils. Biological N2 fixation provides a major source of N for plants as part of environmental friendly agricultural practices (Cakmakci et al., 2006).

Plant Growth Promotion

Co-inoculation of these isolates along-with 50% reduced fertilizer dose (½toolNP) was tested against un-inoculated/unfertilized control and with two levels of NP fertilizer (½toolNP and full NP fertilizer) to evaluate their potential as PGPR in n class="Species">maize (Table ). Results inpan>dicated that all isolates signpan>ificanpan>tly (P≤ 0.05) inpan>creased the growth of pan> class="Species">maize compared to the control and in some cases to that recorded under ½toolNP treatment. The shoot length, root length, shoot fresh weight, and root fresh weight were highest in HR3+½toolNP and full NP treatments. Root dry weight and leaf area were significantly (P≤ 0.05) higher in HR3+½toolNP compared to the remaining treatments including full NP. Root area was maximum in HR8+½toolNP. Most of the isolates when combined with ½toolNP showed significantly higher growth characteristics compared to the treatments supplemented with ½toolNP. The difference in growth between isolates+½toolNP and ½toolNP treatments was due to the addition of PGPR isolates. The results indicated that the growth traits recorded under HJR3+½toolNP treatment were significantly (P≤ 0.05) higher than those recorded under other isolates, ½toolNP and control treatments but at par with those recoded under full NP fertilizer treatment showing the dominating and promising effect of isolate HJR3 over the remaining isolates (Table ).

Effect of inoculation with plant growth promoting rhizobacteria on the growth of n class="Species">maize (.

Plant growth promotion in response to n class="Chemical">PGPRs applied alonpan>e or with N or P fertilizers has been reported recently for different crops under different ecological anpan>d environpan>mental conpan>ditionpan>s (Lee et al., 2012; Krey et al., 2013; Verma et al., 2013; Lavakush et al., 2014; Mehta et al., 2014). The pan> class="Chemical">PGPRs may promote the plant growth either by using their own metabolism (solubilizing phosphates, producing hormones, or fixing nitrogen) or directly affecting the plant metabolism (increasing the uptake of water and minerals), enhancing root development, increasing the enzymatic activity of the plant or “helping” other beneficial microorganisms to enhance their action on the plants, or by suppressing plant pathogens (Pérez-Montaño et al., 2014). The PGPRs tested in this study possessed multiple plant growth promoting traits including P-solubilization, IAA production, and N2 fixation.

N and P Concentration in Maize Plants

The n class="Chemical">N anpan>d P conpan>tents of both shoot anpan>d root of planpan>ts inpan> responpan>se to PGPR isolates, pan> class="Chemical">NP fertilizer and control treatments is presented in Figure . The N and P contents in both shoot and root showed similar trend in response to different treatments; hence, the N and P contents is presented here as a total of the shoot and root. The total N in plants received the control treatment was 7.4 g kg-1 compared with 14.8–47.7 g kg-1 for plants those received the PGPRs and NP fertilizers, showing a 2–6 fold increase. Among the different amendments, co-inoculation with isolate HJR3+½toolNP displayed superiority over the remaining treatments including full NP treatment. The highest total N content (in shoot+root) of 47.7 g kg-1 was recorded in plants grown under HJR3+½toolNP followed by 34.6, 33.8, and 33.1 g kg-1 N under HJR1+½toolNP, full NP and HJR2+½toolNP treatments, respectively. The relative increase in total N contents under HJR3+½toolNP over HJR1+½toolNP, full NP and HJR2+½toolNP was 38, 41, and 44%, respectively. The N contents observed in HJR3+½toolNP was almost double to those recorded under ½toolNP treatment showing that the additional N in plant was eventually because of isolate HJR3.

Effect of inoculation with n class="Species">Bacillus anpan>d pan> class="Species">Pseudomonas on the shoot and root N and P contents of maize plant grown under greenhouse condition after 60 days. Treatments included T1, HJR1+½toolNP; T2, HJR2+½toolNP; T3, HR3+½toolNP; T4, HJR4+½toolNP, T5, HJR5+½toolNP; T6, MR6+½toolNP; T7, HJR7+½toolNP; T8, HJR8+½toolNP; T9, Control; T10, ½toolNP; T11, Full NP. The vertical lines on each bar represent the standard error of mean (SEM, n = 3).

Total P contents both in shoot and root also showed similar trend as that of total N (Figure ). The minimum total P content of 3.5 and 3.7 g kg-1 was found under the control and ½toolNP fertilizer treatments. Application of different n class="Chemical">PGPRs with ½toolNP signpan>ificanpan>tly inpan>creased planpan>t P conpan>tents to between 7.7 anpan>d 20.6 g kg-1 showinpan>g a 2–6 fold inpan>crease due to inpan>oculationpan> with pan> class="Chemical">PGPRs. The highest P content of 20.6 g kg-1 was recorded under HJR3+½toolNP treatment followed by 17.7 g kg-1 P under HJR2+½toolNP. The full NP fertilizer treatment had 13.7 g kg-1 total P content. The relative increase in P contents in plants grown under HJR3+½toolNP and HJR2+½toolNP over full NP was 50 and 29%, respectively.

These results show the efficient transfer of N and P to plants by the PGPR strains as reported earlier n class="Species">rice (Islam et al., 2009), pan> class="Species">wheat (Abbasi et al., 2011), common bean (Phaseolus vulgaris L.; Tajini et al., 2012), and chickpea (Cicer arietinum L.; Verma et al., 2013). This increase in NP concentration in plant tissues is associated with N2 fixation and P solubilization potential of applied PGPRs. The highest N concentration was recorded in plants supplemented with isolates HJR1, HJR2, HJR3, and HJR5. All of these four isolates showed nitrogenase activity (N2 fixation; Table ) signifying a close association between plant N concentration and N2 fixation. In our previous study, the N content in wheat shoot under control was 1.2% that significantly increased to 1.7–2.43% by the application of different PGPR strains (Abbasi et al., 2011).

The increase in plant P concentration in response to n class="Chemical">PGPRs is attributed to the fact that pan> class="Chemical">PGPRs have the ability to solubilize insoluble phosphates, making it available for plant uptake through different mechanisms such as acidification, chelation, and ion-exchange reactions (Coutinho et al., 2012). The results presented in Table indicate a substantial potential of applied strains to solubilize P and increase P concentration in plants. The increased concentration of N and P in plants supplemented with PGPRs suggest that a positive interaction exists between root colonization, N and P concentration and growth promotion. Further, this study suggests that plant N derived from N2-fixation and P concentration as a result of P-solubilization by phosphate solubilizing microorganism is substantially enhanced above those of uninoculated control plants.

The significant increase in growth and NP level both in shoot and root upon isolates application is a clear indication that bacterial isolates are able to provide better nutrient flux to the plant and result in increased plant biomass. The increase in root length and mass due to the applied isolates may also be a factor that contributes to the increase in N and P concentration in plant shoot and root.

Conclusion

This study indicates the presence of some efficient and effective species of bacteria in the soils of mountainous region of Rawalakot, AJK, Pakistan. Our results demonstrate that efficient n class="Chemical">N2 fixinpan>g anpan>d P solubilizinpan>g isolates are present amonpan>g natural populationpan> of rhizobacteria. These characteristics are importanpan>t growth promotinpan>g traits for planpan>ts growinpan>g inpan> the regionpan> under conpan>tinpan>uous threat of soil erosionpan> anpan>d soil degradationpan>. The combinpan>ationpan> of pan> class="Species">Bacillus spp. with ½toolNP fertilizer resulted in plant growth equivalent to full NP fertilizer treatment while the N and P concentration in plant biomass in this combined treatment was even higher than that recorded under full NP treatment. The effectiveness of PGPR isolates with NP fertilizers clearly indicates that the chemical fertilizers rate or dose could be reduced through combination of PGPR isolates with fertilizers that may be an eco-friendly and cost effective management strategy. These native isolates may be used as efficient bio-inoculants for integrated nutrient management in the uplands soils facing severe threat of erosion and degradation. Therefore, these isolates might have potential in future field applications as plant growth promoters. The future studies should be focused on the functional characterization of PGPR for practical applications in the field.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Table 1

Physico-chemical properties of soil samples collected for isolation studies from different sites of maize growing areas in Hajira, Rawalakot, Azad Jammu and Kashmir, Pakistan.

Location/sites	MPN (cfu g-1)	Organic matter (%)	Total N (%)	Available P (mg kg-1)	pH
Upper Kharange	5.2 × 10-6	1.77	0.2	7.20	7.4
Thanda Gameer	6.4 × 10-6	1.73	0.04	8.15	7.9
Barigalli	7.0 × 10-6	0.38	0.23	7.81	7.8
Numble hut	1.4 × 10-6	1.41	0.27	8.02	7.3
Kalaran	1.8 × 10-8	0.40	0.15	7.68	7.2
Nazar Khawaja	7.1 × 10-8	1.34	0.26	13.48	7.3
Kot Koyan	4.8 × 10-6	0.88	0.04	9.49	7.6
Potha	1.4 × 10-7	1.11	0.06	9.07	7.7
Polus	2 × 10-6	1.24	0.07	8.45	7.4
Chatra	2.2 × 10-8	1.84	0.04	8.86	7.0
Manoria	1.6 × 10-6	1.12	0.08	10.11	8.0
Dawarandi	7.7 × 10-8	1.10	0.03	7.24	7.1
Shar Madarpur	6.0 × 10-8	0.72	0.02	7.81	7.9
Tetrinote	4.3 × 10-6	0.98	0.01	9.04	7.2
Kakuta	8.3 × 10-6	1.76	0.06	8.38	7.6
Buttle	4.1 × 105	0.35	0.04	8.48	7.9
Sehra	3.48 × 18	1.38	0.03	6.45	7.9
Tahi	6.6 × 10-6	0.34	0.04	7.07	8.1
Mandole	6.2 × 10-8	1.26	0.07	9.45	7.6
Hajira	8.2 × 10-8	1.48	0.06	8.56	8.3

Table 2

Colony and cell morphology of selected bacterial isolates and their temperature and pH tolerance ability.

Isolate code	Shape	Margins	Color	Cell shape	Gram s’ reaction	Temperature tolerance (∘C)				pH tolerance
						25	30	35	40	5	6	7	8
HJR1	Round	Smooth	Off white	Medium rod	+ve	+ + +	+ +	+	+	+	+ + +	+ +	+
HJR2	Round	Smooth	Off white	Medium rod	+ve	+	+	+ + +	+	+	+ + +	+ +	+
HJR3	Round	Smooth	Off white	Medium rod	+ve	+	+ + +	+ +	+	+ +	+ + +	+ +	+
HJR4	Round	Smooth	Glossy white	Small rod	+ve	+	+ +	+ + +	+	+	+ + +	+ +	+ +
HJR5	Round	Smooth	Off white	Small rod	+ve	+	+ +	+ + +	+	+ + +	+	+	+
MR6	Round	Smooth	white	Medium rod	-ve	+ + +	+	+	+	+	+	+ + +	+ +
HJR7	Irregular	wavey	Off white	Medium rod	+ve	+	+	+ + +	+	+	+	+ + +	+
HJR8	Round	Smooth	whitish	Long rod	+ve	+	+ + +	+	+	+	+	+ + +	+ +

Table 3

Plant growth promoting potential of different bacterial isolates under .

Isolate code	Identification based on 16S rRNA sequencing	Phosphate solubilization (mg mL-1)	Decrease in pH of medium	IAA production (μg mL-1)	Acetylene reduction assay
HJR1	Bacillus subtilis	25.7	4.2	0.9	+
HJR2	B. megaterium	27.4	4.8	4.8	+
HJR3	B. subtilis	35.6	3.5	2.6	+
HJR4	B. megaterium	22.05	4.5	1.4	–
HJR5	B. megaterium	23.15	4.7	1.9	+
MR6	Pseudomonas stutzeri	19.2	4.9	2.9	–
HJR7	Bacillus sp.	28.9	4.2	5	–
HJR8	B. megaterium	25.7	4.4	5.3	–

Table 4

Effect of inoculation with plant growth promoting rhizobacteria on the growth of maize (.

Treatments	Shoot length (cm)	Root length (cm)	Shoot fresh wt. (g plant-1)	Root fresh wt. (g plant-1)	Shoot dry wt. (g plant-1)	Root dry wt. (g plant-1)	Root area (cm2)	Leaf area (cm2)
HJR1+½toolNP	56.7h	35.6d	20.4gh	19.2e	10.27c	3.6b	228.1e	7.85ab
HJR2+½toolNP	53.4i	19.1h	27.7d	19.3e	7.92de	2.7c	216.4f	4.83d
HJR3+½toolNP	95.2a	44.0a	38.0a	36.1a	18.20a	5.6a	350.5b	8.75a
HJR4+½toolNP	74.1e	23.6g	21.7fg	17.1f	8.39d	2.7c	270.0c	5.33d
HJR5+½toolNP	78.3d	27.5f	23.0ef	19.0e	6.43f	2.0cd	235.4d	4.60de
MR6+½toolNP	61.8g	33.6e	28.7c	17.0f	6.67ef	1.6de	206.4g	3.84e
HJR7+½toolNP	88.0c	41.3bc	34.6b	31.5b	13.40b	2.1cd	221.7f	6.31c
HJR8+½toolNP	86.6c	40.0c	30.0c	24.6d	8.79d	6.0a	406.8a	4.73de
Zero Control	47.9j	16.5i	14.6 j	11.8i	4.13g	1.1e	109.3j	2.20f
½toolNP control	65.8df	28.0cf	22.5d	19.0e	5.50f	1.8de	182.0h	2.04e
Full NP control	93.8a	42.7a	36.8a	34.9a	14.44b	6.3a	136.4j	7.63b
LSD (P ≤ 0.05)	2.60	1.97	1.75	1.81	1.31	0.87	5.88	0.94