| Literature DB >> 25849638 |
Michael W Schmitt1, Edward J Fox2, Marc J Prindle2, Kate S Reid-Bayliss2, Lawrence D True2, Jerald P Radich3, Lawrence A Loeb2.
Abstract
The detection of minority variants in mixed samples requires methods for enrichment and accurate sequencing of small genomic intervals. We describe an efficient approach based on sequential rounds of hybridization with biotinylated oligonucleotides that enables more than 1-million-fold enrichment of genomic regions of interest. In conjunction with error-correcting double-stranded molecular tags, our approach enables the quantification of mutations in individual DNA molecules.Entities:
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Year: 2015 PMID： 25849638 PMCID： PMC4414912 DOI： 10.1038/nmeth.3351
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547