Literature DB >> 25837403

Total internal reflection fluorescence microscopy of intraflagellar transport in Tetrahymena thermophila.

Yu-Yang Jiang1, Karl Lechtreck1, Jacek Gaertig1.   

Abstract

Live imaging has become a powerful tool in studies of ciliary proteins. Tetrahymena thermophila is an established ciliated model with well-developed genetic and biochemical approaches, but its large size, complex shape, and the large number of short and overlapping cilia, have made live imaging of ciliary proteins challenging. Here we describe a method that combines paralysis of cilia by nickel ions and total internal reflection microscopy for live imaging of fluorescent proteins inside cilia of Tetrahymena. Using this method, we quantitatively documented the intraflagellar transport in Tetrahymena.
Copyright © 2015. Published by Elsevier Inc.

Entities:  

Keywords:  Cilia; Ciliate; Dynein; IFT; Kinesin

Mesh：

Substances：

Year:  2015        PMID： 25837403      PMCID： PMC5301313          DOI： 10.1016/bs.mcb.2015.01.001

Source DB:  PubMed          Journal:  Methods Cell Biol        ISSN： 0091-679X            Impact factor:   1.441


  37 in total

1.  Potent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate.

Authors:  I R Gibbons; M P Cosson; J A Evans; B H Gibbons; B Houck; K H Martinson; W S Sale; W J Tang
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

2.  Transformation and strain engineering of Tetrahymena.

Authors:  Douglas L Chalker
Journal:  Methods Cell Biol       Date:  2012       Impact factor: 1.441

Review 3.  Intraflagellar transport (IFT) role in ciliary assembly, resorption and signalling.

Authors:  Lotte B Pedersen; Joel L Rosenbaum
Journal:  Curr Top Dev Biol       Date:  2008       Impact factor: 4.897

4.  Avalanche-like behavior in ciliary import.

Authors:  William B Ludington; Kimberly A Wemmer; Karl F Lechtreck; George B Witman; Wallace F Marshall
Journal:  Proc Natl Acad Sci U S A       Date:  2013-02-19       Impact factor: 11.205

5.  Cilia regeneration in Tetrahymena. A simple reproducible method for producing large numbers of regenerating cells.

Authors:  F J Calzone; M A Gorovsky
Journal:  Exp Cell Res       Date:  1982-08       Impact factor: 3.905

6.  A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila.

Authors:  Yuhua Shang; Xiaoyuan Song; Josephine Bowen; Robert Corstanje; Yan Gao; Jacek Gaertig; Martin A Gorovsky
Journal:  Proc Natl Acad Sci U S A       Date:  2002-03-12       Impact factor: 11.205

7.  Kinesin-II is preferentially targeted to assembling cilia and is required for ciliogenesis and normal cytokinesis in Tetrahymena.

Authors:  J M Brown; C Marsala; R Kosoy; J Gaertig
Journal:  Mol Biol Cell       Date:  1999-10       Impact factor: 4.138

8.  Discovery and functional evaluation of ciliary proteins in Tetrahymena thermophila.

Authors:  Jacek Gaertig; Dorota Wloga; Krishna Kumar Vasudevan; Mayukh Guha; William Dentler
Journal:  Methods Enzymol       Date:  2013       Impact factor: 1.600

9.  Analysis of Ni(2+)-induced arrest of Paramecium axonemes.

Authors:  J Larsen; P Satir
Journal:  J Cell Sci       Date:  1991-05       Impact factor: 5.285

10.  The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly.

Authors:  G J Pazour; B L Dickert; G B Witman
Journal:  J Cell Biol       Date:  1999-02-08       Impact factor: 10.539
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  10 in total

Review 1.  The Intraflagellar Transport Machinery.

Authors:  Michael Taschner; Esben Lorentzen
Journal:  Cold Spring Harb Perspect Biol       Date:  2016-10-03       Impact factor: 10.005

Review 2.  Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery.

Authors:  Bram Prevo; Jonathan M Scholey; Erwin J G Peterman
Journal:  FEBS J       Date:  2017-04-18       Impact factor: 5.542

3.  Methods for Studying Movement of Molecules Within Cilia.

Authors:  Karl F Lechtreck
Journal:  Methods Mol Biol       Date:  2016

4.  Cell Cycle-Related Kinase (CCRK) regulates ciliogenesis and Hedgehog signaling in mice.

Authors:  Ashley Snouffer; Desmond Brown; Hankyu Lee; Jonathon Walsh; Floria Lupu; Ryan Norman; Karl Lechtreck; Hyuk Wan Ko; Jonathan Eggenschwiler
Journal:  PLoS Genet       Date:  2017-08-17       Impact factor: 5.917

5.  Proteins that control the geometry of microtubules at the ends of cilia.

Authors:  Panagiota Louka; Krishna Kumar Vasudevan; Mayukh Guha; Ewa Joachimiak; Dorota Wloga; Raphaël F-X Tomasi; Charles N Baroud; Pascale Dupuis-Williams; Domenico F Galati; Chad G Pearson; Luke M Rice; James J Moresco; John R Yates; Yu-Yang Jiang; Karl Lechtreck; William Dentler; Jacek Gaertig
Journal:  J Cell Biol       Date:  2018-09-14       Impact factor: 10.539

6.  LF4/MOK and a CDK-related kinase regulate the number and length of cilia in Tetrahymena.

Authors:  Yu-Yang Jiang; Wolfgang Maier; Ralf Baumeister; Gregory Minevich; Ewa Joachimiak; Dorota Wloga; Zheng Ruan; Natarajan Kannan; Stephen Bocarro; Anoosh Bahraini; Krishna Kumar Vasudevan; Karl Lechtreck; Eduardo Orias; Jacek Gaertig
Journal:  PLoS Genet       Date:  2019-07-24       Impact factor: 5.917

Review 7.  Motile Cilia: Innovation and Insight From Ciliate Model Organisms.

Authors:  Brian A Bayless; Francesca M Navarro; Mark Winey
Journal:  Front Cell Dev Biol       Date:  2019-11-01

8.  Mutual antagonism between Hippo signaling and cyclin E drives intracellular pattern formation.

Authors:  Yu-Yang Jiang; Wolfgang Maier; Uzoamaka N Chukka; Michael Choromanski; Chinkyu Lee; Ewa Joachimiak; Dorota Wloga; Wayland Yeung; Natarajan Kannan; Joseph Frankel; Jacek Gaertig
Journal:  J Cell Biol       Date:  2020-09-07       Impact factor: 10.539

Review 9.  PCD Genes-From Patients to Model Organisms and Back to Humans.

Authors:  Michal Niziolek; Marta Bicka; Anna Osinka; Zuzanna Samsel; Justyna Sekretarska; Martyna Poprzeczko; Rafal Bazan; Hanna Fabczak; Ewa Joachimiak; Dorota Wloga
Journal:  Int J Mol Sci       Date:  2022-02-03       Impact factor: 5.923

10.  IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium.

Authors:  Jenna L Wingfield; Ilaria Mengoni; Heather Bomberger; Yu-Yang Jiang; Jonathon D Walsh; Jason M Brown; Tyler Picariello; Deborah A Cochran; Bing Zhu; Junmin Pan; Jonathan Eggenschwiler; Jacek Gaertig; George B Witman; Peter Kner; Karl Lechtreck
Journal:  Elife       Date:  2017-05-31       Impact factor: 8.140
  10 in total

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