Literature DB >> 23522474

Discovery and functional evaluation of ciliary proteins in Tetrahymena thermophila.

Jacek Gaertig1, Dorota Wloga, Krishna Kumar Vasudevan, Mayukh Guha, William Dentler.   

Abstract

The ciliate Tetrahymena thermophila is an excellent model system for the discovery and functional studies of ciliary proteins. The power of the model is based on the ease with which cilia can be purified in large quantities for fractionation and proteomic identification, and the ability to knock out any gene by homologous DNA recombination. Here, we include methods used by our laboratories for isolation and fractionation of cilia, in vivo tagging and localization of ciliary proteins, and the evaluation of ciliary mutants.
Copyright © 2013 Elsevier Inc. All rights reserved.

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Year:  2013        PMID： 23522474      PMCID： PMC4392907          DOI： 10.1016/B978-0-12-397944-5.00013-4

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  41 in total

Review 1.  The ciliary transition zone: from morphology and molecules to medicine.

Authors:  Peter G Czarnecki; Jagesh V Shah
Journal:  Trends Cell Biol       Date:  2012-03-06       Impact factor: 20.808

2.  Transformation and strain engineering of Tetrahymena.

Authors:  Douglas L Chalker
Journal:  Methods Cell Biol       Date:  2012       Impact factor: 1.441

3.  Cilia regeneration in Tetrahymena. A simple reproducible method for producing large numbers of regenerating cells.

Authors:  F J Calzone; M A Gorovsky
Journal:  Exp Cell Res       Date:  1982-08       Impact factor: 3.905

4.  Surface display of a parasite antigen in the ciliate Tetrahymena thermophila.

Authors:  J Gaertig; Y Gao; T Tishgarten; T G Clark; H W Dickerson
Journal:  Nat Biotechnol       Date:  1999-05       Impact factor: 54.908

5.  A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila.

Authors:  Yuhua Shang; Xiaoyuan Song; Josephine Bowen; Robert Corstanje; Yan Gao; Jacek Gaertig; Martin A Gorovsky
Journal:  Proc Natl Acad Sci U S A       Date:  2002-03-12       Impact factor: 11.205

6.  Members of the NIMA-related kinase family promote disassembly of cilia by multiple mechanisms.

Authors:  Dorota Wloga; Amy Camba; Krzysztof Rogowski; Gerard Manning; Maria Jerka-Dziadosz; Jacek Gaertig
Journal:  Mol Biol Cell       Date:  2006-04-12       Impact factor: 4.138

7.  Cildb: a knowledgebase for centrosomes and cilia.

Authors:  Olivier Arnaiz; Agata Malinowska; Catherine Klotz; Linda Sperling; Michal Dadlez; France Koll; Jean Cohen
Journal:  Database (Oxford)       Date:  2009-12-07       Impact factor: 3.451

8.  Nonlethal deciliation of Tetrahymena by a local anesthetic and its utility as a tool for studying cilia regeneration.

Authors:  G A Thompson; L C Baugh; L F Walker
Journal:  J Cell Biol       Date:  1974-04       Impact factor: 10.539

9.  Regulation of microtubules in Tetrahymena. I. Electron microscopy of oral replacement.

Authors:  N E Williams; J Frankel
Journal:  J Cell Biol       Date:  1973-02       Impact factor: 10.539

10.  The morphogenesis of basal bodies and accessory structures of the cortex of the ciliated protozoan Tetrahymena pyriformis.

Authors:  R D Allen
Journal:  J Cell Biol       Date:  1969-03       Impact factor: 10.539
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  14 in total

1.  Tetrahymena Poc5 is a transient basal body component that is important for basal body maturation.

Authors:  Westley Heydeck; Brian A Bayless; Alexander J Stemm-Wolf; Eileen T O'Toole; Amy S Fabritius; Courtney Ozzello; Marina Nguyen; Mark Winey
Journal:  J Cell Sci       Date:  2020-06-04       Impact factor: 5.285

2.  Total internal reflection fluorescence microscopy of intraflagellar transport in Tetrahymena thermophila.

Authors:  Yu-Yang Jiang; Karl Lechtreck; Jacek Gaertig
Journal:  Methods Cell Biol       Date:  2015-02-14       Impact factor: 1.441

3.  Two Antagonistic Hippo Signaling Circuits Set the Division Plane at the Medial Position in the Ciliate Tetrahymena.

Authors:  Yu-Yang Jiang; Wolfgang Maier; Ralf Baumeister; Ewa Joachimiak; Zheng Ruan; Natarajan Kannan; Diamond Clarke; Panagiota Louka; Mayukh Guha; Joseph Frankel; Jacek Gaertig
Journal:  Genetics       Date:  2018-12-28       Impact factor: 4.562

4.  Composition and function of the C1b/C1f region in the ciliary central apparatus.

Authors:  Ewa Joachimiak; Anna Osinka; Hanan Farahat; Bianka Świderska; Ewa Sitkiewicz; Martyna Poprzeczko; Hanna Fabczak; Dorota Wloga
Journal:  Sci Rep       Date:  2021-06-03       Impact factor: 4.379

5.  Preparation of Doublet Microtubule Fraction for Single ParticleCryo-electron Microscopy.

Authors:  Corbin Black; Daniel Chen Dai; Katya Peri; Muneyoshi Ichikawa; Khanh Huy Bui
Journal:  Bio Protoc       Date:  2021-06-05

6.  FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c.

Authors:  Krishna Kumar Vasudevan; Kangkang Song; Lea M Alford; Winfield S Sale; Erin E Dymek; Elizabeth F Smith; Todd Hennessey; Ewa Joachimiak; Paulina Urbanska; Dorota Wloga; William Dentler; Daniela Nicastro; Jacek Gaertig
Journal:  Mol Biol Cell       Date:  2014-12-24       Impact factor: 4.138

7.  Kinesin-13 regulates the quantity and quality of tubulin inside cilia.

Authors:  Krishna Kumar Vasudevan; Yu-Yang Jiang; Karl F Lechtreck; Yasuharu Kushida; Lea M Alford; Winfield S Sale; Todd Hennessey; Jacek Gaertig
Journal:  Mol Biol Cell       Date:  2014-12-10       Impact factor: 4.138

8.  The Developmental Process of the Growing Motile Ciliary Tip Region.

Authors:  Matthew J Reynolds; Tanaporn Phetruen; Rebecca L Fisher; Ke Chen; Brian T Pentecost; George Gomez; Puey Ounjai; Haixin Sui
Journal:  Sci Rep       Date:  2018-05-22       Impact factor: 4.379

9.  Proteins that control the geometry of microtubules at the ends of cilia.

Authors:  Panagiota Louka; Krishna Kumar Vasudevan; Mayukh Guha; Ewa Joachimiak; Dorota Wloga; Raphaël F-X Tomasi; Charles N Baroud; Pascale Dupuis-Williams; Domenico F Galati; Chad G Pearson; Luke M Rice; James J Moresco; John R Yates; Yu-Yang Jiang; Karl Lechtreck; William Dentler; Jacek Gaertig
Journal:  J Cell Biol       Date:  2018-09-14       Impact factor: 10.539

Review 10.  Rare Human Diseases: Model Organisms in Deciphering the Molecular Basis of Primary Ciliary Dyskinesia.

Authors:  Martyna Poprzeczko; Marta Bicka; Hanan Farahat; Rafal Bazan; Anna Osinka; Hanna Fabczak; Ewa Joachimiak; Dorota Wloga
Journal:  Cells       Date:  2019-12-11       Impact factor: 6.600
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