| Literature DB >> 25826657 |
Simon Fischer1, René Handrick, Armaz Aschrafi, Kerstin Otte.
Abstract
Understanding the multifaceted nature of microRNA (miRNA) function in mammalian cells is still a challenge. Commonly accepted principles of cooperativity and multiplicity of miRNA function imply that individual mRNAs can be targeted by several miRNAs whereas a single miRNA may concomitantly regulate a subset of different genes. However, there is a paucity of information whether multiple miRNAs regulate critical cellular events and thereby acting redundantly. To gain insight into this notion, we conducted an unbiased high-content miRNA screen by individually introducing 1139 miRNA mimics into Chinese hamster ovary (CHO) cells. We discovered that 66% of all miRNAs significantly impacted on proliferation, protein expression, apoptosis and necrosis. In summary, we provide evidence for a substantial degree of redundancy among miRNAs to maintain cellular homeostasis.Entities:
Keywords: 3'-untranslated region; 3'UTR; AF647; AlexaFluor→647; CH; CHO; CO2; Chinese hamster; Chinese hamster ovary; Cricetulus griseus; MIR; PI; RNA interference; RNAi; SEAP; base pair; bp; cDNA; carbon dioxide; cgr; chinese hamster ovary; mRNA; mature microRNA; miR; miRNA; microRNA; nc; non-coding; pathway regulation; pre-miR; precursor microRNA; propidium iodide; puro; puromycin; qRT-PCR; quantitative reverse-transcriptase real-time PCR; redundancy; rounds per minute; rpm; screen; secreted alkaline phosphatase; siRNA; small-interfering RNA
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Year: 2015 PMID： 25826657 PMCID： PMC4615785 DOI： 10.1080/15476286.2015.1017238
Source DB: PubMed Journal: RNA Biol ISSN： 1547-6286 Impact factor: 4.652