Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Hyperacetylated histones facilitate chromatin assembly in vitro.

Literature DB >> 2582355

Hyperacetylated histones facilitate chromatin assembly in vitro.

Abstract

We have examined the effect of histone acetylation on the in vitro assembly of nucleosomes with DNA and purified histones at physiological ionic strength in the presence of polyglutamic acid. We have found that hyperacetylated histones assemble nucleosomes with greater efficiency, and to a greater extent, than either control or hypoacetylated histones. Assembly reactions were performed over a range of histone to DNA ratios (0.25 to 3.0, w/w) and polyglutamic acid to histone ratios (0 to 1.6, w/w). Although polyglutamic acid may act as a sink to prevent nonspecific histone-DNA interactions, our data suggest that the polyanion primarily facilitates the assembly of nucleosomes by organizing histones into a form that is amenable to deposition.

Entities: Chemical

Mesh：

Substances：

Year: 1985 PMID： 2582355 PMCID： PMC341004 DOI： 10.1093/nar/13.2.401

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

20 in total

Hyperacetylated histones facilitate chromatin assembly in vitro.

1. Processing of newly synthesized histone molecules.

2. The number of superhelical turns in native virion SV40 DNA and minicol DNA determined by the band counting method.

3. Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength.

4. Modification of histones immediately following synthesis.

5. Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.

6. A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.

7. Histones of Drosophila embryos. Electrophoretic isolation and structural studies.

8. Fractionation and characterization of chromosomal proteins by the hydroxyapatite dissociation method.

9. In vitro core particle and nucleosome assembly at physiological ionic strength.

10. Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.

1. Effects of histone acetylation on chromatin topology in vivo.

Review 2. On the biological role of histone acetylation.

3. Influence of core histone acetylation on SV40 minichromosome replication in vitro.

4. Treatment with sodium butyrate inhibits the complete condensation of interphase chromatin.

5. Influence of histone acetylation on the solubility, H1 content and DNase I sensitivity of newly assembled chromatin.

6. Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin.

7. Novobiocin precipitates histones at concentrations normally used to inhibit eukaryotic type II topoisomerase.

8. Novobiocin inhibits passive chromatin assembly in vitro.

9. Purification of a novel, nucleoplasmin-like protein from somatic nuclei.