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Literature DB >> 3412885

Preparation and characterization of biotinylated psoralen.

W A Saffran¹, J T Welsh, R M Knobler, F P Gasparro, C R Cantor, R L Edelson.

Abstract

Biotinylated psoralen (BPsor), a psoralen derivative containing a biotin moiety attached via a long-chain, positively charged linker, has been synthesized and its interactions with DNA and avidin have been studied. As do other psoralen derivatives, BPsor photoreacts with DNA to form interstrand crosslinks. The biotin binds to streptavidin after the reaction of BPsor with DNA, and this property has been used to measure low levels of BPsor modified DNA by ELISA with streptavidin and biotinylated alkaline phosphatase. In addition, BPsor retains the biological activity of psoralen, as shown by its ability to inhibit lymphocyte proliferation at a level of 10 ng/ml.

Entities: Chemical

Mesh：

Substances：

Year: 1988 PMID： 3412885 PMCID： PMC338404 DOI： 10.1093/nar/16.15.7221

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

Review 1. Photochemistry and photobiology of psoralens.

Authors: P S Song; K J Tapley
Journal: Photochem Photobiol Date: 1979-06 Impact factor: 3.421

2. Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light.

Authors: J A Parrish; T B Fitzpatrick; L Tanenbaum; M A Pathak
Journal: N Engl J Med Date: 1974-12-05 Impact factor: 91.245

3. Tumor-associated ganglio-N-triosylceramide. Target for antibody-dependent, avidin-mediated drug killing of tumor cells.

Authors: D L Urdal; S Hakomori
Journal: J Biol Chem Date: 1980-11-10 Impact factor: 5.157

Review 4. Psoralen photochemistry.

Authors: B J Parsons
Journal: Photochem Photobiol Date: 1980-12 Impact factor: 3.421

Preparation and characterization of biotinylated psoralen.

Review 1. Photochemistry and photobiology of psoralens.

2. Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light.

3. Tumor-associated ganglio-N-triosylceramide. Target for antibody-dependent, avidin-mediated drug killing of tumor cells.

Review 4. Psoralen photochemistry.

5. The use of the avidin-biotin complex as a tool in molecular biology.

6. The use of avidin-biotin interaction in immunoenzymatic techniques.

7. UVA-induced inhibition of proliferation of PHA-stimulated lymphocytes from humans treated with 8-methoxypsoralen.

8. Treatment of cutaneous T-cell lymphoma by extracorporeal photochemotherapy. Preliminary results.

9. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

10. Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA.

1. Revised interpretation of oxacillin MICs for Staphylococcus epidermidis based on mecA detection.

2. Transcription forms and remodels supercoiling domains unfolding large-scale chromatin structures.

3. Profiling DNA supercoiling domains in vivo.

Review 4. Investigating DNA supercoiling in eukaryotic genomes.