Literature DB >> 25795469

Model-based strategy for cell culture seed train layout verified at lab scale.

Simon Kern1, Oscar Platas-Barradas2, Ralf Pörtner2, Björn Frahm3.   

Abstract

Cell culture seed trains-the generation of a sufficient viable cell number for the inoculation of the production scale bioreactor, starting from incubator scale-are time- and cost-intensive. Accordingly, a seed train offers potential for optimization regarding its layout and the corresponding proceedings. A tool has been developed to determine the optimal points in time for cell passaging from one scale into the next and it has been applied to two different cell lines at lab scale, AGE1.HN AAT and CHO-K1. For evaluation, experimental seed train realization has been evaluated in comparison to its layout. In case of the AGE1.HN AAT cell line, the results have also been compared to the formerly manually designed seed train. The tool provides the same seed train layout based on the data of only two batches.

Entities:  

Keywords:  Design; Layout; Modeling; Optimization; Seed train; Space–Time–Yield; Suspension cell cultures

Year:  2015        PMID： 25795469      PMCID： PMC4960151          DOI： 10.1007/s10616-015-9858-9

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


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