Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli.

Literature DB >> 2573820

Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli.

Abstract

Efficient in vivo expression of the biodegradative threonine dehydratase (tdc) operon of Escherichia coli is dependent on a regulatory gene, tdcR. The tdcR gene is located 198 base pairs upstream of the tdc operon and is transcribed divergently from this operon. The nucleotide sequence of tdcR and two unrelated reading frames has been determined. The deduced amino acid sequence of TdcR indicates that it is a polypeptide of Mr 12,000 with 99 amino acid residues and contains a potential helix-turn-helix DNA binding motif. Deletion analysis and minicell expression of the tdcR gene suggest that TdcR may serve as a trans-acting positive activator for the tdc operon.

Entities: Gene Species

Mesh：

Substances：

Year: 1989 PMID： 2573820 DOI： 10.1007/BF00332418

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

23 in total

1. Genetic analysis of the tdcABC operon of Escherichia coli K-12.

Authors: H P Schweizer; P Datta
Journal: J Bacteriol Date: 1988-11 Impact factor: 3.490

2. Molecular characterization of the tdc operon of Escherichia coli K-12.

Authors: T J Goss; H P Schweizer; P Datta
Journal: J Bacteriol Date: 1988-11 Impact factor: 3.490

3. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors: H Schägger; G von Jagow
Journal: Anal Biochem Date: 1987-11-01 Impact factor: 3.365

4. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.

Authors: L Wieslander
Journal: Anal Biochem Date: 1979-10-01 Impact factor: 3.365

5. Systematic method for the detection of potential lambda Cro-like DNA-binding regions in proteins.

Authors: I B Dodd; J B Egan
Journal: J Mol Biol Date: 1987-04-05 Impact factor: 5.469

6. Analysis of recombinant DNA using Escherichia coli minicells.

Authors: J E Clark-Curtiss; R Curtiss
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

7. New M13 vectors for cloning.

Authors: J Messing
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

8. Covalent structure of biodegradative threonine dehydratase of Escherichia coli: homology with other dehydratases.

Authors: P Datta; T J Goss; J R Omnaas; R V Patil
Journal: Proc Natl Acad Sci U S A Date: 1987-01 Impact factor: 11.205

Review 9. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.

Authors: H Grosjean; W Fiers
Journal: Gene Date: 1982-06 Impact factor: 3.688

10. Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.

Authors: M Drummond; P Whitty; J Wootton
Journal: EMBO J Date: 1986-02 Impact factor: 11.598

15 in total

8. TdcA, a transcriptional activator of the tdcABC operon of Escherichia coli, is a member of the LysR family of proteins.

Authors: Y L Ganduri; S R Sadda; M W Datta; R K Jambukeswaran; P Datta
Journal: Mol Gen Genet Date: 1993-09

9. Involvement of Fnr and ArcA in anaerobic expression of the tdc operon of Escherichia coli.

Authors: S Chattopadhyay; Y Wu; P Datta
Journal: J Bacteriol Date: 1997-08 Impact factor: 3.490

Review 10. Functions of the gene products of Escherichia coli.

Authors: M Riley
Journal: Microbiol Rev Date: 1993-12

Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli.

1. Genetic analysis of the tdcABC operon of Escherichia coli K-12.

2. Molecular characterization of the tdc operon of Escherichia coli K-12.

3. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

4. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.

5. Systematic method for the detection of potential lambda Cro-like DNA-binding regions in proteins.

6. Analysis of recombinant DNA using Escherichia coli minicells.

7. New M13 vectors for cloning.

8. Covalent structure of biodegradative threonine dehydratase of Escherichia coli: homology with other dehydratases.

Review 9. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.

10. Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.

Review 1. Structure and function of enzymes involved in the anaerobic degradation of L-threonine to propionate.

2. Physical linkage and transcriptional orientation of the tdc operon on the Escherichia coli chromosome.

Review 3. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

4. Integration host factor is required for positive regulation of the tdc operon of Escherichia coli.

5. Physical map location of the tdc operon of Escherichia coli.

6. A novel membrane-associated threonine permease encoded by the tdcC gene of Escherichia coli.

7. Influence of DNA topology on expression of the tdc operon in Escherichia coli K-12.

8. TdcA, a transcriptional activator of the tdcABC operon of Escherichia coli, is a member of the LysR family of proteins.

9. Involvement of Fnr and ArcA in anaerobic expression of the tdc operon of Escherichia coli.

Review 10. Functions of the gene products of Escherichia coli.