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Literature DB >> 25697683

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging.

Amnon Bar-Shir¹, Yajie Liang, Kannie W Y Chan, Assaf A Gilad, Jeff W M Bulte.

Abstract

Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：

Year: 2015 PMID： 25697683 PMCID： PMC4485562 DOI： 10.1039/c4cc10195b

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

22 in total

1. Sensitive NMR detection of cationic-polymer-based gene delivery systems using saturation transfer via proton exchange.

Authors: N Goffeney; J W Bulte; J Duyn; L H Bryant; P C van Zijl
Journal: J Am Chem Soc Date: 2001-09-05 Impact factor: 15.419

2. In vivo magnetic resonance imaging of ferritin-based reporter visualizes native neuroblast migration.

Authors: Bistra Iordanova; Eric T Ahrens
Journal: Neuroimage Date: 2011-09-13 Impact factor: 6.556

3. Supercharging proteins can impart unusual resilience.

Authors: Michael S Lawrence; Kevin J Phillips; David R Liu
Journal: J Am Chem Soc Date: 2007-08-01 Impact factor: 15.419

4. Ovarian carcinoma: quantitative biexponential MR imaging relaxometry reveals the dynamic recruitment of ferritin-expressing fibroblasts to the angiogenic rim of tumors.

Authors: Moriel H Vandsburger; Marina Radoul; Yoseph Addadi; Senzeni Mpofu; Batya Cohen; Raya Eilam; Michal Neeman
Journal: Radiology Date: 2013-06-25 Impact factor: 11.105

5. MRI biosensor for protein kinase A encoded by a single synthetic gene.

Authors: Raag D Airan; Amnon Bar-Shir; Guanshu Liu; Galit Pelled; Michael T McMahon; Peter C M van Zijl; Jeff W M Bulte; Assaf A Gilad
Journal: Magn Reson Med Date: 2012-09-28 Impact factor: 4.668

6. Green fluorescent protein as a marker for gene expression.

Authors: M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
Journal: Science Date: 1994-02-11 Impact factor: 47.728

7. Monitoring enzyme activity using a diamagnetic chemical exchange saturation transfer magnetic resonance imaging contrast agent.

Authors: Guanshu Liu; Yajie Liang; Amnon Bar-Shir; Kannie W Y Chan; Chulani S Galpoththawela; Segun M Bernard; Terence Tse; Nirbhay N Yadav; Piotr Walczak; Michael T McMahon; Jeff W M Bulte; Peter C M van Zijl; Assaf A Gilad
Journal: J Am Chem Soc Date: 2011-09-23 Impact factor: 15.419

8. Chemical exchange saturation transfer (CEST): what is in a name and what isn't?

Authors: Peter C M van Zijl; Nirbhay N Yadav
Journal: Magn Reson Med Date: 2011-02-17 Impact factor: 4.668

9. Transforming thymidine into a magnetic resonance imaging probe for monitoring gene expression.

Authors: Amnon Bar-Shir; Guanshu Liu; Yajie Liang; Nirbhay N Yadav; Michael T McMahon; Piotr Walczak; Sridhar Nimmagadda; Martin G Pomper; Keri A Tallman; Marc M Greenberg; Peter C M van Zijl; Jeff W M Bulte; Assaf A Gilad
Journal: J Am Chem Soc Date: 2013-01-16 Impact factor: 15.419

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging.

1. Sensitive NMR detection of cationic-polymer-based gene delivery systems using saturation transfer via proton exchange.

2. In vivo magnetic resonance imaging of ferritin-based reporter visualizes native neuroblast migration.

3. Supercharging proteins can impart unusual resilience.

4. Ovarian carcinoma: quantitative biexponential MR imaging relaxometry reveals the dynamic recruitment of ferritin-expressing fibroblasts to the angiogenic rim of tumors.

5. MRI biosensor for protein kinase A encoded by a single synthetic gene.

6. Green fluorescent protein as a marker for gene expression.

7. Monitoring enzyme activity using a diamagnetic chemical exchange saturation transfer magnetic resonance imaging contrast agent.

8. Chemical exchange saturation transfer (CEST): what is in a name and what isn't?

9. Transforming thymidine into a magnetic resonance imaging probe for monitoring gene expression.

10. Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins.

Review 1. Genetic engineered molecular imaging probes for applications in cell therapy: emphasis on MRI approach.

Review 2. Cellular and Molecular Imaging Using Chemical Exchange Saturation Transfer.

3. Quantification and tracking of genetically engineered dendritic cells for studying immunotherapy.

4. Carbon Dots as a New Class of Diamagnetic Chemical Exchange Saturation Transfer (diaCEST) MRI Contrast Agents.

5. A Genetically Encoded β-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.

Review 6. Beyond the Green Fluorescent Protein: Biomolecular Reporters for Anaerobic and Deep-Tissue Imaging.

Review 7. New imaging probes to track cell fate: reporter genes in stem cell research.

Review 8. Probing the brain with molecular fMRI.

Review 9. Advances in Magnetic Resonance Imaging Contrast Agents for Biomarker Detection.

Review 10. Clinical Tracking of Cell Transfer and Cell Transplantation: Trials and Tribulations.