| Literature DB >> 25679146 |
Rasa Rakauskaitė1, Giedrė Urbanavičiūtė, Audronė Rukšėnaitė, Zita Liutkevičiūtė, Robertas Juškėnas, Viktoras Masevičius, Saulius Klimašauskas.
Abstract
Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro.Entities:
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Year: 2015 PMID： 25679146 DOI： 10.1039/c4cc07910h
Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222