| Literature DB >> 25677145 |
Guro F Giskeødegård1, Maria D Cao, Tone F Bathen.
Abstract
High-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy is a nondestructive technique that is used to obtain the metabolite profile of a tissue sample. This method requires minimal sample preparation. However, it is important to handle the sample with care and keep it frozen during preparation to minimize degradation. Here, we describe a typical protocol for HR-MAS analysis of intact tissue. We also include examples of typical pulse sequence programs and quantification methods that are used today.Entities:
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Year: 2015 PMID： 25677145 DOI： 10.1007/978-1-4939-2377-9_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745