Shashi Sharma1, Sandeep Saxena1, Khushboo Srivastav1, Rajendra K Shukla2, Nibha Mishra1, Carsten H Meyer3, Peter Kruzliak4, Vinay K Khanna2. 1. Retina Service, Department of Ophthalmology, King George's Medical University, Lucknow, India. 2. Developmental Toxicology Division, Indian Institute of Toxicology and Research, Lucknow, India. 3. Department of Ophthalmology, Pallas Klinik, Olten, Switzerland. 4. Department of Cardiovascular Diseases, International Clinical Research Center, St Anne's University Hospital and Masaryk University, Brno, Czech Republic.
Abstract
BACKGROUND: The aim of the study was to determine plasma nitric oxide (NO) and lipid peroxide (LPO) levels in diabetic retinopathy and its association with severity of disease. DESIGN: Prospective observational study. PARTICIPANTS: A total of 60 consecutive cases and 20 healthy controls were included. METHODS: Severity of retinopathy was graded according to early treatment diabetic retinopathy study (ETDRS) classification. Photoreceptor inner segment ellipsoid band (ISel) disruption and retinal pigment epithelium (RPE) alteration were graded using spectral domain optical coherence tomography. Data were statistically analyzed. MAIN OUTCOME MEASURES: Plasma thiobarbituric acid reactive substances, NO assay and reduced glutathione (GSH) were measured using standard protocol. RESULTS: Increased severity of diabetic retinopathy was significantly associated with increase in plasma levels of LPO (P < 0.05), NO (P < 0.001) and decrease in plasma levels of GSH (P < 0.0001), ISel disruption (P < 0.001) and RPE topographic alteration (P < 0.01). CONCLUSION: Increased plasma NO levels are associated with increased severity of diabetic retinopathy. For the first time, it has been demonstrated that increased plasma LPO, NO and decreased GSH levels are associated with in vivo structural changes in inner segment ellipsoid and RPE.
BACKGROUND: The aim of the study was to determine plasma nitric oxide (NO) and lipid peroxide (LPO) levels in diabetic retinopathy and its association with severity of disease. DESIGN: Prospective observational study. PARTICIPANTS: A total of 60 consecutive cases and 20 healthy controls were included. METHODS: Severity of retinopathy was graded according to early treatment diabetic retinopathy study (ETDRS) classification. Photoreceptor inner segment ellipsoid band (ISel) disruption and retinal pigment epithelium (RPE) alteration were graded using spectral domain optical coherence tomography. Data were statistically analyzed. MAIN OUTCOME MEASURES: Plasma thiobarbituric acid reactive substances, NO assay and reduced glutathione (GSH) were measured using standard protocol. RESULTS: Increased severity of diabetic retinopathy was significantly associated with increase in plasma levels of LPO (P < 0.05), NO (P < 0.001) and decrease in plasma levels of GSH (P < 0.0001), ISel disruption (P < 0.001) and RPE topographic alteration (P < 0.01). CONCLUSION: Increased plasma NO levels are associated with increased severity of diabetic retinopathy. For the first time, it has been demonstrated that increased plasma LPO, NO and decreased GSH levels are associated with in vivo structural changes in inner segment ellipsoid and RPE.
Authors: Jana Stefanickova; Sandeep Saxena; Dwividendra K Nim; Kaleem Ahmad; Abbas A Mahdi; Apjit Kaur; Shashi K Bhasker; Jela Valaskova; Peter Kruzliak Journal: Int Ophthalmol Date: 2019-01-09 Impact factor: 2.031
Authors: Kiu Kwong-Han; Embong Zunaina; Hashim Hanizasurana; Abd Aziz Che-Badariah; Che Hussin Che-Maraina Journal: J Diabetes Metab Disord Date: 2022-03-19
Authors: Zsuzsanna Géhl; Edina Bakondi; Miklós D Resch; Csaba Hegedűs; Katalin Kovács; Petra Lakatos; Antal Szabó; Zoltán Nagy; László Virág Journal: Redox Biol Date: 2016-07-08 Impact factor: 11.799