Mohammed S Alkatheeri1, Jadesada Palasuk1,2, George J Eckert3, Jeffrey A Platt1, Marco C Bottino4. 1. Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), 1121 W. Michigan Street, Indianapolis, IN, 46202, USA. 2. Department of Restorative Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand. 3. Department of Biostatistics, Indiana University School of Medicine, Indianapolis, IN, 46202, USA. 4. Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), 1121 W. Michigan Street, Indianapolis, IN, 46202, USA. mbottino@iu.edu.
Abstract
OBJECTIVES: This study aimed to evaluate the effect of Halloysite® aluminosilicate clay nanotube (HNT) incorporation into a two-step etch-and-rinse (ER) and a one-step self-etch (SE) adhesive on human dentin shear bond strength (SBS). MATERIALS AND METHODS: Ten groups (n = 12) were prepared according to the adhesive system (i.e., ER or SE) and amount of HNT incorporated (5-20%, w/v), as follows: commercial control (i.e., the adhesive was used as purchased, 0% HNT); experimental control (i.e., the adhesive was processed through mixing/stirring and sonication similarly to the HNT-incorporated experimental groups, but without HNT); and 5, 10, and 20% HNT. SBS testing was performed after 24 h of storage in deionized water at 37 °C. Failure modes were examined using a stereomicroscope (×40). Scanning electron microscopy (SEM) of the resin-dentin interface of selected specimens was carried out. RESULTS: Two-way ANOVA revealed that incorporation of HNT up to 20% (w/v) in ER and up to 10% (w/v) in SE demonstrated an increased SBS compared to their experimental controls. Compared to the commercial control, SBS of HNT-modified dentin adhesives was not significantly different for ER adhesives (p > 0.05) but was significantly higher with 5% HNT in the SE adhesive (p < 0.05). Failure modes were predominantly adhesive and mixed failures. SEM micrographs of resin-dentin interfaces for ER-commercial control and ER-10% showed a similar morphology. A thicker adhesive layer and the presence of agglomerated HNT on the resin tags were seen in ER-10%. An increased number of short resin tags in SE-5% compared with SE-commercial control were observed. CONCLUSIONS: HNT addition up to 20% in ER and up to 10 % in SE showed increased SBS to dentin compared with the experimental control. CLINICAL RELEVANCE: HNT can be used not only to reinforce adhesive resins but also hold potential for the development of bioactive adhesives by the encapsulation of matrix metalloproteinase (MMP) inhibitors or anticariogenic agents.
OBJECTIVES: This study aimed to evaluate the effect of Halloysite® aluminosilicate clay nanotube (HNT) incorporation into a two-step etch-and-rinse (ER) and a one-step self-etch (SE) adhesive on human dentin shear bond strength (SBS). MATERIALS AND METHODS: Ten groups (n = 12) were prepared according to the adhesive system (i.e., ER or SE) and amount of HNT incorporated (5-20%, w/v), as follows: commercial control (i.e., the adhesive was used as purchased, 0% HNT); experimental control (i.e., the adhesive was processed through mixing/stirring and sonication similarly to the HNT-incorporated experimental groups, but without HNT); and 5, 10, and 20% HNT. SBS testing was performed after 24 h of storage in deionized water at 37 °C. Failure modes were examined using a stereomicroscope (×40). Scanning electron microscopy (SEM) of the resin-dentin interface of selected specimens was carried out. RESULTS: Two-way ANOVA revealed that incorporation of HNT up to 20% (w/v) in ER and up to 10% (w/v) in SE demonstrated an increased SBS compared to their experimental controls. Compared to the commercial control, SBS of HNT-modified dentin adhesives was not significantly different for ER adhesives (p > 0.05) but was significantly higher with 5% HNT in the SE adhesive (p < 0.05). Failure modes were predominantly adhesive and mixed failures. SEM micrographs of resin-dentin interfaces for ER-commercial control and ER-10% showed a similar morphology. A thicker adhesive layer and the presence of agglomerated HNT on the resin tags were seen in ER-10%. An increased number of short resin tags in SE-5% compared with SE-commercial control were observed. CONCLUSIONS: HNT addition up to 20% in ER and up to 10 % in SE showed increased SBS to dentin compared with the experimental control. CLINICAL RELEVANCE: HNT can be used not only to reinforce adhesive resins but also hold potential for the development of bioactive adhesives by the encapsulation of matrix metalloproteinase (MMP) inhibitors or anticariogenic agents.
Entities:
Keywords:
Adhesive; Bond strength; Dentin; Halloysite; Nanotube; SEM
Authors: Marco C Bottino; Ghaeth H Yassen; Jeffrey A Platt; Nawaf Labban; L Jack Windsor; Kenneth J Spolnik; Ana H A Bressiani Journal: J Tissue Eng Regen Med Date: 2013-03-08 Impact factor: 3.963
Authors: S A Feitosa; J Palasuk; K Kamocki; S Geraldeli; R L Gregory; J A Platt; L J Windsor; M C Bottino Journal: J Dent Res Date: 2014-09-08 Impact factor: 6.116
Authors: Sabrina A Feitosa; Jadesada Palasuk; Saulo Geraldeli; Lester Jack Windsor; Marco C Bottino Journal: J Biomed Mater Res B Appl Biomater Date: 2018-09-10 Impact factor: 3.368
Authors: Sara Kalagi; Sabrina A Feitosa; Eliseu A Münchow; Victor M Martins; Ashley E Karczewski; N Blaine Cook; Kim Diefenderfer; George J Eckert; Saulo Geraldeli; Marco C Bottino Journal: Dent Mater Date: 2020-03-30 Impact factor: 5.304
Authors: Jadesada Palasuk; L Jack Windsor; Jeffrey A Platt; Yuri Lvov; Saulo Geraldeli; Marco C Bottino Journal: Clin Oral Investig Date: 2017-09-30 Impact factor: 3.573
Authors: Diana A Cunha; Nara S Rodrigues; Lidiane C Souza; Diego Lomonaco; Flávia P Rodrigues; Felipe W Degrazia; Fabrício M Collares; Salvatore Sauro; Vicente P A Saboia Journal: Materials (Basel) Date: 2018-06-25 Impact factor: 3.623