Jadesada Palasuk1,2, L Jack Windsor2, Jeffrey A Platt2, Yuri Lvov3, Saulo Geraldeli4, Marco C Bottino5. 1. Department of Restorative Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000, Thailand. 2. Department of Biomedical and Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN, 46202, USA. 3. Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA, 71272, USA. 4. Department of Restorative Dental Sciences, Operative Division, College of Dentistry, University of Florida, Gainesville, FL, 32610, USA. 5. Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N. University Avenue, Ann Arbor, MI, 48109, USA. mbottino@umich.edu.
Abstract
OBJECTIVES: This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration. MATERIALS AND METHODS: Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated. RESULTS: Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly, it did inhibit MMP-1 activity. CONCLUSIONS: The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity. CLINICAL SIGNIFICANCE: Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability.
OBJECTIVES: This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration. MATERIALS AND METHODS: Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated. RESULTS: Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly, it did inhibit MMP-1 activity. CONCLUSIONS: The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity. CLINICAL SIGNIFICANCE: Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability.
Authors: M Centlivre; X Zhou; S M Pouw; K Weijer; W Kleibeuker; A T Das; B Blom; J Seppen; B Berkhout; N Legrand Journal: Gene Ther Date: 2009-09-03 Impact factor: 5.250
Authors: A Mazzoni; L Tjäderhane; V Checchi; R Di Lenarda; T Salo; F R Tay; D H Pashley; L Breschi Journal: J Dent Res Date: 2014-12-22 Impact factor: 6.116
Authors: Sabrina A Feitosa; Jadesada Palasuk; Saulo Geraldeli; Lester Jack Windsor; Marco C Bottino Journal: J Biomed Mater Res B Appl Biomater Date: 2018-09-10 Impact factor: 3.368
Authors: Juliana S Ribeiro; Ester A F Bordini; Jessica A Ferreira; Ling Mei; Nileshkumar Dubey; J Christopher Fenno; Evandro Piva; Rafael G Lund; Anna Schwendeman; Marco C Bottino Journal: ACS Appl Mater Interfaces Date: 2020-03-25 Impact factor: 9.229
Authors: Diana A Cunha; Nara S Rodrigues; Lidiane C Souza; Diego Lomonaco; Flávia P Rodrigues; Felipe W Degrazia; Fabrício M Collares; Salvatore Sauro; Vicente P A Saboia Journal: Materials (Basel) Date: 2018-06-25 Impact factor: 3.623
Authors: Manuel Toledano-Osorio; Jegdish P Babu; Raquel Osorio; Antonio L Medina-Castillo; Franklin García-Godoy; Manuel Toledano Journal: Materials (Basel) Date: 2018-06-14 Impact factor: 3.623
Authors: Argyris C Hadjimichael; Athanasios F Foukas; Olga D Savvidou; Andreas F Mavrogenis; Amanda K Psyrri; Panayiotis J Papagelopoulos Journal: Clin Sarcoma Res Date: 2020-04-30