Alba Moratalla1,2, Isabel Gómez-Hurtado1, Ángela Moya-Pérez3, Pedro Zapater1,4, Gloria Peiró5, José M González-Navajas1,2, Eva Maria Gómez Del Pulgar3, José Such1,6, Yolanda Sanz3, Rubén Francés7,8,9. 1. CIBERehd, Instituto de Salud Carlos III, Madrid, Spain. 2. FISABIO, Hospital General Universitario de Alicante, Alicante, Spain. 3. Grupo Ecología Microbiana, Nutrición y Salud, IATA-CSIC, Valencia, Spain. 4. Servicio de Farmacología Clínica, Hospital General Universitario de Alicante, Alicante, Spain. 5. Servicio de Anatomía Patológica, Hospital General Universitario de Alicante, Alicante, Spain. 6. Departamento de Medicina Clínica, Universidad Miguel Hernández, San Juan, Spain. 7. CIBERehd, Instituto de Salud Carlos III, Madrid, Spain. frances_rub@gva.es. 8. FISABIO, Hospital General Universitario de Alicante, Alicante, Spain. frances_rub@gva.es. 9. Departamento de Medicina Clínica, Universidad Miguel Hernández, San Juan, Spain. frances_rub@gva.es.
Abstract
BACKGROUND: Intestinal homeostasis plays an important role in bacteria-derived complications in cirrhosis. Intestinal lymphocytes are responsible for immune effector functions and can be modulated by certain probiotics. We evaluate the interaction between Bifidobacterium pseudocatenulatum CECT7765 and intestinal lymphocytes in mice with cirrhosis. ANIMALS AND METHODS: Cirrhosis was induced by intragastrical administration of carbon tetrachloride in Balb/C mice. One week prior to laparotomy, animals received B. pseudocatenulatum CECT7765 (10(7), 10(9) or 10(10) cfu/daily) or placebo. Chemokine receptor and cytokine expression were evaluated in intestinal lymphocytes. Gut permeability was studied by FITC-LPS recovery in vivo. Luminal antigens, inflammation and functional markers were evaluated in liver samples. RESULTS: Bifidobacterium pseudocatenulatum CECT7765 decreased the expression of pro-inflammatory chemokine receptors CCR6, CCR9, CXCR3 and CXCR6 in intestinal lymphocytes from cirrhotic mice in a concentration-dependent manner. The bifidobacterial strain induced a shift towards an anti-inflammatory cytokine profile in this cell subset. B. pseudocatenulatum CECT7765-induced inflammatory modulation was TLR2-mediated, as in vitro TLR2 blockade inhibited the reduction of TNF-alpha and its receptors and the increase of IL-10 and IL-10 receptor secretion. The recovery rate of administered fluorescence-labelled endotoxin was significantly and dose-dependently lowered with the bifidobacterial strain. The reduced intestinal permeability was associated with a decreased burden of bacterial antigens in the liver of mice treated with B. pseudocatenulatum CECT7765. Liver function and inflammation were improved with the use of the bifidobacterial strain at the highest dose tested (10(10) cfu). CONCLUSION: Bifidobacterium pseudocatenulatum CECT7765 improves gut homeostasis and prevents gut-derived complications in experimental chronic liver disease.
BACKGROUND: Intestinal homeostasis plays an important role in bacteria-derived complications in cirrhosis. Intestinal lymphocytes are responsible for immune effector functions and can be modulated by certain probiotics. We evaluate the interaction between Bifidobacterium pseudocatenulatumCECT7765 and intestinal lymphocytes in mice with cirrhosis. ANIMALS AND METHODS: Cirrhosis was induced by intragastrical administration of carbon tetrachloride in Balb/C mice. One week prior to laparotomy, animals received B. pseudocatenulatumCECT7765 (10(7), 10(9) or 10(10) cfu/daily) or placebo. Chemokine receptor and cytokine expression were evaluated in intestinal lymphocytes. Gut permeability was studied by FITC-LPS recovery in vivo. Luminal antigens, inflammation and functional markers were evaluated in liver samples. RESULTS:Bifidobacterium pseudocatenulatumCECT7765 decreased the expression of pro-inflammatory chemokine receptors CCR6, CCR9, CXCR3 and CXCR6 in intestinal lymphocytes from cirrhotic mice in a concentration-dependent manner. The bifidobacterial strain induced a shift towards an anti-inflammatory cytokine profile in this cell subset. B. pseudocatenulatumCECT7765-induced inflammatory modulation was TLR2-mediated, as in vitro TLR2 blockade inhibited the reduction of TNF-alpha and its receptors and the increase of IL-10 and IL-10 receptor secretion. The recovery rate of administered fluorescence-labelled endotoxin was significantly and dose-dependently lowered with the bifidobacterial strain. The reduced intestinal permeability was associated with a decreased burden of bacterial antigens in the liver of mice treated with B. pseudocatenulatumCECT7765. Liver function and inflammation were improved with the use of the bifidobacterial strain at the highest dose tested (10(10) cfu). CONCLUSION:Bifidobacterium pseudocatenulatumCECT7765 improves gut homeostasis and prevents gut-derived complications in experimental chronic liver disease.
Authors: Hanna Stenstad; Anna Ericsson; Bengt Johansson-Lindbom; Marcus Svensson; Jan Marsal; Matthias Mack; Dominic Picarella; Dulce Soler; Gabriel Marquez; Mike Briskin; William W Agace Journal: Blood Date: 2006-01-03 Impact factor: 22.113
Authors: Theo S Plantinga; Wendy W C van Maren; Jeroen van Bergenhenegouwen; Marjolijn Hameetman; Stefan Nierkens; Cor Jacobs; Dirk J de Jong; Leo A B Joosten; Belinda van't Land; Johan Garssen; Gosse J Adema; Mihai G Netea Journal: Clin Vaccine Immunol Date: 2011-02-02
Authors: José Such; Rubén Francés; Carlos Muñoz; Pedro Zapater; Juan A Casellas; Ana Cifuentes; Francisco Rodríguez-Valera; Sonia Pascual; Javier Sola-Vera; Fernando Carnicer; Francisco Uceda; José M Palazón; Miguel Pérez-Mateo Journal: Hepatology Date: 2002-07 Impact factor: 17.425
Authors: Fraser L Collins; Naiomy Deliz Rios-Arce; Jonathan D Schepper; A Daniel Jones; Laura Schaefer; Robert A Britton; Laura R McCabe; Narayanan Parameswaran Journal: Sci Rep Date: 2019-10-11 Impact factor: 4.379
Authors: Inmaculada López-Almela; Marina Romaní-Pérez; Clara Bullich-Vilarrubias; Alfonso Benítez-Páez; Eva M Gómez Del Pulgar; Rubén Francés; Gerhard Liebisch; Yolanda Sanz Journal: Gut Microbes Date: 2021 Jan-Dec
Authors: Hao Chung The; Paola Florez de Sessions; Song Jie; Duy Pham Thanh; Corinne N Thompson; Chau Nguyen Ngoc Minh; Collins Wenhan Chu; Tuan-Anh Tran; Nicholas R Thomson; Guy E Thwaites; Maia A Rabaa; Martin Hibberd; Stephen Baker Journal: Gut Microbes Date: 2017-08-24