Literature DB >> 2564732

Mapping of recombinants near the Huntington disease locus by using G8 (D4S10) and newly isolated markers in the D4S10 region.

M I Skraastad1, E Bakker, L F de Lange, M Vegter-van der Vlis, E G Klein-Breteler, G J van Ommen, P L Pearson.   

Abstract

Genetic linkage between the marker G8 (D4S10) and Huntington disease (HD) was studied in six Dutch pedigrees. The informativeness of the D4S10 locus was increased by isolation of a cosmid, C5.5, with a G8 subclone used as probe. We present a restriction map of 70 kb in the D4S10 region. Two subclones of C5.5, H5.52 and F5.53, detect MspI and SinI RFLPs, respectively. These probes increase the informativeness of D4S10 in the Dutch HD population from 55% to 95%. Seven recombinations were found in 124 informative meioses in which multipoint segregation of D4S10 haplotypes and the HD locus was studied. Two of the recombinations occurred within the D4S10 region. The other five recombinations are highly valuable for the mapping of present and future markers relative to each other and to the HD gene. In addition, several recombinations between markers in meioses from unaffected parents were noted, which will also be useful in ordering new markers. On the basis of our three-point recombination data, the orientation of the D4S10 region relative to HD is HD-H5.52-G8-F5.53, which independently confirms the previously derived polarity for D4S10.

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Year:  1989        PMID： 2564732      PMCID： PMC1715596     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  20 in total

1.  Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.

Authors:  F S Collins; S M Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

2.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

3.  Genomic sequencing.

Authors:  G M Church; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

4.  Double cos site vectors: simplified cosmid cloning.

Authors:  P F Bates; R A Swift
Journal:  Gene       Date:  1983-12       Impact factor: 3.688

5.  The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end.

Authors:  G J van Ommen; A C Arnberg; F Baas; H Brocas; A Sterk; W H Tegelaers; G Vassart; J J de Vijlder
Journal:  Nucleic Acids Res       Date:  1983-04-25       Impact factor: 16.971

6.  Isolation of probes detecting restriction fragment length polymorphisms from X chromosome-specific libraries: potential use for diagnosis of Duchenne muscular dystrophy.

Authors:  M H Hofker; M C Wapenaar; N Goor; E Bakker; G J van Ommen; P L Pearson
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

7.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

Review 8.  Huntington disease: genetics and epidemiology.

Authors:  P M Conneally
Journal:  Am J Hum Genet       Date:  1984-05       Impact factor: 11.025

9.  Anthropometric discrimination among affected, at-risk, and not-at-risk individuals in families with Huntington disease.

Authors:  L A Farrer; P L Yu
Journal:  Am J Med Genet       Date:  1985-06

10.  Construction of a NotI linking library and isolation of new markers close to the Huntington's disease gene.

Authors:  T M Pohl; M Zimmer; M E MacDonald; B Smith; M Bucan; A Poustka; S Volinia; S Searle; G Zehetner; J J Wasmuth
Journal:  Nucleic Acids Res       Date:  1988-10-11       Impact factor: 16.971
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  7 in total

1.  The end in sight for Huntington disease?

Authors:  C Pritchard; D R Cox; R M Myers
Journal:  Am J Hum Genet       Date:  1991-07       Impact factor: 11.025

2.  Defining DNA diagnostic tests appropriate or standard clinical care.

Authors:  R V Lebo; G Cunningham; M J Simons; L J Shapiro
Journal:  Am J Hum Genet       Date:  1990-09       Impact factor: 11.025

3.  Molecular analysis of 4p deletion associated with Wolf-Hirschhorn syndrome moving the "critical segment" towards the telomere.

Authors:  M Anvret; M Nordenskjöld; L Stolpe; L Johansson; K Bröndum-Nielsen
Journal:  Hum Genet       Date:  1991-03       Impact factor: 4.132

4.  Defined physical limits of the Huntington disease gene candidate region.

Authors:  G P Bates; M E MacDonald; S Baxendale; S Youngman; C Lin; W L Whaley; J J Wasmuth; J F Gusella; H Lehrach
Journal:  Am J Hum Genet       Date:  1991-07       Impact factor: 11.025

5.  Dynamic mutation in Dutch Huntington's disease patients: increased paternal repeat instability extending to within the normal size range.

Authors:  K E De Rooij; P A De Koning Gans; M I Skraastad; R D Belfroid; M Vegter-Van Der Vlis; R A Roos; E Bakker; G J Van Ommen; J T Den Dunnen; M Losekoot
Journal:  J Med Genet       Date:  1993-12       Impact factor: 6.318

6.  Significant linkage disequilibrium between the Huntington disease gene and the loci D4S10 and D4S95 in the Dutch population.

Authors:  M I Skraastad; E Van de Vosse; R Belfroid; K Höld; M Vegter-van der Vlis; L A Sandkuijl; E Bakker; G J van Ommen
Journal:  Am J Hum Genet       Date:  1992-10       Impact factor: 11.025

7.  Genetic linkage between Huntington disease and the D4S10 locus in South African families: further evidence against non-allelic heterogeneity.

Authors:  L J Greenberg; R W Martell; J Theilman; M R Hayden; J Joubert
Journal:  Hum Genet       Date:  1991-10       Impact factor: 4.132
  7 in total

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