Literature DB >> 25631809

Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.

Daesub Song1, Gunwoo Ha2, Wissam Serhan3, Yassir Eltahir4, Mohammed Yusof3, Farouq Hashem3, Elsaeid Elsayed5, Bahaaeldin Marzoug5, Assem Abdelazim5, Salama Al Muhairi6.   

Abstract

We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 25631809      PMCID： PMC4365254          DOI： 10.1128/JCM.03096-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, June 2013.

Authors:  R A Perera; P Wang; M R Gomaa; R El-Shesheny; A Kandeil; O Bagato; L Y Siu; M M Shehata; A S Kayed; Y Moatasim; M Li; L L Poon; Y Guan; R J Webby; M A Ali; J S Peiris; G Kayali
Journal:  Euro Surveill       Date:  2013-09-05

2.  Contact investigation of a case of human novel coronavirus infection treated in a German hospital, October-November 2012.

Authors:  U Buchholz; M A Müller; A Nitsche; A Sanewski; N Wevering; T Bauer-Balci; F Bonin; C Drosten; B Schweiger; T Wolff; D Muth; B Meyer; S Buda; G Krause; L Schaade; W Haas
Journal:  Euro Surveill       Date:  2013-02-21

3.  Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia.

Authors:  Ali M Zaki; Sander van Boheemen; Theo M Bestebroer; Albert D M E Osterhaus; Ron A M Fouchier
Journal:  N Engl J Med       Date:  2012-10-17       Impact factor: 91.245

4.  Specific serology for emerging human coronaviruses by protein microarray.

Authors:  C Reusken; H Mou; G J Godeke; L van der Hoek; B Meyer; M A Müller; B Haagmans; R de Sousa; N Schuurman; U Dittmer; P Rottier; A Osterhaus; C Drosten; B J Bosch; M Koopmans
Journal:  Euro Surveill       Date:  2013-04-04

5.  Development and validation of an immunogold chromatographic test for on-farm detection of PRRSV.

Authors:  Sheng-hua Zhou; Shang-jin Cui; Chang-mu Chen; Fan-chao Zhang; Jun Li; Shun Zhou; Jin-Sik Oh
Journal:  J Virol Methods       Date:  2009-05-07       Impact factor: 2.014

6.  Evidence for camel-to-human transmission of MERS coronavirus.

Authors:  Esam I Azhar; Sherif A El-Kafrawy; Suha A Farraj; Ahmed M Hassan; Muneera S Al-Saeed; Anwar M Hashem; Tariq A Madani
Journal:  N Engl J Med       Date:  2014-06-04       Impact factor: 91.245

7.  Human betacoronavirus 2c EMC/2012-related viruses in bats, Ghana and Europe.

Authors:  Augustina Annan; Heather J Baldwin; Victor Max Corman; Stefan M Klose; Michael Owusu; Evans Ewald Nkrumah; Ebenezer Kofi Badu; Priscilla Anti; Olivia Agbenyega; Benjamin Meyer; Samuel Oppong; Yaw Adu Sarkodie; Elisabeth K V Kalko; Peter H C Lina; Elena V Godlevska; Chantal Reusken; Antje Seebens; Florian Gloza-Rausch; Peter Vallo; Marco Tschapka; Christian Drosten; Jan Felix Drexler
Journal:  Emerg Infect Dis       Date:  2013-03       Impact factor: 6.883

Review 8.  Middle East respiratory syndrome coronavirus antibody reactors among camels in Dubai, United Arab Emirates, in 2005.

Authors:  S Alexandersen; G P Kobinger; G Soule; U Wernery
Journal:  Transbound Emerg Dis       Date:  2014-01-24       Impact factor: 5.005

9.  Antibodies against MERS coronavirus in dromedary camels, United Arab Emirates, 2003 and 2013.

Authors:  Benjamin Meyer; Marcel A Müller; Victor M Corman; Chantal B E M Reusken; Daniel Ritz; Gert-Jan Godeke; Erik Lattwein; Stephan Kallies; Artem Siemens; Janko van Beek; Jan F Drexler; Doreen Muth; Berend-Jan Bosch; Ulrich Wernery; Marion P G Koopmans; Renate Wernery; Christian Drosten
Journal:  Emerg Infect Dis       Date:  2014-04       Impact factor: 6.883

10.  Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Authors:  Kazuya Shirato; Takuya Yano; Syouhei Senba; Shigehiro Akachi; Takashi Kobayashi; Takamichi Nishinaka; Tsugunori Notomi; Shutoku Matsuyama
Journal:  Virol J       Date:  2014-08-08       Impact factor: 4.099
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  21 in total

Review 1.  Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease.

Authors:  Jasper F W Chan; Susanna K P Lau; Kelvin K W To; Vincent C C Cheng; Patrick C Y Woo; Kwok-Yung Yuen
Journal:  Clin Microbiol Rev       Date:  2015-04       Impact factor: 26.132

Review 2.  MERS coronavirus: diagnostics, epidemiology and transmission.

Authors:  Ian M Mackay; Katherine E Arden
Journal:  Virol J       Date:  2015-12-22       Impact factor: 4.099

Review 3.  Development of Middle East Respiratory Syndrome Coronavirus vaccines - advances and challenges.

Authors:  Heeyoun Cho; Jean-Louis Excler; Jerome H Kim; In-Kyu Yoon
Journal:  Hum Vaccin Immunother       Date:  2017-11-29       Impact factor: 3.452

4.  Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.

Authors:  Jasper Fuk-Woo Chan; Garnet Kwan-Yue Choi; Alan Ka-Lun Tsang; Kah-Meng Tee; Ho-Yin Lam; Cyril Chik-Yan Yip; Kelvin Kai-Wang To; Vincent Chi-Chung Cheng; Man-Lung Yeung; Susanna Kar-Pui Lau; Patrick Chiu-Yat Woo; Kwok-Hung Chan; Bone Siu-Fai Tang; Kwok-Yung Yuen
Journal:  J Clin Microbiol       Date:  2015-05-27       Impact factor: 5.948

5.  Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests.

Authors:  Yutaro Yamaoka; Kei Miyakawa; Sundararaj Stanleyraj Jeremiah; Rikako Funabashi; Koji Okudela; Sayaka Kikuchi; Junichi Katada; Atsuhiko Wada; Toshiki Takei; Mayuko Nishi; Kohei Shimizu; Hiroki Ozawa; Shuzo Usuku; Chiharu Kawakami; Nobuko Tanaka; Takeshi Morita; Hiroyuki Hayashi; Hideaki Mitsui; Keita Suzuki; Daisuke Aizawa; Yukihiro Yoshimura; Tomoyuki Miyazaki; Etsuko Yamazaki; Tadaki Suzuki; Hirokazu Kimura; Hideaki Shimizu; Nobuhiko Okabe; Hideki Hasegawa; Akihide Ryo
Journal:  Cell Rep Med       Date:  2021-05-16

6.  Development of an immunochromatographic kit to detect severe acute respiratory syndrome coronavirus 2.

Authors:  Satoshi Oshiro; Yoko Tabe; Keiji Funatogawa; Kaori Saito; Tatsuya Tada; Tomomi Hishinuma; Naeko Mizutani; Makoto Akiwa; Jun-Ichiro Sekiguchi; Takashi Miida; Teruo Kirikae
Journal:  J Virol Methods       Date:  2021-05-10       Impact factor: 2.014

7.  Development of Monoclonal Antibody and Diagnostic Test for Middle East Respiratory Syndrome Coronavirus Using Cell-Free Synthesized Nucleocapsid Antigen.

Authors:  Yutaro Yamaoka; Shutoku Matsuyama; Shuetsu Fukushi; Satoko Matsunaga; Yuki Matsushima; Hiroyuki Kuroyama; Hirokazu Kimura; Makoto Takeda; Tomoyuki Chimuro; Akihide Ryo
Journal:  Front Microbiol       Date:  2016-04-20       Impact factor: 5.640

8.  Use of Piezoelectric Immunosensors for Detection of Interferon-Gamma Interaction with Specific Antibodies in the Presence of Released-Active Forms of Antibodies to Interferon-Gamma.

Authors:  Elena Don; Olga Farafonova; Suzanna Pokhil; Darya Barykina; Marina Nikiforova; Darya Shulga; Alena Borshcheva; Sergey Tarasov; Tatyana Ermolaeva; Oleg Epstein
Journal:  Sensors (Basel)       Date:  2016-01-20       Impact factor: 3.576

9.  Asymptomatic MERS-CoV Infection in Humans Possibly Linked to Infected Dromedaries Imported from Oman to United Arab Emirates, May 2015.

Authors:  Zulaikha M Al Hammadi; Daniel K W Chu; Yassir M Eltahir; Farida Al Hosani; Mariam Al Mulla; Wasim Tarnini; Aron J Hall; Ranawaka A P M Perera; Mohamed M Abdelkhalek; J S M Peiris; Salama S Al Muhairi; Leo L M Poon
Journal:  Emerg Infect Dis       Date:  2015-12       Impact factor: 6.883

10.  Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus.

Authors:  Kwang-Soo Lyoo; Minjoo Yeom; Jungho Kim; Donghyuk Kim; Gunwoo Ha; Woonsung Na; Van Phan Le; Daesub Song
Journal:  Vet Rec       Date:  2017-10-09       Impact factor: 2.695
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