Literature DB >> 23594517

Specific serology for emerging human coronaviruses by protein microarray.

C Reusken1, H Mou, G J Godeke, L van der Hoek, B Meyer, M A Müller, B Haagmans, R de Sousa, N Schuurman, U Dittmer, P Rottier, A Osterhaus, C Drosten, B J Bosch, M Koopmans.   

Abstract

We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

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Year:  2013        PMID: 23594517     DOI: 10.2807/1560-7917.es2013.18.14.20441

Source DB:  PubMed          Journal:  Euro Surveill        ISSN: 1025-496X


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