Literature DB >> 26019210

Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.

Jasper Fuk-Woo Chan1, Garnet Kwan-Yue Choi2, Alan Ka-Lun Tsang2, Kah-Meng Tee2, Ho-Yin Lam3, Cyril Chik-Yan Yip2, Kelvin Kai-Wang To1, Vincent Chi-Chung Cheng2, Man-Lung Yeung2, Susanna Kar-Pui Lau1, Patrick Chiu-Yat Woo1, Kwok-Hung Chan2, Bone Siu-Fai Tang3, Kwok-Yung Yuen4.   

Abstract

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 26019210      PMCID： PMC4508434          DOI： 10.1128/JCM.01224-15

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  41 in total

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2.  Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia.

Authors:  Patrick C Y Woo; Susanna K P Lau; Chung-ming Chu; Kwok-hung Chan; Hoi-wah Tsoi; Yi Huang; Beatrice H L Wong; Rosana W S Poon; James J Cai; Wei-kwang Luk; Leo L M Poon; Samson S Y Wong; Yi Guan; J S Malik Peiris; Kwok-yung Yuen
Journal:  J Virol       Date:  2005-01       Impact factor: 5.103

3.  Development and validation of a rapid immunochromatographic assay for detection of Middle East respiratory syndrome coronavirus antigen in dromedary camels.

Authors:  Daesub Song; Gunwoo Ha; Wissam Serhan; Yassir Eltahir; Mohammed Yusof; Farouq Hashem; Elsaeid Elsayed; Bahaaeldin Marzoug; Assem Abdelazim; Salama Al Muhairi
Journal:  J Clin Microbiol       Date:  2015-01-28       Impact factor: 5.948

4.  Molecular analysis of the human coronavirus (strain 229E) genome.

Authors:  J Herold; T Raabe; S Siddell
Journal:  Arch Virol Suppl       Date:  1993

5.  Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome.

Authors:  Raymond K H Hui; Fanya Zeng; Charis M N Chan; K Y Yuen; Joseph S M Peiris; Frederick C C Leung
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

6.  SARS coronavirus detection methods.

Authors:  Susanna K P Lau; Xiao-Yan Che; Patrick C Y Woo; Beatrice H L Wong; Vincent C C Cheng; Gibson K S Woo; Ivan F N Hung; Rosana W S Poon; Kwok-Hung Chan; J S Malik Peiris; Kwok-Yung Yuen
Journal:  Emerg Infect Dis       Date:  2005-07       Impact factor: 6.883

Review 7.  The structure and functions of coronavirus genomic 3' and 5' ends.

Authors:  Dong Yang; Julian L Leibowitz
Journal:  Virus Res       Date:  2015-02-28       Impact factor: 3.303

8.  Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43.

Authors:  Xiao-Yan Che; Li-Wen Qiu; Zhi-Yong Liao; Ya-di Wang; Kun Wen; Yu-Xian Pan; Wei Hao; Ya-Bo Mei; Vincent C C Cheng; Kwok-Yung Yuen
Journal:  J Infect Dis       Date:  2005-05-06       Impact factor: 5.226

Review 9.  A systematic approach to novel virus discovery in emerging infectious disease outbreaks.

Authors:  Siddharth Sridhar; Kelvin K W To; Jasper F W Chan; Susanna K P Lau; Patrick C Y Woo; Kwok-Yung Yuen
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10.  Genome structure and transcriptional regulation of human coronavirus NL63.

Authors:  Krzysztof Pyrc; Maarten F Jebbink; Ben Berkhout; Lia van der Hoek
Journal:  Virol J       Date:  2004-11-17       Impact factor: 4.099
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  44 in total

Review 1.  MERS coronavirus: diagnostics, epidemiology and transmission.

Authors:  Ian M Mackay; Katherine E Arden
Journal:  Virol J       Date:  2015-12-22       Impact factor: 4.099

Review 2.  Development of Middle East Respiratory Syndrome Coronavirus vaccines - advances and challenges.

Authors:  Heeyoun Cho; Jean-Louis Excler; Jerome H Kim; In-Kyu Yoon
Journal:  Hum Vaccin Immunother       Date:  2017-11-29       Impact factor: 3.452

Review 3.  Emerging role of nanomedicine in the treatment of neuropathic pain.

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4.  Current Challenges for the Effective Management of the COVID-19 Pandemic.

Authors:  Vibha Sinha; Saurabh Saxena; Sanjay Kumar Pandey; Sudhakar Dwivedi; Suresh Thakur; Alexzander Asea; Ashwini Kumar Dixit; Vineeta Dixit; Naveen Kumar Vishvakarma; Dhananjay Shukla
Journal:  Adv Exp Med Biol       Date:  2021       Impact factor: 2.622

Review 5.  Mass screening is a key component to fight against SARS-CoV-2 and return to normalcy.

Authors:  Zhaomin Feng; Yi Zhang; Yang Pan; Daitao Zhang; Lei Zhang; Quanyi Wang
Journal:  Med Rev (Berl)       Date:  2022-04-28

Review 6.  Middle East respiratory syndrome coronavirus infection: virus-host cell interactions and implications on pathogenesis.

Authors:  Jie Zhou; Hin Chu; Jasper Fuk-Woo Chan; Kwok-Yung Yuen
Journal:  Virol J       Date:  2015-12-22       Impact factor: 4.099

7.  Differential cell line susceptibility to the emerging Zika virus: implications for disease pathogenesis, non-vector-borne human transmission and animal reservoirs.

Authors:  Jasper Fuk-Woo Chan; Cyril Chik-Yan Yip; Jessica Oi-Ling Tsang; Kah-Meng Tee; Jian-Piao Cai; Kenn Ka-Heng Chik; Zheng Zhu; Chris Chung-Sing Chan; Garnet Kwan-Yue Choi; Siddharth Sridhar; Anna Jinxia Zhang; Gang Lu; Kin Chiu; Amy Cheuk-Yin Lo; Sai-Wah Tsao; Kin-Hang Kok; Dong-Yan Jin; Kwok-Hung Chan; Kwok-Yung Yuen
Journal:  Emerg Microbes Infect       Date:  2016-08-24       Impact factor: 7.163

8.  Zika Virus Infection in Dexamethasone-immunosuppressed Mice Demonstrating Disseminated Infection with Multi-organ Involvement Including Orchitis Effectively Treated by Recombinant Type I Interferons.

Authors:  Jasper Fuk-Woo Chan; Anna Jinxia Zhang; Chris Chung-Sing Chan; Cyril Chik-Yan Yip; Winger Wing-Nga Mak; Houshun Zhu; Vincent Kwok-Man Poon; Kah-Meng Tee; Zheng Zhu; Jian-Piao Cai; Jessica Oi-Ling Tsang; Kenn Ka-Heng Chik; Feifei Yin; Kwok-Hung Chan; Kin-Hang Kok; Dong-Yan Jin; Rex Kwok-Him Au-Yeung; Kwok-Yung Yuen
Journal:  EBioMedicine       Date:  2016-11-12       Impact factor: 8.143

9.  Performance Evaluation of the PowerChek MERS (upE & ORF1a) Real-Time PCR Kit for the Detection of Middle East Respiratory Syndrome Coronavirus RNA.

Authors:  Hee Jae Huh; Ji Youn Kim; Hyeon Jeong Kwon; Sun Ae Yun; Myoung Keun Lee; Chang Seok Ki; Nam Yong Lee; Jong Won Kim
Journal:  Ann Lab Med       Date:  2017-11       Impact factor: 3.464

10.  Clofazimine broadly inhibits coronaviruses including SARS-CoV-2.

Authors:  Shuofeng Yuan; Xin Yin; Xiangzhi Meng; Jasper Fuk-Woo Chan; Zi-Wei Ye; Laura Riva; Lars Pache; Chris Chun-Yiu Chan; Pok-Man Lai; Chris Chung-Sing Chan; Vincent Kwok-Man Poon; Andrew Chak-Yiu Lee; Naoko Matsunaga; Yuan Pu; Chun-Kit Yuen; Jianli Cao; Ronghui Liang; Kaiming Tang; Li Sheng; Yushen Du; Wan Xu; Chit-Ying Lau; Ko-Yung Sit; Wing-Kuk Au; Runming Wang; Yu-Yuan Zhang; Yan-Dong Tang; Thomas Mandel Clausen; Jessica Pihl; Juntaek Oh; Kong-Hung Sze; Anna Jinxia Zhang; Hin Chu; Kin-Hang Kok; Dong Wang; Xue-Hui Cai; Jeffrey D Esko; Ivan Fan-Ngai Hung; Ronald Adolphus Li; Honglin Chen; Hongzhe Sun; Dong-Yan Jin; Ren Sun; Sumit K Chanda; Kwok-Yung Yuen
Journal:  Nature       Date:  2021-03-16       Impact factor: 49.962
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