| Literature DB >> 25618336 |
Matthew R Broadus1, P Renee Yew, Stephen R Hann, Ethan Lee.
Abstract
Screens for small-molecule modulators of biological pathways typically utilize cultured cell lines, purified proteins, or, recently, model organisms (e.g., zebrafish, Drosophila, C. elegans). Herein, we describe a method for using Xenopus laevis egg extract, a biologically active and highly tractable cell-free system that recapitulates a legion of complex chemical reactions found in intact cells. Specifically, we focus on the use of a luciferase-based fusion system to identify small-molecule modulators that affect protein turnover.Entities:
Mesh：
Substances：
Year: 2015 PMID： 25618336 PMCID： PMC4492114 DOI： 10.1007/978-1-4939-2269-7_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745