José Alberto Conde-Aguilera1,2, Louis Lefaucheur1,2, Sophie Tesseraud3, Yves Mercier4, Nathalie Le Floc'h1,2, Jaap van Milgen5,6. 1. INRA, UMR1348 PEGASE, 35590, Saint-Gilles, France. 2. Agrocampus Ouest, UMR1348 PEGASE, 35000, Rennes, France. 3. INRA, UR83 Recherches Avicoles, 37380, Nouzilly, France. 4. Adisseo France SAS, 92160, Antony, France. 5. INRA, UMR1348 PEGASE, 35590, Saint-Gilles, France. jaap.vanmilgen@rennes.inra.fr. 6. Agrocampus Ouest, UMR1348 PEGASE, 35000, Rennes, France. jaap.vanmilgen@rennes.inra.fr.
Abstract
PURPOSE: Although amino acids (AA) are required for growth, little is known about the effect of a deficient AA supply on the composition and the contractile and metabolic properties of skeletal muscles. METHODS: Protein metabolism, oxidative catabolism, glutathione system, and fiber-type composition of the longissimus (LM), rhomboideus (RM), and semitendinous (SM) muscles were compared between 42-day-old piglets pair-fed for 10 days either with a diet with a 28% deficient supply of total sulfur AA (TSAA-) or with a diet with a sufficient supply of total sulfur AA (TSAA+). RESULTS: The relative weight, protein mass, and protein synthesis of LM were 10-32% lower in TSAA- pigs compared with TSAA+ pigs, while RM and SM were not affected by the TSAA supply. The TSAA supply affected the AA composition of muscles. Concentrations of Met and branched-chain AA were, respectively, 7 and 3% lower in TSAA- pigs compared with TSAA+ pigs. The His concentration was 30% higher in LM and SM in TSAA- pigs compared with TSAA+ pigs and unaffected in RM. The activity of citrate synthase was 14% higher in all three muscles of TSAA- pigs. In these pigs, the β-hydroxy-acyl-CoA dehydrogenase activity was 20% higher in RM compared with TSAA+ pigs while that of lactate dehydrogenase was 21% lower in LM. Total and reduced glutathione concentrations were more than 70% greater in RM than in LM or SM, and these concentrations were approximately 10% lower in TSAA- pigs than in TSAA+ pigs. CONCLUSIONS: Results of this study indicate that a TSAA deficiency affects muscle properties in a muscle-dependent manner increasing the oxidative capacity of RM and reducing growth and glycolytic metabolism of LM.
PURPOSE: Although amino acids (AA) are required for growth, little is known about the effect of a deficient AA supply on the composition and the contractile and metabolic properties of skeletal muscles. METHODS: Protein metabolism, oxidative catabolism, glutathione system, and fiber-type composition of the longissimus (LM), rhomboideus (RM), and semitendinous (SM) muscles were compared between 42-day-old piglets pair-fed for 10 days either with a diet with a 28% deficient supply of total sulfur AA (TSAA-) or with a diet with a sufficient supply of total sulfur AA (TSAA+). RESULTS: The relative weight, protein mass, and protein synthesis of LM were 10-32% lower in TSAA- pigs compared with TSAA+ pigs, while RM and SM were not affected by the TSAA supply. The TSAA supply affected the AA composition of muscles. Concentrations of Met and branched-chain AA were, respectively, 7 and 3% lower in TSAA- pigs compared with TSAA+ pigs. The His concentration was 30% higher in LM and SM in TSAA- pigs compared with TSAA+ pigs and unaffected in RM. The activity of citrate synthase was 14% higher in all three muscles of TSAA- pigs. In these pigs, the β-hydroxy-acyl-CoA dehydrogenase activity was 20% higher in RM compared with TSAA+ pigs while that of lactate dehydrogenase was 21% lower in LM. Total and reduced glutathione concentrations were more than 70% greater in RM than in LM or SM, and these concentrations were approximately 10% lower in TSAA- pigs than in TSAA+ pigs. CONCLUSIONS: Results of this study indicate that a TSAA deficiency affects muscle properties in a muscle-dependent manner increasing the oxidative capacity of RM and reducing growth and glycolytic metabolism of LM.
Authors: José Alberto Conde-Aguilera; Nathalie Le Floc'h; Isabelle Le Huërou-Luron; Yves Mercier; Sophie Tesseraud; Louis Lefaucheur; Jaap van Milgen Journal: Eur J Nutr Date: 2015-09-03 Impact factor: 5.614
Authors: Rosa Castellano; Marie-Hélène Perruchot; José Alberto Conde-Aguilera; Jaap van Milgen; Anne Collin; Sophie Tesseraud; Yves Mercier; Florence Gondret Journal: PLoS One Date: 2015-07-10 Impact factor: 3.240