| Literature DB >> 25560074 |
Bo Zhao1, Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Han Zhou, Mengnan Zhang, Jun Yin.
Abstract
Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. PPTases have been widely used for site-specific protein labeling and live cell imaging of the target proteins. Here we describe the use of PPTase-catalyzed protein labeling in protein engineering by facilitating high-throughput phage selection.Entities:
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Year: 2015 PMID： 25560074 PMCID： PMC4648548 DOI： 10.1007/978-1-4939-2272-7_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745