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Literature DB >> 2554308

Cell proteins bind to multiple sites within the 5' untranslated region of poliovirus RNA.

R M del Angel¹, A G Papavassiliou, C Fernández-Tomás, S J Silverstein, V R Racaniello.

Abstract

The 5' noncoding region of poliovirus RNA contains sequences necessary for translation and replication. These functions are probably carried out by recognition of poliovirus RNA by cellular and/or viral proteins. Using a mobility-shift electrophoresis assay and 1,10-phenanthroline/Cu+ footprinting, we demonstrate specific binding of cytoplasmic factors with a sequence from nucleotides 510-629 within the 5' untranslated region (UTR). Complex formation was also observed with a second sequence (nucleotides 97-182) within the 5' UTR. These two regions of the 5' UTR appear to be recognized by distinct cell factors as determined by competition analysis and the effects of ionic strength on complex formation. However, both complexes contain eukaryotic initiation factor 2 alpha, as revealed by their reaction with specific antibody.

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Year: 1989 PMID： 2554308 PMCID： PMC298268 DOI： 10.1073/pnas.86.21.8299

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

22 in total

1. New model for the secondary structure of the 5' non-coding RNA of poliovirus is supported by biochemical and genetic data that also show that RNA secondary structure is important in neurovirulence.

Authors: M A Skinner; V R Racaniello; G Dunn; J Cooper; P D Minor; J W Almond
Journal: J Mol Biol Date: 1989-05-20 Impact factor: 5.469

2. Construction of viable deletion and insertion mutants of the Sabin strain of type 1 poliovirus: function of the 5' noncoding sequence in viral replication.

Authors: S Kuge; A Nomoto
Journal: J Virol Date: 1987-05 Impact factor: 5.103

3. An RNA sequence of hundreds of nucleotides at the 5' end of poliovirus RNA is involved in allowing viral protein synthesis.

Authors: D Trono; R Andino; D Baltimore
Journal: J Virol Date: 1988-07 Impact factor: 5.103

4. Comparative sequence analysis of the 5' noncoding region of the enteroviruses and rhinoviruses.

Authors: V M Rivera; J D Welsh; J V Maizel
Journal: Virology Date: 1988-07 Impact factor: 3.616

5. An internal 5'-noncoding region required for translation of poliovirus RNA in vitro.

Authors: K Bienkowska-Szewczyk; E Ehrenfeld
Journal: J Virol Date: 1988-08 Impact factor: 5.103

6. Footprinting DNA-protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coli RNA polymerase-lac promoter complexes.

Authors: M D Kuwabara; D S Sigman
Journal: Biochemistry Date: 1987-11-17 Impact factor: 3.162

7. Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing.

Authors: N La Monica; C Meriam; V R Racaniello
Journal: J Virol Date: 1986-02 Impact factor: 5.103

8. Phosphorylation of eukaryotic initiation factor 2 during physiological stresses which affect protein synthesis.

Authors: K A Scorsone; R Panniers; A G Rowlands; E C Henshaw
Journal: J Biol Chem Date: 1987-10-25 Impact factor: 5.157

9. Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.

Authors: J Pelletier; G Kaplan; V R Racaniello; N Sonenberg
Journal: Mol Cell Biol Date: 1988-03 Impact factor: 4.272

10. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA.

Authors: J Pelletier; N Sonenberg
Journal: Nature Date: 1988-07-28 Impact factor: 49.962

66 in total

1. Cap-independent translation by the 5' untranslated region of Theiler's murine encephalomyelitis virus.

Authors: P K Bandyopadhyay; C Wang; H L Lipton
Journal: J Virol Date: 1992-11 Impact factor: 5.103

2. Specific interactions of HeLa cell proteins with proposed translation domains of the poliovirus 5' noncoding region.

Authors: J R Gebhard; E Ehrenfeld
Journal: J Virol Date: 1992-05 Impact factor: 5.103

3. Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5' noncoding region of the genome involved in internal initiation of protein synthesis.

Authors: N Percy; G J Belsham; J K Brangwyn; M Sullivan; D M Stone; J W Almond
Journal: J Virol Date: 1992-03 Impact factor: 5.103

10. An infectious cDNA copy of the genome of a non-cardiovirulent coxsackievirus B3 strain: its complete sequence analysis and comparison to the genomes of cardiovirulent coxsackieviruses.

Authors: N M Chapman; Z Tu; S Tracy; C J Gauntt
Journal: Arch Virol Date: 1994 Impact factor: 2.574