Zhiying Yan1, Zilin Song1, Dong Li1, Yuexiang Yuan1, Xiaofeng Liu2, Tao Zheng3. 1. Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China. 2. Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China. Electronic address: lxf3636@163.com. 3. Nanjing Normal University, Nanjing 210023, China.
Abstract
This study investigated the possibilities of improving the biogasification from solid-state anaerobic digestion (SS-AD) of composting rice straw (RS) based on the optimized digestion temperature, initial substrate concentration (ISC) and C/N ratio. RS compounds, such as lignin, cellulose, and hemicellulose, were significantly degraded after composting. A significant interactive effect of temperature, ISC and C/N ratio was found on the biogasification of SS-AD of composting RS, and a maximum biogas production was achieved at 35.6°C, with a 20% ISC and a C/N ratio of 29.6:1. The verification experiment confirmed the optimization results. High-throughput sequencing analysis indicated that microbial communities in the SS-AD mainly consist of Methanobacteria, Bacteroidia, Clostridia, Betaproteobacteria, and Gammaproteobacteria. A dominant Methanobacteria was shifted from Methanobacterium to Methanoculleus during the SS-AD process. This study provides novel information about the interdependent effects and microbial behavior of AD.
This study investigated the possibilities of improving the biogasification from solid-state anaerobic digestion (SS-AD) of composting rice straw (n class="Chemical">RS) based on the optimized digestion temperature, initial substrate concentration (ISC) and C/N ratio. RS compounds, such as lignin, cellulose, and hemicellulose, were significantly degraded after composting. A significant interactive effect of temperature, ISC and C/N ratio was found on the biogasification of SS-AD of composting RS, and a maximum biogas production was achieved at 35.6°C, with a 20% ISC and a C/N ratio of 29.6:1. The verification experiment confirmed the optimization results. High-throughput sequencing analysis indicated that microbial communities in the SS-AD mainly consist of Methanobacteria, Bacteroidia, Clostridia, Betaproteobacteria, and Gammaproteobacteria. A dominant Methanobacteria was shifted from Methanobacterium to Methanoculleus during the SS-AD process. This study provides novel information about the interdependent effects and microbial behavior of AD.