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Literature DB >> 25482641

Transgene expression study of CXCR4 active mutants. Potential prospects in up-modulation of homing and engraftment efficiency of hematopoietic stem/progenitor cells.

Menka Sharma¹, Farhat Afrin, Rajendra P Tripathi, Gurudutta Gangenahalli.

Abstract

Homing and engraftment, a determining factor in hematopoietic stem cell transplantation success is defined as a process through which hematopoietic stem/progenitor cells (HSPCs) lodge recipient bone marrow. SDF-1/CXCR4 axis acts as a principle regulator in homing and engraftment, however, CXCR4 signaling is dependent upon expression of CXCR4 and its ligand SDF-1, which is highly dynamic. Hence, present investigation was aimed to explore the potential of CXCR4 constitutive active mutants (CXCR4-CAMs) in overcoming the limitation of CXCR4 signaling and up-modulate its efficiency in homing and engraftment. Regulated transgene expression study of these mutants revealed their significantly enhanced cell adhesion efficiency to endothelium and extracellular matrix protein. This altogether indicates promising prospects of CXCR4-CAMs in research aimed to improve HSPCs engraftment efficiency.

Entities: Gene

Keywords: Ala, Alanine; Asn, Asparagine; BM, Bone Marrow; BMEC, Bone marrow endothelial cells; BSA, Bovine Serum Albumin; CAMs, Constitutive Active Mutants; CXCR4; Conc., Concentration; ECM, Extracellular matrix; FBS, Fetal Bovine Serum; FN, Fibronectin; HSPCs; HSPCs, Hematopoietic Stem/ Progenitor Cells; HUVECs, Human Umbilical Vein Endothelial cells; IMDM, Iscove's Modified Dulbecco Media; LIF, Leukemia Inhibitory Factor; MCS, Multi Cloning Site; Ser, Serine; TM3, Transmembrane three domain; engraftment; homing

Mesh：

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Year: 2014 PMID： 25482641 PMCID： PMC4594461 DOI： 10.4161/cam.29285

Source DB: PubMed Journal: Cell Adh Migr ISSN： 1933-6918 Impact factor: 3.405

22 in total

Review 1. Regulation of CXCR4 signaling.

Authors: John M Busillo; Jeffrey L Benovic
Journal: Biochim Biophys Acta Date: 2006-11-10

2. The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34(+) cells: role in transendothelial/stromal migration and engraftment of NOD/SCID mice.

Authors: A Peled; O Kollet; T Ponomaryov; I Petit; S Franitza; V Grabovsky; M M Slav; A Nagler; O Lider; R Alon; D Zipori; T Lapidot
Journal: Blood Date: 2000-06-01 Impact factor: 22.113

3. Impaired B-lymphopoiesis, myelopoiesis, and derailed cerebellar neuron migration in CXCR4- and SDF-1-deficient mice.

Authors: Q Ma; D Jones; P R Borghesani; R A Segal; T Nagasawa; T Kishimoto; R T Bronson; T A Springer
Journal: Proc Natl Acad Sci U S A Date: 1998-08-04 Impact factor: 11.205

4. The alpha-chemokine, stromal cell-derived factor-1alpha, binds to the transmembrane G-protein-coupled CXCR-4 receptor and activates multiple signal transduction pathways.

Authors: R K Ganju; S A Brubaker; J Meyer; P Dutt; Y Yang; S Qin; W Newman; J E Groopman
Journal: J Biol Chem Date: 1998-09-04 Impact factor: 5.157

Review 5. Properties and usefulness of the original K-562 human myelogenous leukemia cell line.

Authors: B B Lozzio; C B Lozzio
Journal: Leuk Res Date: 1979 Impact factor: 3.156

6. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4.

Authors: A Peled; I Petit; O Kollet; M Magid; T Ponomaryov; T Byk; A Nagler; H Ben-Hur; A Many; L Shultz; O Lider; R Alon; D Zipori; T Lapidot
Journal: Science Date: 1999-02-05 Impact factor: 47.728

Review 7. Inducible endothelial functions in inflammation and coagulation.

Authors: M P Bevilacqua; M A Gimbrone
Journal: Semin Thromb Hemost Date: 1987-10 Impact factor: 4.180

Review 8. Inducible gene expression systems for higher eukaryotic cells.

Authors: M Gossen; A L Bonin; S Freundlieb; H Bujard
Journal: Curr Opin Biotechnol Date: 1994-10 Impact factor: 9.740

9. Up-regulation of CXCR4 expression in PC-3 cells by stromal-derived factor-1alpha (CXCL12) increases endothelial adhesion and transendothelial migration: role of MEK/ERK signaling pathway-dependent NF-kappaB activation.

Authors: Promil Kukreja; Asim B Abdel-Mageed; Debasis Mondal; Kai Liu; Krishna C Agrawal
Journal: Cancer Res Date: 2005-11-01 Impact factor: 12.701

10. Overexpression of CXCR4 on human CD34+ progenitors increases their proliferation, migration, and NOD/SCID repopulation.

Authors: Joy Kahn; Tamara Byk; Lottie Jansson-Sjostrand; Isabelle Petit; Shoham Shivtiel; Arnon Nagler; Izhar Hardan; Varda Deutsch; Zulma Gazit; Dan Gazit; Stefan Karlsson; Tsvee Lapidot
Journal: Blood Date: 2003-12-24 Impact factor: 22.113

4 in total

1. Stromal cell-derived factor-1-directed bone marrow mesenchymal stem cell migration in response to inflammatory and/or hypoxic stimuli.

Authors: Yang Yu; Rui-Xin Wu; Li-Na Gao; Yu Xia; Hao-Ning Tang; Fa-Ming Chen
Journal: Cell Adh Migr Date: 2016-01-08 Impact factor: 3.405