Zeineb Jemaa1, Amani Kallel1, Chaker Sleimi1, Imen Mahjoubi1, Moncef Feki1, Bochra Ftouhi2, Hedia Slimane2, Riadh Jemaa3, Naziha Kaabachi1. 1. LR99ES11 Research Laboratory, Department of Biochemistry, Rabta Hospital, University of Tunis El Manar, Tunis, Tunisia. 2. Department of Endocrinology, Rabta Hospital, Tunis, Tunisia. 3. LR99ES11 Research Laboratory, Department of Biochemistry, Rabta Hospital, University of Tunis El Manar, Tunis, Tunisia. Electronic address: jemaa_riadh@yahoo.fr.
Abstract
BACKGROUND AND AIMS: Peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α) is a transcriptional co-activator involved in adaptive thermogenesis, skeletal muscle metabolism, fatty acid oxidation, and gluconeogenesis. Several studies have suggested that the common PGC-1α polymorphism Gly482Ser (rs8192678) may be associated with risk of type 2 diabetes (T2D), with conflicting results. The aim of this study was to analyze whether the Gly482Ser variant is a risk factor for development of T2D in Tunisian population. METHODS: In a case-control study 487 unrelated patients with type 2 diabetes and 402 apparently healthy controls were recruited from January 2008 to August 2010. The Gly482Ser polymorphism was determined by PCR-RFLP analysis. RESULTS: A significant difference in genotypes distribution was observed between patients (Gly/Gly: 34.1%; Gly/Ser: 47.1%; Ser/Ser: 18.5%) and controls (Gly/Gly: 43.8%; Gly/Ser: 42.3%; Ser/Ser: 13.9%) (χ(2)=9.44, p=0.009). The T2D patient group showed a significant higher frequency of the Ser allele compared to the controls (43% vs. 34%; OR: 1.35, 95% [CI]: 1.11-1.65, p=0.002). The association between the Gly482Ser polymorphism and T2D remained significant after adjustment for other well-established cardiovascular risk factors. CONCLUSIONS: In the current study, a significant and independent association between the Gly482Ser polymorphism (rs8192678) of the PGC-1α gene and T2D in the Tunisian population was found.
BACKGROUND AND AIMS: Peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α) is a transcriptional co-activator involved in adaptive thermogenesis, skeletal muscle metabolism, fatty acid oxidation, and gluconeogenesis. Several studies have suggested that the common PGC-1α polymorphism Gly482Ser (rs8192678) may be associated with risk of type 2 diabetes (T2D), with conflicting results. The aim of this study was to analyze whether the Gly482Ser variant is a risk factor for development of T2D in Tunisian population. METHODS: In a case-control study 487 unrelated patients with type 2 diabetes and 402 apparently healthy controls were recruited from January 2008 to August 2010. The Gly482Ser polymorphism was determined by PCR-RFLP analysis. RESULTS: A significant difference in genotypes distribution was observed between patients (Gly/Gly: 34.1%; Gly/Ser: 47.1%; Ser/Ser: 18.5%) and controls (Gly/Gly: 43.8%; Gly/Ser: 42.3%; Ser/Ser: 13.9%) (χ(2)=9.44, p=0.009). The T2D patient group showed a significant higher frequency of the Ser allele compared to the controls (43% vs. 34%; OR: 1.35, 95% [CI]: 1.11-1.65, p=0.002). The association between the Gly482Ser polymorphism and T2D remained significant after adjustment for other well-established cardiovascular risk factors. CONCLUSIONS: In the current study, a significant and independent association between the Gly482Ser polymorphism (rs8192678) of the PGC-1α gene and T2D in the Tunisian population was found.
Authors: Tessa Schillemans; Vinicius Tragante; Buamina Maitusong; Bruna Gigante; Sharon Cresci; Federica Laguzzi; Max Vikström; Mark Richards; Anna Pilbrow; Vicky Cameron; Luisa Foco; Robert N Doughty; Pekka Kuukasjärvi; Hooman Allayee; Jaana A Hartiala; W H Wilson Tang; Leo-Pekka Lyytikäinen; Kjell Nikus; Jari O Laurikka; Sundararajan Srinivasan; Ify R Mordi; Stella Trompet; Adriaan Kraaijeveld; Jessica van Setten; Crystel M Gijsberts; Anke H Maitland-van der Zee; Christoph H Saely; Yan Gong; Julie A Johnson; Rhonda M Cooper-DeHoff; Carl J Pepine; Gavino Casu; Andreas Leiherer; Heinz Drexel; Benjamin D Horne; Sander W van der Laan; Nicola Marziliano; Stanley L Hazen; Juha Sinisalo; Mika Kähönen; Terho Lehtimäki; Chim C Lang; Ralph Burkhardt; Markus Scholz; J Wouter Jukema; Niclas Eriksson; Axel Åkerblom; Stefan James; Claes Held; Emil Hagström; John A Spertus; Ale Algra; Ulf de Faire; Agneta Åkesson; Folkert W Asselbergs; Riyaz S Patel; Karin Leander Journal: Front Physiol Date: 2022-06-23 Impact factor: 4.755
Authors: José L Santos; Bernardo J Krause; Luis Rodrigo Cataldo; Javier Vega; Francisca Salas-Pérez; Paula Mennickent; Raúl Gallegos; Fermín I Milagro; Pedro Prieto-Hontoria; J Ignacio Riezu-Boj; Carolina Bravo; Albert Salas-Huetos; Ana Arpón; José E Galgani; J Alfredo Martínez Journal: Nutrients Date: 2020-09-11 Impact factor: 5.717