Literature DB >> 25465387

13Cα CEST experiment on uniformly 13C-labeled proteins.

Yang Zhou1, Daiwen Yang.   

Abstract

A new HSQC-based (13)Cα CEST pulse scheme is proposed, which is suitable for uniformly (13)C- or (13)C, (15)N-labeled samples in either water or heavy water. Except for Thr and Ser residues, the sensitivity of this scheme for uniformly labeled samples is similar to that of the previous scheme for selectively (13)Cα-labeled samples with 100% isotope enrichment. The experiment is demonstrated on an acyl carrier protein domain. Our (13)Cα CEST data reveal that the minor state of the acyl carrier protein has high helical propensity. The new scheme will facilitate structural characterization of invisible minor states.

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Year:  2014        PMID： 25465387     DOI： 10.1007/s10858-014-9888-1

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  19 in total

1.  A 2D ¹³C-CEST experiment for studying slowly exchanging protein systems using methyl probes: an application to protein folding.

Authors:  Guillaume Bouvignies; Lewis E Kay
Journal:  J Biomol NMR       Date:  2012-06-12       Impact factor: 2.835

Review 2.  New tools provide new insights in NMR studies of protein dynamics.

Authors:  Anthony Mittermaier; Lewis E Kay
Journal:  Science       Date:  2006-04-14       Impact factor: 47.728

3.  Studying "invisible" excited protein states in slow exchange with a major state conformation.

Authors:  Pramodh Vallurupalli; Guillaume Bouvignies; Lewis E Kay
Journal:  J Am Chem Soc       Date:  2012-05-03       Impact factor: 15.419

4.  Rigidifying acyl carrier protein domain in iterative type I PKS CalE8 does not affect its function.

Authors:  Jackwee Lim; Huihua Sun; Jing-Song Fan; Iman Fahim Hameed; Julien Lescar; Zhao-Xun Liang; Daiwen Yang
Journal:  Biophys J       Date:  2012-09-05       Impact factor: 4.033

5.  Probing slowly exchanging protein systems via ¹³Cα-CEST: monitoring folding of the Im7 protein.

Authors:  Alexandar L Hansen; Guillaume Bouvignies; Lewis E Kay
Journal:  J Biomol NMR       Date:  2013-02-06       Impact factor: 2.835

6.  Effects of J couplings and unobservable minor states on kinetics parameters extracted from CEST data.

Authors:  Yang Zhou; Daiwen Yang
Journal:  J Magn Reson       Date:  2014-10-31       Impact factor: 2.229

Review 7.  Characterization of enzyme motions by solution NMR relaxation dispersion.

Authors:  J Patrick Loria; Rebecca B Berlow; Eric D Watt
Journal:  Acc Chem Res       Date:  2008-02-19       Impact factor: 22.384

8.  Amino acid type determination in the sequential assignment procedure of uniformly 13C/15N-enriched proteins.

Authors:  S Grzesiek; A Bax
Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835

9.  A general method for assigning NMR spectra of denatured proteins using 3D HC(CO)NH-TOCSY triple resonance experiments.

Authors:  T M Logan; E T Olejniczak; R X Xu; S W Fesik
Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835

10.  Atomic-resolution dynamics on the surface of amyloid-β protofibrils probed by solution NMR.

Authors:  Nicolas L Fawzi; Jinfa Ying; Rodolfo Ghirlando; Dennis A Torchia; G Marius Clore
Journal:  Nature       Date:  2011-10-30       Impact factor: 49.962
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  5 in total

1.  A New Method for Determining Structure Ensemble: Application to a RNA Binding Di-Domain Protein.

Authors:  Wei Liu; Jingfeng Zhang; Jing-Song Fan; Giancarlo Tria; Gerhard Grüber; Daiwen Yang
Journal:  Biophys J       Date:  2016-05-10       Impact factor: 4.033

2.  Equilibrium folding dynamics of meACP in water, heavy water, and low concentration of urea.

Authors:  Yang Zhou; Daiwen Yang
Journal:  Sci Rep       Date:  2017-11-23       Impact factor: 4.379

Review 3.  Isotope Labels Combined with Solution NMR Spectroscopy Make Visible the Invisible Conformations of Small-to-Large RNAs.

Authors:  Theodore K Dayie; Lukasz T Olenginski; Kehinde M Taiwo
Journal:  Chem Rev       Date:  2022-04-20       Impact factor: 72.087

4.  Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations.

Authors:  Andrew P Longhini; Regan M LeBlanc; Owen Becette; Carolina Salguero; Christoph H Wunderlich; Bruce A Johnson; Victoria M D'Souza; Christoph Kreutz; T Kwaku Dayie
Journal:  Nucleic Acids Res       Date:  2015-12-10       Impact factor: 16.971

5.  Coexistence of multiple minor states of fatty acid binding protein and their functional relevance.

Authors:  Binhan Yu; Daiwen Yang
Journal:  Sci Rep       Date:  2016-09-28       Impact factor: 4.379
  5 in total

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