| Literature DB >> 25446899 |
Sonia A Melo1, Hikaru Sugimoto1, Joyce T O'Connell2, Noritoshi Kato2, Alberto Villanueva3, August Vidal4, Le Qiu5, Edward Vitkin5, Lev T Perelman5, Carlos A Melo6, Anthony Lucci7, Cristina Ivan8, George A Calin9, Raghu Kalluri10.
Abstract
Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into mature miRNAs. Pre-miRNAs, along with Dicer, AGO2, and TRBP, are present in exosomes of cancer cells. CD43 mediates the accumulation of Dicer specifically in cancer exosomes. Cancer exosomes mediate an efficient and rapid silencing of mRNAs to reprogram the target cell transcriptome. Exosomes derived from cells and sera of patients with breast cancer instigate nontumorigenic epithelial cells to form tumors in a Dicer-dependent manner. These findings offer opportunities for the development of exosomes based biomarkers and therapies.Entities:
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Year: 2014 PMID： 25446899 PMCID： PMC4254633 DOI： 10.1016/j.ccell.2014.09.005
Source DB: PubMed Journal: Cancer Cell ISSN： 1535-6108 Impact factor: 31.743