| Literature DB >> 25444656 |
Thirunavukkarasu Ashokkumar1, Durai Prabhu2, Ravi Geetha1, Kasivelu Govindaraju3, Ramar Manikandan2, Chinnasamy Arulvasu2, Ganesan Singaravelu4.
Abstract
An ethnopharmacological approach for biosynthesis of gold nanoparticles is being demonstrated using seed coat of Cajanus cajan. Medicinal value of capping molecule investigated for anticancer activity and results disclose its greater potential. The active principle of the seed coat [3-butoxy-2-hydroxypropyl 2-(2,4-dihydroxyphenyl) acetate] is elucidated. Rapid one-step synthesis yields highly stable, monodisperse (spherical) gold nanoparticles in the size ranging from 9 to 41 nm. Anticancer activity has been studied using liver cancer cells and cytotoxic mechanism has been evaluated using MTT, Annexin-V/PI Double-Staining Assay, Cell cycle, Comet assay and Flow cytometric analysis for apoptosis. The present investigation will open up a new possibility of functionalizing gold nanoparticles for apoptosis studies in liver cancer cells.Entities:
Keywords: Apoptosis; Cajanus cajan; Gold nanoparticles; Liver cancer; in vitro
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Year: 2014 PMID： 25444656 DOI： 10.1016/j.colsurfb.2014.09.051
Source DB: PubMed Journal: Colloids Surf B Biointerfaces ISSN： 0927-7765 Impact factor: 5.268