Literature DB >> 796660

Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae.

J H Cramer, F W Farrelly, R H Rownd.   

Abstract

The size and degree of homogeneity of the repetitive units in purified ribosomal DNA (gamma DNA) from Saccharomyces cerevisiae have been analyzed by restriction endonuclease digestion and heteroduplex mapping. Digestion of the gamma DNA with EcoRI yields seven fragments, digestion with Hind II+III yields five fragments, digestion with Hind III alone yields two fragments, and digestion with Sma I yields one fragment. The sum of the fragment molecular weights after digestion with each of the endonucleases is 5.5-5.6 x 10(6). When the DNA strands of the Sma I fragment are dissociated and reannealed, only homoduplexes are formed. We have concluded from these results that the repeating units in yeast ribosomal DNA are 5.6 x 10(6) datons and are homogeneous in size and composition.

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Year:  1976        PMID： 796660     DOI： 10.1007/bf00332897

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  23 in total

1.  Characterization of the DNA from bacteriophage P2-186 hybrids and physical mapping of the 186 chromosome.

Authors:  H B Younghusband; J B Egan; R B Inman
Journal:  Mol Gen Genet       Date:  1975-09-29

2.  On the distribution of 5s RNA cistrons on the genome of Saccharomyces cerevisiae.

Authors:  K Aarstad; T B Oyen
Journal:  FEBS Lett       Date:  1975-03-01       Impact factor: 4.124

3.  Isolation of high molecular weight DNA from yeast nuclei.

Authors:  M M Bhargava; J H Cramer; H O Halvorson
Journal:  Anal Biochem       Date:  1972-09       Impact factor: 3.365

4.  Location of ribosomal RNA cistrons in yeast.

Authors:  D B Finkelstein; J Blamire; J Marmur
Journal:  Nat New Biol       Date:  1972-12-27

5.  Letter: Location and arrangement of genes coding for ribosomal RNA in Saccharomyces cerevisiae.

Authors:  D B Kaback; M M Bhargava; H O Halvorson
Journal:  J Mol Biol       Date:  1973-10-05       Impact factor: 5.469

6.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

7.  Study of a haploid yeast strain with an unusually high rDNA content. II. Fractionation of the DNA in preparative Ag+-Cs2SO4 density gradients.

Authors:  O Karijord; T B Oyen
Journal:  Biochim Biophys Acta       Date:  1975-03-21

8.  Separation and characterization of mitochondrial DNA from yeast.

Authors:  K K Tewari; J Jayaraman; H R Mahler
Journal:  Biochem Biophys Res Commun       Date:  1965-10-26       Impact factor: 3.575

9.  Physiological variations in satellite components of yeast DNA detected by density gradient centrifugation.

Authors:  E Moustacchi; D H Williamson
Journal:  Biochem Biophys Res Commun       Date:  1966-04-06       Impact factor: 3.575

10.  Mitochondrial DNA in yeast and some mammalian species.

Authors:  G Corneo; C Moore; D R Sanadi; L I Grossman; J Marmur
Journal:  Science       Date:  1966-02-11       Impact factor: 47.728
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  30 in total

1.  Yeast L double-stranded ribonucleic acid is synthesized during the G1 phase but not the S phase of the cell cycle.

Authors:  V A Zakian; D W Wagner; W L Fangman
Journal:  Mol Cell Biol       Date:  1981-08       Impact factor: 4.272

2.  Arrangement of the genes coding for ribosomal ribonucleic acids in Neurospora crassa.

Authors:  S J Free; P W Rice; R L Metzenberg
Journal:  J Bacteriol       Date:  1979-03       Impact factor: 3.490

3.  Strain specific differences in ribosomal DNA from the fungus Schizophyllum commune.

Authors:  C A Specht; C P Novotny; R C Ullrich
Journal:  Curr Genet       Date:  1984-04       Impact factor: 3.886

4.  Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.

Authors:  R Anand; A Villasante; C Tyler-Smith
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

5.  A gene with specific and global effects on recombination of sequences from tandemly repeated genes in Saccharomyces cerevisiae.

Authors:  R L Keil; A D McWilliams
Journal:  Genetics       Date:  1993-11       Impact factor: 4.562

6.  Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA.

Authors:  J H Cramer; F W Farrelly; J T Barnitz; R H Rownd
Journal:  Mol Gen Genet       Date:  1977-03-16

7.  Meiotic mapping of yeast ribosomal deoxyribonucleic acid on chromosome XII.

Authors:  T D Petes
Journal:  J Bacteriol       Date:  1979-04       Impact factor: 3.490

8.  Yeast ribosomal DNA genes are located on chromosome XII.

Authors:  T D Petes
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

9.  Yeast RNA polymerase I binds preferentially to A+T-rich linkers in rDNA.

Authors:  O S Gabrielsen; T B Oyen
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

10.  Characterization of two types of yeast ribosomal DNA genes.

Authors:  T D Petes; L M Hereford; K G Skryabin
Journal:  J Bacteriol       Date:  1978-04       Impact factor: 3.490
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