Takahiro Tsukui1, Nozomi Nagano2, Myco Umemura1, Toshitaka Kumagai1, Goro Terai1, Masayuki Machida1, Kiyoshi Asai2. 1. Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo 135-0064, Japan, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido 062-8517, Japan, Fermlab Inc., Tokyo 135-0021, Japan, INTEC Inc., Tokyo 136-8637, Japan, and Department of Computational Biology, Graduate School of Frontier Sciences, the University of Tokyo, Chiba 277-8561, Japan. 2. Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo 135-0064, Japan, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido 062-8517, Japan, Fermlab Inc., Tokyo 135-0021, Japan, INTEC Inc., Tokyo 136-8637, Japan, and Department of Computational Biology, Graduate School of Frontier Sciences, the University of Tokyo, Chiba 277-8561, Japan Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo 135-0064, Japan, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido 062-8517, Japan, Fermlab Inc., Tokyo 135-0021, Japan, INTEC Inc., Tokyo 136-8637, Japan, and Department of Computational Biology, Graduate School of Frontier Sciences, the University of Tokyo, Chiba 277-8561, Japan.
Abstract
MOTIVATION: Ustiloxins A and B are toxic cyclic tetrapeptides, Tyr-Val/Ala-Ile-Gly (Y-V/A-I-G), that were originally identified from Ustilaginoidea virens, a pathogenic fungus affecting rice plants. Contrary to our report that ustiloxin B is ribosomally synthesized in Aspergillus flavus, a recent report suggested that ustiloxins are synthesized by a non-ribosomal peptide synthetase in U.virens. Thus, we analyzed the U.virens genome, to identify the responsible gene cluster. RESULTS: The biosynthetic gene cluster was identified from the genome of U.virens based on homologies to the ribosomal peptide biosynthetic gene cluster for ustiloxin B identified from A.flavus. It contains a gene encoding precursor protein having five Tyr-Val-Ile-Gly and three Tyr-Ala-Ile-Gly motifs for ustiloxins A and B, respectively, strongly indicating that ustiloxins A and B from U.virens are ribosomally synthesized. AVAILABILITY AND IMPLEMENTATION: Accession codes of the U.virens and A.flavus gene clusters in NCBI are BR001221 and BR001206, respectively. Supplementary data are available at Bioinformatics online.
MOTIVATION: Ustiloxins A and B are toxic cyclic tetrapeptides, Tyr-Val/Ala-Ile-Gly (Y-V/A-I-G), that were originally identified from Ustilaginoidea virens, a pathogenic fungus affecting rice plants. Contrary to our report that ustiloxin B is ribosomally synthesized in Aspergillus flavus, a recent report suggested that ustiloxins are synthesized by a non-ribosomal peptide synthetase in U.virens. Thus, we analyzed the U.virens genome, to identify the responsible gene cluster. RESULTS: The biosynthetic gene cluster was identified from the genome of U.virens based on homologies to the ribosomal peptide biosynthetic gene cluster for ustiloxin B identified from A.flavus. It contains a gene encoding precursor protein having five Tyr-Val-Ile-Gly and three Tyr-Ala-Ile-Gly motifs for ustiloxins A and B, respectively, strongly indicating that ustiloxins A and B from U.virens are ribosomally synthesized. AVAILABILITY AND IMPLEMENTATION: Accession codes of the U.virens and A.flavus gene clusters in NCBI are BR001221 and BR001206, respectively. Supplementary data are available at Bioinformatics online.
Authors: Devanna Pramesh; Muthukapalli K Prasannakumar; Kondarajanahally M Muniraju; H B Mahesh; H D Pushpa; Channappa Manjunatha; Alase Saddamhusen; E Chidanandappa; Manoj K Yadav; Masalavada K Kumara; Huded Sharanabasav; B S Rohith; Gaurab Banerjee; Anupam J Das Journal: 3 Biotech Date: 2020-07-19 Impact factor: 2.406
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Authors: Manuel Montalbán-López; Thomas A Scott; Sangeetha Ramesh; Imran R Rahman; Auke J van Heel; Jakob H Viel; Vahe Bandarian; Elke Dittmann; Olga Genilloud; Yuki Goto; María José Grande Burgos; Colin Hill; Seokhee Kim; Jesko Koehnke; John A Latham; A James Link; Beatriz Martínez; Satish K Nair; Yvain Nicolet; Sylvie Rebuffat; Hans-Georg Sahl; Dipti Sareen; Eric W Schmidt; Lutz Schmitt; Konstantin Severinov; Roderich D Süssmuth; Andrew W Truman; Huan Wang; Jing-Ke Weng; Gilles P van Wezel; Qi Zhang; Jin Zhong; Jörn Piel; Douglas A Mitchell; Oscar P Kuipers; Wilfred A van der Donk Journal: Nat Prod Rep Date: 2020-09-16 Impact factor: 15.111