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Literature DB >> 25414

Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography.

Abstract

High pressure reversed phase chromatography (RPC-5) at pH 12 was used for preparative separation of the complementary strands of the smaller DNA fragments which are generated by the Hae III restriction endonuclease digestion of Col El DNA. A single high pressure RPC-5 chromatographic step at neutral pH served to purify duplex fragments 70, 172, 250 and 440 base pairs long; each of these yielded two elution peaks upon chromatography under alkaline denaturing conditions.

Entities: Gene

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Year: 1978 PMID： 25414 PMCID： PMC341958 DOI： 10.1093/nar/5.1.13

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

2. Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.

Authors: S C Hardies; R D Wells
Journal: Proc Natl Acad Sci U S A Date: 1976-09 Impact factor: 11.205

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

Review 4. The role of DNA structure in genetic regulation.

Authors: R D Wells; R W Blakesley; S C Hardies; G T Horn; J E Larson; E Selsing; J F Burd; H W Chan; J B Dodgson; K F Jensen; I F Nes; R M Wartell
Journal: CRC Crit Rev Biochem Date: 1977

5. Separation of the complementary strands of DNA fragments on polyacrylamide gels.

Authors: A A Szalay; K Grohmann; R L Sinsheimer
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

6. Origin of replication of colicin E1 plasmid DNA.

Authors: J I Tomizawa; H Ohmori; R E Bird
Journal: Proc Natl Acad Sci U S A Date: 1977-05 Impact factor: 11.205

7. The isolation and sequence analysis of transfer RNA: the use of plaskon chromatography (RPC-5).

Authors: B Roe; K Marcu; B Dudock
Journal: Biochim Biophys Acta Date: 1973-08-10

8. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

9. The nucleotide sequence surrounding the origin of DNA replication of Col E1.

Authors: D Bastia
Journal: Nucleic Acids Res Date: 1977-09 Impact factor: 16.971

10. Anion-exchange chromatography on reversed-phase columns: isolation and assay of nucleosides, nucleotides, and oligonucleotides from nucleic acids and from cytoplasm.

Authors: R P Singhal
Journal: Biochim Biophys Acta Date: 1973-08-10

8 in total

Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography.

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

2. Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.

3. A new method for sequencing DNA.

Review 4. The role of DNA structure in genetic regulation.

5. Separation of the complementary strands of DNA fragments on polyacrylamide gels.

6. Origin of replication of colicin E1 plasmid DNA.

7. The isolation and sequence analysis of transfer RNA: the use of plaskon chromatography (RPC-5).

8. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

9. The nucleotide sequence surrounding the origin of DNA replication of Col E1.

10. Anion-exchange chromatography on reversed-phase columns: isolation and assay of nucleosides, nucleotides, and oligonucleotides from nucleic acids and from cytoplasm.

1. Specific chemical labeling of DNA fragments.

2. Electrophoretic strand separation of long DNAs with poly (U,G) in agarose gels.

3. Characterization of the 5'-terminal structure of simian virus 40 early mRNA's.

4. High-resolution liquid chromatography of DNA fragments on non-porous poly(styrene-divinylbenzene) particles.

5. Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.

6. Nucleotide sequence of the 18S-26S rRNA intergene region of the sea urchin.

7. Effect of DNA length on the nucleosome low salt transition.

8. Reverse-phase HPLC of DNA restriction fragments and ribooligonucleotides on uncoated Kel-F powder.