| Literature DB >> 25388395 |
Isobel Massie1, Marc Dziasko, Alvena Kureshi, Hannah J Levis, Louise Morgan, Michael Neale, Radhika Sheth, Victoria E Tovell, Amanda J Vernon, James L Funderburgh, Julie T Daniels.
Abstract
The limbal epithelial stem cell niche provides a unique, physically protective environment in which limbal epithelial stem cells reside in close proximity with accessory cell types and their secreted factors. The use of advanced imaging techniques is described to visualize the niche in three dimensions in native human corneal tissue. In addition, a protocol is provided for the isolation and culture of three different cell types, including human limbal epithelial stem cells from the limbal niche of human donor tissue. Finally, the process of incorporating these cells within plastic compressed collagen constructs to form a tissue-engineered corneal limbus is described and how immunohistochemical techniques may be applied to characterize cell phenotype therein.Entities:
Mesh：
Year: 2015 PMID： 25388395 PMCID： PMC4398336 DOI： 10.1007/978-1-4939-1785-3_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745